CAJ | 학술논문

目的探讨中国湖南汉族人群FGA-128C/G基因多态性与脑梗死的关系及其对血浆纤维蛋白原(fibrinogen,Fg)的影响.方法应用聚合酶链反应-限制性酶切片段长度多态性方法对中国湖南汉族194例脑梗死患者和114名与之年龄、性别等相匹配的健康体检者进行FGA-128C/G基因多态分析,并经基因测序证实.结果在中国湖南汉族人群中,FGA-128C/G存在CC、CG基因型,未发现GG基因型;两种基因型和等位基因频率在脑梗死组和对照组之间比较差异无统计学意义(P>0.05);Fg水平在两种基因型之间比较差异亦无统计学意义(P>0.05);通过对血浆Fg影响因素的多元回归分析发现,Fg水平与FGA-128C/G基因型无关(P>0.05),而随年龄增加而升高,男性高于女性(回归系数分别为0.079,-0.534).结论中国湖南汉族人群存在FGA-128C/G基因多态性;FGA-128C/G基因多态性与脑梗死患者血浆Fg水平增高无显著相关性,可能不是湖南汉族人群脑梗死的遗传易感基因;年龄和性别是湖南汉族人血浆Fg水平的主要影响因素.
목적 탐토중국호남한족인군FGA-128C/G기인다태성여뇌경사적관계급기대혈장섬유단백원(fibrinogen,Fg)적영향.방법 응용취합매련반응-한제성매절편단장도다태성방법대중국호남한족194례뇌경사환자화114명여지년령、성별등상필배적건강체검자진행FGA-128C/G기인다태분석,병경기인측서증실.결과 재중국호남한족인군중,FGA-128C/G존재CC、CG기인형,미발현GG기인형;량충기인형화등위기인빈솔재뇌경사조화대조조지간비교차이무통계학의의(P>0.05);Fg수평재량충기인형지간비교차이역무통계학의의(P>0.05);통과대혈장Fg영향인소적다원회귀분석발현,Fg수평여FGA-128C/G기인형무관(P>0.05),이수년령증가이승고,남성고우녀성(회귀계수분별위0.079,-0.534).결론 중국호남한족인군존재FGA-128C/G기인다태성;FGA-128C/G기인다태성여뇌경사환자혈장Fg수평증고무현저상관성,가능불시호남한족인군뇌경사적유전역감기인;년령화성별시호남한족인혈장Fg수평적주요영향인소.
Objective To investigate the relationship of FGA gene 128C/G polymorphism and cerebral infarction (CD and evaluate the effect of FGA- 128C/G polymorphism on plasma fibrinogen in Hunan Hans. Methods FGA -128C/G polymorphism was detected by (polymerase chian reaction-restrction fragment length polymorphism, PCR-RFLP) and DNA sequencing in 194 CI patients and 114 healthy controls. Results There were CG and CC genotypes in the FGA- 128C/G locus. No GG genotype was observed in Hunan Hans. There was no significant difference in genotype and allele frequencies between the controls and CI group (P>0. 05), and statistically significant difference was not found in fibrinogen (Fg) level between the CG and CC genotypes (P>0. 05). After analyzing blood plasma Fg using the influencing factor multiple regression analysis, it was shown that the Fg level had no relationship with the FGA- 128C/ G genotype, but it increased with age. And the Fg level in males was higher than that in females. Conclusion There was FGA gene 128C/G polymorphism in the Hunan Han population. There was no association of this polymorphism with the increased Fg level of CI patient in the population. FGA-128C/G might not be the predisposing gene of CI in Hunan Han population. The age and sex were the major factors affecting the plasma Fg level in this population.