中国农业科技导报
中國農業科技導報
중국농업과기도보
REVIEW OF CHINA AGRICULTURAL SCIENCE AND TECHNOLOGY
2009年
3期
81-87
,共7页
郑怀忠%陈发河%李淑燕%孙君社%刘萍
鄭懷忠%陳髮河%李淑燕%孫君社%劉萍
정부충%진발하%리숙연%손군사%류평
亚硝酸还原酶%紫外诱变%发酵%条件优化
亞硝痠還原酶%紫外誘變%髮酵%條件優化
아초산환원매%자외유변%발효%조건우화
nitrite reductase%UV%fermentation%condition optimization
通过紫外线对巨大芽孢杆菌MPF-906进行了诱变处理,提高了产酶能力,变异株Z85酶活达到28.76 U/mL.进一步对其发酵条件进行研究,优化了培养基和培养条件.优化的培养基为:葡萄糖1.5%,牛肉膏0.2%,硫酸铵0.1%,K2HPO4 0.135%,KH2PO4 0.07%,NaCl 0.1%;无机盐溶液10 mL/L(即MgSO4 · 7H2O 0.04%、MnSO4 · H2O 0.001%、CaCl2 · 2H2O 0.002%).发酵条件为:摇床转速160 rpm,接种量4%,初始pH 6.5,30 ℃发酵20 h.在此最优化条件下,亚硝酸还原酶的酶活达到45.94 U/mL.
通過紫外線對巨大芽孢桿菌MPF-906進行瞭誘變處理,提高瞭產酶能力,變異株Z85酶活達到28.76 U/mL.進一步對其髮酵條件進行研究,優化瞭培養基和培養條件.優化的培養基為:葡萄糖1.5%,牛肉膏0.2%,硫痠銨0.1%,K2HPO4 0.135%,KH2PO4 0.07%,NaCl 0.1%;無機鹽溶液10 mL/L(即MgSO4 · 7H2O 0.04%、MnSO4 · H2O 0.001%、CaCl2 · 2H2O 0.002%).髮酵條件為:搖床轉速160 rpm,接種量4%,初始pH 6.5,30 ℃髮酵20 h.在此最優化條件下,亞硝痠還原酶的酶活達到45.94 U/mL.
통과자외선대거대아포간균MPF-906진행료유변처리,제고료산매능력,변이주Z85매활체도28.76 U/mL.진일보대기발효조건진행연구,우화료배양기화배양조건.우화적배양기위:포도당1.5%,우육고0.2%,류산안0.1%,K2HPO4 0.135%,KH2PO4 0.07%,NaCl 0.1%;무궤염용액10 mL/L(즉MgSO4 · 7H2O 0.04%、MnSO4 · H2O 0.001%、CaCl2 · 2H2O 0.002%).발효조건위:요상전속160 rpm,접충량4%,초시pH 6.5,30 ℃발효20 h.재차최우화조건하,아초산환원매적매활체도45.94 U/mL.
The nitrite reductase production strain B.megaterium MPF-906 was treated with UV. The high nitrite reductase producing mutant Z85 was obtained and its nitrite reductase activity was 28.76 U/mL. The effects of compositions of culture medium and cultural conditions on nitrite reductase production were investigated. The optimal culture media are: glucose 1.5%, beef extract 0.2%, (NH4)2SO4 0.1%, K2HPO4 0.135%, KH2PO4 0.07%, NaCl 0.1%, MgSO4 · 7H2O 0.04%, MnSO4 · H2O 0.001%, CaCl2 · 2H2O 0.002%. The fermentation conditions are 30℃ for 20 h, 160 rpm, initial pH 6.5 and 4% of inoculation. Under this optimized conditions, the nitrite reductase activity reached 45.94 U/mL.
