CAJ | 학술논문

应用均匀设计法筛选钻天柳最适合的培养基.结果表明,钻天柳组织培养的不同阶段需要不同的培养基.最适合的嫩叶柄愈伤组织诱导培养基为:MS+KT 2.00mg/L+NAA 0.05mg/L,诱导率为95.5%;嫩叶柄愈伤组织不定芽的分化培养基为:MS+6-BA 3.90mg/L+NAA 0.01mg/L,分化率为98%;生根培养基为:1/2MS+IBA 0.07mg/L,生根率达99%以上.成功建立了钻天柳嫩叶柄再生体系.
응용균균설계법사선찬천류최괄합적배양기.결과표명,찬천류조직배양적불동계단수요불동적배양기.최괄합적눈협병유상조직유도배양기위:MS+KT 2.00mg/L+NAA 0.05mg/L,유도솔위95.5%;눈협병유상조직불정아적분화배양기위:MS+6-BA 3.90mg/L+NAA 0.01mg/L,분화솔위98%;생근배양기위:1/2MS+IBA 0.07mg/L,생근솔체99%이상.성공건립료찬천류눈협병재생체계.
An experiment was conducted to study the optimal culture medium for Chosenia arbutifolia by uniform design using tender leafstalks as explants. Results showed that C. arbutifolia required different kinds of culture media in different culti-vation phases. The most suitable culture mediums were obtained as MS supplemented with KT 2.00 mg/L and NAA 0.05 mg/L for callus induction with an induction rate of 95.5% , MS with 6-BA 3.90 mg/L and NAA 0.01 mg/L for callus mul-tiplication with a multiplication rate of 98% , and 1/2MS with IBA 0.07 mg/L for rooting with a rooting rate of more than 99%. Plant regeneration system in tender leafstalks of C. arbutifolia was successfully established.