CAJ | 학술논문

用超临界CO_2流体色谱分离丁香酚与异丁香酚两个双键位置异构体.以分离度为指标,考察改性剂、柱压、柱温3个因素对分离的影响,优化使用硅胶色谱柱和氰基色谱梓分离丁香酚与异丁香酚的色谱条件.结果表明,使用氰基柱的分离效果更好,异丁香酚的顺、反异构体也得到分离.在紫外检测波长283nm、流动相流速1.5ml/min的条件下,进一步考察进样量对分离的影响,最终确定分离丁香酚、异丁香酚的色谱条件为氰基柱(250mm×4.6mm,5μm),改性剂为体积分数8%的甲醇、柱温40℃、柱压8MPa、进样质量体积10μl、样品质量浓度43.7mg/ml.此时分离度241,分离时间大约为7min.
용초림계CO_2류체색보분리정향분여이정향분량개쌍건위치이구체.이분리도위지표,고찰개성제、주압、주온3개인소대분리적영향,우화사용규효색보주화청기색보재분리정향분여이정향분적색보조건.결과표명,사용청기주적분리효과경호,이정향분적순、반이구체야득도분리.재자외검측파장283nm、류동상류속1.5ml/min적조건하,진일보고찰진양량대분리적영향,최종학정분리정향분、이정향분적색보조건위청기주(250mm×4.6mm,5μm),개성제위체적분수8%적갑순、주온40℃、주압8MPa、진양질량체적10μl、양품질량농도43.7mg/ml.차시분리도241,분리시간대약위7min.
Supercritical CO_2 fluid chromatography (SFC) was used to separate isomers, eugenol and isoeugenol in this paper. Effects of modifier, column pressure and column temperature on separation efficiency was evaluated by resolution factor under optimal silica column or cyano column. Results indicated cyano column provided better separation efficiency due to successful separation of trans- and cis-isomers of isoeugenol. The optimal separation condition for eugenol and isoeugenol was cyano column (250 mm×4.6 mm, 5μm), UV detection wavelength at 283 nm, 8% modifier in methanol as mobile phase, 1.5 ml/min of flow rate for mobile phase, 40 ℃ of column temperature, 8 MPa column pressure, 10 μl of sample loading amount, and 43.7 mg/ml sample. The resolution factor was 2.41 with 7 min of running time under this optimal separation condition.