CAJ | 학술논문

目的探讨青藤碱( sinomenine ,Sin)对糖尿病大鼠脑缺血再灌注损伤单核细胞趋化蛋白-1(MCP-1)和肿瘤坏死因子-α(TNF-α)含量的影响.方法采用高脂高糖饮食加腹腔注射小剂量链脲霉素建立糖尿病大鼠模型,造模成功后将大鼠随机分为假手术组、缺血再灌注组、Sin低剂量治疗组和Sin高剂量治疗组.线栓法建立局灶性脑缺血再灌注模型.Sin低(30 mg/kg)、高剂量(60 mg/kg)治疗组于术前30 min分别给予大鼠腹腔注射.采用酶联免疫吸附实验( ELISA)法检测缺血90 min再灌注24 h大鼠额顶部皮质MCP-1和TNF-α含量的变化,并进行2,3,5-三苯基氯化四氮唑(TTC)染色和HE染色观察脑梗塞体积及病理形态学变化.结果 ①脑缺血再灌注24h,缺血再灌注组MCP-1和TNF-α含量明显升高,与假手术组比较,差异具有显著性(均P<0.05);与缺血再灌注组比较,Sin高、低剂量治疗组均显著减少MCP-1和TNF-α含量;高、低剂量组之间差异亦具有显著性(P<0.05);②Sin治疗组脑梗塞体积较缺血再灌注组减小,高、低剂量组之间差异亦具有显著性(P<0.05);③Sin高、低剂量治疗组脑组织缺血损伤病理学改变明显轻于缺血再灌注组,Sin高剂量治疗组缺血改变亦轻于低剂量治疗组.结论 Sin对糖尿病大鼠缺血再灌注脑损伤具有保护作用,其机制可能与降低缺血再灌注损伤脑组织MCP-1和TNF-α含量,抑制再灌注损伤炎症反应有关.
목적 탐토청등감( sinomenine ,Sin)대당뇨병대서뇌결혈재관주손상단핵세포추화단백-1(MCP-1)화종류배사인자-α(TNF-α)함량적영향.방법 채용고지고당음식가복강주사소제량련뇨매소건립당뇨병대서모형,조모성공후장대서수궤분위가수술조、결혈재관주조、Sin저제량치료조화Sin고제량치료조.선전법건립국조성뇌결혈재관주모형.Sin저(30 mg/kg)、고제량(60 mg/kg)치료조우술전30 min분별급여대서복강주사.채용매련면역흡부실험( ELISA)법검측결혈90 min재관주24 h대서액정부피질MCP-1화TNF-α함량적변화,병진행2,3,5-삼분기록화사담서(TTC)염색화HE염색관찰뇌경새체적급병리형태학변화.결과 ①뇌결혈재관주24h,결혈재관주조MCP-1화TNF-α함량명현승고,여가수술조비교,차이구유현저성(균P<0.05);여결혈재관주조비교,Sin고、저제량치료조균현저감소MCP-1화TNF-α함량;고、저제량조지간차이역구유현저성(P<0.05);②Sin치료조뇌경새체적교결혈재관주조감소,고、저제량조지간차이역구유현저성(P<0.05);③Sin고、저제량치료조뇌조직결혈손상병이학개변명현경우결혈재관주조,Sin고제량치료조결혈개변역경우저제량치료조.결론 Sin대당뇨병대서결혈재관주뇌손상구유보호작용,기궤제가능여강저결혈재관주손상뇌조직MCP-1화TNF-α함량,억제재관주손상염증반응유관.
Objective To study the effect of sinomenine(Sin) on the contents of MCP-1 and TNF-αafter cerebral ischemia reperfusion(I/R) injury in diabetic rats.Methods In this experiment, rat model of diabetes mellitus was made by high sucrose , fat diet and streptozotion injection.Diabetic rats were randomly divided into 4 groups, namely sham operated group, I/R group,low dose Sin treated group and high dose Sin treated group. The focal middle cerebral artery occlusion(MCAO) model was made by suture-occluded method. Sin were given intraperitoneally to rats 30min before focal cerebral ischemia operation. After 90min MCAO following 24h of reperfusion,we investigated the contents of MCP-1 and TNF-α in ischemic frontal and parietal cortex . HE staining and TTC staining were also investigated. Results ①Compared with sham operated group , the contents of MCP-1 and TNF-α were increased at 24h of reperfusion in the ischemic territory(P<0.05). Compared with I/R group, Sin dose-dependently reduced the contents of MCP-1 and TNF-α (all P<0.05).②Compared with I/R group,cerebral infarction volume in low and highdose Sin treated groups was decreased dose-dependently (all P<0.05). ③The change of ischemic impairment in low or high dose Sin treated group was lighter than that of I/R group,and high dose Sin treated group was lighter than that of low dose Sin treated group.Conclusion Sin may reduced cerebral ischemia-reperfusion injury by decreasing the contents of MCP-1 and TNF-α in diabetic rats.