CAJ | 학술논문

斜带石斑鱼(Epinephelus coioides)为我国南方近海霞要网箱养殖海水鱼品种之一,而石斑鱼苗种繁育成败对其养殖规模化有着重要影响.为此,本研究比较了3种不同水培育方式下斜带石斑龟孵化池水质变化特征.孵化在水泥池中进行,池规格为2.5 m×4.0 m×1.1 m.试验采用益生菌和虾片作为培水材料,益生菌总活菌浓度为1.O×108 cells/mL.在孵化前1周,每天同时等量往孵化池中加入益生菌和虾片2次,至试验结束,添加时间分别为早上7:00和下午13:00.处理组1、2、3益生菌日添加量分别为每池160、320、320 mL,虾片日添加量分别为每池8、8、16 g.每组设2个重复.石斑鱼受精卵孵化后持续培育27 d,并分别在孵化后第3、8、13、18、23、27 d检测水池中pH值和溶氧、总磷、活性磷、氨氮、硝酸氮、弧硝酸氮含昔.结果表明:在孵化后第3d,水体中pH值从8.06下降至7.03,之后义上升至初始值.试验期间,溶解氧水平维持在6.86～7.99 mg/L之间.总磷和活性磷水平随培养时间延长而增加.在孵化后第13 d,亚硝酸氮水平开始升高,并在孵化后第23 d达到峰值(约0.57 mg/L).硝酸氮含量也从孵化后第13 d开始上升.各处殚组氨氮含量均在孵化后第18 d上升至最高值.试验期间,处理组2亚硝酸氮及硝酸氮水平均低于处理组1及处理组2.因此,基于水体亚硝酸氮及硝酸氮为评价指标,斜带石斑鱼孵化池中日添加益生菌及虾片适宜浓度分别为40 mL/m3和1g/m3.另外,为避免可能发生的石斑鱼鱼苗亚硝酸盐中毒,应在孵化后第23 d往池中注入适当新水.
사대석반어(Epinephelus coioides)위아국남방근해하요망상양식해수어품충지일,이석반어묘충번육성패대기양식규모화유착중요영향.위차,본연구비교료3충불동수배육방식하사대석반구부화지수질변화특정.부화재수니지중진행,지규격위2.5 m×4.0 m×1.1 m.시험채용익생균화하편작위배수재료,익생균총활균농도위1.O×108 cells/mL.재부화전1주,매천동시등량왕부화지중가입익생균화하편2차,지시험결속,첨가시간분별위조상7:00화하오13:00.처리조1、2、3익생균일첨가량분별위매지160、320、320 mL,하편일첨가량분별위매지8、8、16 g.매조설2개중복.석반어수정란부화후지속배육27 d,병분별재부화후제3、8、13、18、23、27 d검측수지중pH치화용양、총린、활성린、안담、초산담、호초산담함석.결과표명:재부화후제3d,수체중pH치종8.06하강지7.03,지후의상승지초시치.시험기간,용해양수평유지재6.86～7.99 mg/L지간.총린화활성린수평수배양시간연장이증가.재부화후제13 d,아초산담수평개시승고,병재부화후제23 d체도봉치(약0.57 mg/L).초산담함량야종부화후제13 d개시상승.각처탄조안담함량균재부화후제18 d상승지최고치.시험기간,처리조2아초산담급초산담수평균저우처리조1급처리조2.인차,기우수체아초산담급초산담위평개지표,사대석반어부화지중일첨가익생균급하편괄의농도분별위40 mL/m3화1g/m3.령외,위피면가능발생적석반어어묘아초산염중독,응재부화후제23 d왕지중주입괄당신수.
This study compared and discussed response of water quality in concrete nursery ponds (W 2.5 m×L 4.0 m×H 1.1 m) applied for grouper Epinephelus coioides hatching under three different water breeding regimes (group 1, 2, 3). Pepared effective microorganisms (a total active bacterium concentration of 1.0×103 cells/mL solution (EMS) together with shrimp chip (SC) were daily added. Daily addition levels of EMS for group 1, group 2 and group 3 were respectively 160 mL, 320 mL and 320 mL per pond. For SC,aily addition levels were respectively 8 g, 8 g and 16 g per pond. The experiment continued 27 days. Water quality was measured during the whole experimental period, pH values decreased from 8.06 to 7.45 on 3 days after hatching (DAH), and then increased to the initial level. Dissolved oxygen (DO) levels varied at 6.86～7.99 mg/L. Total phosphate (TP) had an increasing tendency with larvae cluture time,and similar results were observed in for Soluble reactive phosphate (SRP). On 13th NO2-N values rapidly rose and reached a peak (about 0.57 rag/L) on 23th. NO3-N values started to increase on 13th. NH3-N values in all groups had a peak on 18th. Overall, daily additions of EMS and SC at concentrations of 40 mL/m3 and 1 g/m3 would be optimal for water breeding of grouper nursery ponds, and new seawater should be exchanged into nursery ponds on 13th.