作物学报
作物學報
작물학보
ACTA AGRONOMICA SINICA
2010年
12期
2073-2083
,共11页
高清松%杨泽峰%周勇%张丹%闫成海%梁国华%徐辰武
高清鬆%楊澤峰%週勇%張丹%閆成海%樑國華%徐辰武
고청송%양택봉%주용%장단%염성해%량국화%서신무
玉米%类ABC1基因%克隆%镉应答%非生物胁迫
玉米%類ABC1基因%剋隆%鎘應答%非生物脅迫
옥미%류ABC1기인%극륭%력응답%비생물협박
Maize%ABC1-like gene%Cloning%Cadmium response%Abiotic stress
镉是一种非必需的重金属元素,对动植物有严重毒害作用.几个与ABC1(activity of the bcl complex)家族有关的基因参与植物镉胁迫的应答.本研究从玉米中克隆并鉴定了一个类ABC1基因,命名为ZmABC1-10.该基因cDNA全长2519 bp,包含一个2250 bp的开放阅读框,编码一个预测的叶绿体膜蛋白.启动子顺式元件扫描发现该基因含有大量的非生物胁迫、光以及植物激素应答元件.表达模式分析表明,该基因主要在叶片、茎秆等绿色组织中表达.镉处理实验表明,该基因能够被诱导并且受植物发育时期的调控.除镉之外,该基因还受多种非生物因素包括ABA、H2O2、干旱和黑暗的共同调控.此外,本研究利用基因组序列信息共鉴定出19个玉米ABC1基因.对植物界8个代表性物种中148个ABC1蛋白进行系统发育分析表明,在长期进化过程中植物ABC1蛋白已经发生了分化;物种特异性扩张是植物中该家族进化的主要动力.这些结果表明ZmAbcl-10是一个镉应答因子并且可能在植物对非生物胁迫的适应中发挥重要作用.
鎘是一種非必需的重金屬元素,對動植物有嚴重毒害作用.幾箇與ABC1(activity of the bcl complex)傢族有關的基因參與植物鎘脅迫的應答.本研究從玉米中剋隆併鑒定瞭一箇類ABC1基因,命名為ZmABC1-10.該基因cDNA全長2519 bp,包含一箇2250 bp的開放閱讀框,編碼一箇預測的葉綠體膜蛋白.啟動子順式元件掃描髮現該基因含有大量的非生物脅迫、光以及植物激素應答元件.錶達模式分析錶明,該基因主要在葉片、莖稈等綠色組織中錶達.鎘處理實驗錶明,該基因能夠被誘導併且受植物髮育時期的調控.除鎘之外,該基因還受多種非生物因素包括ABA、H2O2、榦旱和黑暗的共同調控.此外,本研究利用基因組序列信息共鑒定齣19箇玉米ABC1基因.對植物界8箇代錶性物種中148箇ABC1蛋白進行繫統髮育分析錶明,在長期進化過程中植物ABC1蛋白已經髮生瞭分化;物種特異性擴張是植物中該傢族進化的主要動力.這些結果錶明ZmAbcl-10是一箇鎘應答因子併且可能在植物對非生物脅迫的適應中髮揮重要作用.
력시일충비필수적중금속원소,대동식물유엄중독해작용.궤개여ABC1(activity of the bcl complex)가족유관적기인삼여식물력협박적응답.본연구종옥미중극륭병감정료일개류ABC1기인,명명위ZmABC1-10.해기인cDNA전장2519 bp,포함일개2250 bp적개방열독광,편마일개예측적협록체막단백.계동자순식원건소묘발현해기인함유대량적비생물협박、광이급식물격소응답원건.표체모식분석표명,해기인주요재협편、경간등록색조직중표체.력처리실험표명,해기인능구피유도병차수식물발육시기적조공.제력지외,해기인환수다충비생물인소포괄ABA、H2O2、간한화흑암적공동조공.차외,본연구이용기인조서렬신식공감정출19개옥미ABC1기인.대식물계8개대표성물충중148개ABC1단백진행계통발육분석표명,재장기진화과정중식물ABC1단백이경발생료분화;물충특이성확장시식물중해가족진화적주요동력.저사결과표명ZmAbcl-10시일개력응답인자병차가능재식물대비생물협박적괄응중발휘중요작용.
Cadmium is a non-essential heavy metal that is extremely toxic to plants and animals. Previous studies have shown that several proteins associated with the Activity of the bc1 complex (ABC1) protein family participate in plant responses to cadmium.Here we presented the cloning and characterization of an ABCl-like gene, ZmABC1-10, from maize (Zea mays L.). The full-length 2 519 bp cDNA of maize ABC1-10 gene contained an open reading frame (ORF) of 2 250 bp encoding a membrane-binding protein with a predicted localization in the chloroplast. A promoter scan detected numerous cis-elements implicated in abiotic stress,light, and phytohormone responses. Expression profile analysis indicated most expression of this gene occurred in green tissues.Cadmium treatment revealed that expression of this gene could be induced and was correlated with plant development. In addition to cadmium, ZmABC1-10 expression was also affected by a broad range of abiotic factors, such as ABA, H2O2, drought and darkness. A total of 19 members of maize ABC1 family were identified with the B73 maize genomic sequence. Phylogenetic analysis using 148 ABC1 proteins from 8 representative species of plant kingdom revealed that divergence occurred and species-specific expansion contributed to the evolution of this family in plants. Collectively, our data suggest that ZmAbcl-10 is a cadmiumesponsive factor and may play potential roles in the plant adaption to diverse abiotic stresses.