CAJ | 학술논문

目的了解慢性乙型肝炎(乙肝)及乙肝肝硬化患者血清细胞因子巨噬细胞移动抑制因子(MIF)表达水平,探讨血清MIF水平与Ⅲ型前胶原肽(PⅢP)、组织金属蛋白酶抑制剂-1(TIMP-1)的相关性.方法选择44例慢性乙肝患者(肝炎组)、44例乙肝肝硬化患者(肝硬化组)和30名健康者(对照组),取静脉血并应用ELISA方法检测其血清中细胞因子MIF浓度,分析实验组血清MIF水平与PⅢP、TIMP-1相关性.两组间均数比较采用t检验,MIF水平与ALT、AST、TBil、PTA、PⅢP及TIMP-1关系采用直线相关分析.结果慢性乙肝组及肝硬化组血清MIF、PⅢP及TIMP-1的浓度比健康对照组升高.差异均有统计学意义(t=12.87、5.28、10.98,t=11.22、14.84、11.17)均P<0.05),但该3种细胞因子在慢性乙肝及肝硬化患者之间差异无统计学意义(t=-1.05、1.52、-2.07;均P>0.05);MIF水平与ALT、AST、TBil及PTA均无相关性;HBeAg阳性和高病毒载量(>1×105拷贝/mL)慢性乙肝患者体内MIF水平高于HBeAg阴性和低病毒载量(≤1×105拷贝/mL)患者.MIF水平与PⅢP水平在慢性乙肝组和肝硬化组中均存在正相关(r=0.603,P<0.05;r=0.415,P<0.05);MIF水平与TIMP-1水平在慢性乙肝组中存在正相关(r=0.458,P<0.05),而在肝硬化组无相关性(r=0.210,P>0.05),PⅢ P与TIMP-1在慢性乙肝组及肝硬化组均存在相关性(r=0.649,P<0.05)r=0.424,P<0.05).结论慢性乙肝及肝硬化患者体内MIF水平明显升高,MIF的早期生成可能与病毒复制有关,与肝功能无关;MIF参与了肝炎,肝纤维化及肝硬化形成过程,可反应肝硬化程度.
목적 료해만성을형간염(을간)급을간간경화환자혈청세포인자거서세포이동억제인자(MIF)표체수평,탐토혈청MIF수평여Ⅲ형전효원태(PⅢP)、조직금속단백매억제제-1(TIMP-1)적상관성.방법 선택44례만성을간환자(간염조)、44례을간간경화환자(간경화조)화30명건강자(대조조),취정맥혈병응용ELISA방법검측기혈청중세포인자MIF농도,분석실험조혈청MIF수평여PⅢP、TIMP-1상관성.량조간균수비교채용t검험,MIF수평여ALT、AST、TBil、PTA、PⅢP급TIMP-1관계채용직선상관분석.결과 만성을간조급간경화조혈청MIF、PⅢP급TIMP-1적농도비건강대조조승고.차이균유통계학의의(t=12.87、5.28、10.98,t=11.22、14.84、11.17)균P<0.05),단해3충세포인자재만성을간급간경화환자지간차이무통계학의의(t=-1.05、1.52、-2.07;균P>0.05);MIF수평여ALT、AST、TBil급PTA균무상관성;HBeAg양성화고병독재량(>1×105고패/mL)만성을간환자체내MIF수평고우HBeAg음성화저병독재량(≤1×105고패/mL)환자.MIF수평여PⅢP수평재만성을간조화간경화조중균존재정상관(r=0.603,P<0.05;r=0.415,P<0.05);MIF수평여TIMP-1수평재만성을간조중존재정상관(r=0.458,P<0.05),이재간경화조무상관성(r=0.210,P>0.05),PⅢ P여TIMP-1재만성을간조급간경화조균존재상관성(r=0.649,P<0.05)r=0.424,P<0.05).결론 만성을간급간경화환자체내MIF수평명현승고,MIF적조기생성가능여병독복제유관,여간공능무관;MIF삼여료간염,간섬유화급간경화형성과정,가반응간경화정도.
Objective To investigate the level of serum macrophage migration inhibitory factor (MIF) and its correlation with serum precollagen Ⅲ peptide (PⅢP) and tissue inhibitor of metalloproteinase (TIMP)-1 in patients with chronic hepatitis B (CHB) and hepatitis B virus (HBV)-related cirrhosis. Methods Forty-four CHB patients (hepatitis B group), 44 patients with HBV-related cirrhosis (cirrhosis group) and 30 healthy controls (control group) were enrolled in this study. The venous blood was collected and MIF level was detected by enzyme-linked immunosorbent assay (ELISA). Correlations between MIF and PⅢP, TIMP-1 were analyzed in observed groups. Comparison between groups was done using t test. The correlations between MIF level and alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TBil), plasma thromboplastin antecedent (PTA), PⅢP and TIMP-1 were analyzed by rectilinear correlation. Results The levels of serum MIF, PⅢP and TIMP-1 in CHB group and cirrhosis group were all significantly higher than those in control group (t=12.87,5.28, 10.98,t=11.22,14.84,11.17;all P<0.05), while there were no significant differences between CHB group and cirrhosis group (t= -1.05,1.52,--2.07;all P>0.05). There was no correlation between MIF level and ALT, AST, TBil and PTA. MIF level in CHB patients with hepatitis B e antigen (HBeAg) positive and high viral load were both higher than that in patients with HBeAg negative and low viral load. MIF level was both positively correlated with PⅢP level in CHB group and cirrhosis group (r=0. 603, P<0.05 and r=0. 415, P<0. 05, respectively). MIF level was also positively correlated with TIMP-1 level in CHB group (r=0. 458, P<0.05), while not correlated in cirrhosis group (r=0. 210, P>0.05). Levels of PⅢP and T1MP-1 were both correlated in CHB group and cirrhosis group (r=0. 849, P< 0.05 and r=0. 424, P<0.05, respectively). Conclusions The levels of serum MIF are significantly increased both in patients with CHB and cirrhosis. The early production of MIF might be related with viral replication, but not with liver function. MIF participates in formations of hepatitis, liver fibrosis and cirrhosis, which could reflect the degree of liver cirrhosis.