CAJ | 학술논문

目的观察1,25-二羟基维生素D3[1,25(OH):D3]对白血病细胞株K562细胞周期及细胞凋亡的作用.方法 Western印迹检测维生素D受体(VDR)在K562细胞的表达;四噻唑蓝法(MTT)、AO/EB、流式细胞仪分析细胞生长抑制率、细胞凋亡率及细胞周期.结果 (1)K66a细胞核阳性表达VDR;(2)0-10-6mol/L浓度的1,25(OH)2D3呈浓度依赖性抑制K562细胞增殖,10-8mol/L 1,25(OH)2D3明显抑制K562细胞增殖,促进凋亡,细胞周期阻滞主要发生在G2期或M早期,凋亡率从4.1%(对照组)增至26.5%(P<0.01).结论 1,25(OH)2D3可明显抑制K562细胞增殖,促进细胞凋亡.
목적 관찰1,25-이간기유생소D3[1,25(OH):D3]대백혈병세포주K562세포주기급세포조망적작용.방법 Western인적검측유생소D수체(VDR)재K562세포적표체;사새서람법(MTT)、AO/EB、류식세포의분석세포생장억제솔、세포조망솔급세포주기.결과 (1)K66a세포핵양성표체VDR;(2)0-10-6mol/L농도적1,25(OH)2D3정농도의뢰성억제K562세포증식,10-8mol/L 1,25(OH)2D3명현억제K562세포증식,촉진조망,세포주기조체주요발생재G2기혹M조기,조망솔종4.1%(대조조)증지26.5%(P<0.01).결론 1,25(OH)2D3가명현억제K562세포증식,촉진세포조망.
Objective To investigate effects of 1,25-Dihydroxyvitamin D3 on the cell cycle and apoptosis of K652 cells. Methods Vitamin D receptor (VDR) expression was identified by western blot analysis. Cell growth, apoptosis and cell cycle were evaluated by MTT assay, acridine orange/ethidium bromide (AO/EB) and flow cytometry. Results VDR was present in K562 cell nucleus. 10-8mol/L 1, 25-Dihydroxyvitamin D3 could markedly inhibit K562 cells growth and induced cell apoptosis. Most of cells were arrested in G2/M phase, the ratio of apoptosis increased from 4.1% in control group to 26.5 % in treatment group (P<0.01). Conclusion 1,25-Dihydrexyvitamin D3 exhibited obvious inhibitory effects on cell proliferation and cell cycle, and induced apoptosis in K562 cells.