国际呼吸杂志
國際呼吸雜誌
국제호흡잡지
INTERNATIONAL JOURNAL OF RESPIRATION
2012年
4期
245-248
,共4页
蓝秀%王超%辛彩霞%宋本艳%王鸿程%吕祝庆%吕伟玲%孙德彬
藍秀%王超%辛綵霞%宋本豔%王鴻程%呂祝慶%呂偉玲%孫德彬
람수%왕초%신채하%송본염%왕홍정%려축경%려위령%손덕빈
肺腺癌%肿瘤标志物%信号转导和转录激活因子3%癌胚抗原
肺腺癌%腫瘤標誌物%信號轉導和轉錄激活因子3%癌胚抗原
폐선암%종류표지물%신호전도화전록격활인자3%암배항원
Lung adenocarcinoma%Tumor marker%Signal transducer and activator of trascription 3%Carcinoembryonic antigen
目的 探讨信号转导和转录激活因子3( STAT3)的临床应用价值.方法 利用电化学发光免疫法检测人肺腺癌细胞A549和人胚胎成纤维细胞MRC-5培养3d的培养液中癌胚抗原(CEA)的表达水平.采用荧光定量PCR法检测人肺腺癌细胞A549和人胚胎成纤维细胞MRC-5中STAT3mRNA、CEA mRNA的表达.结果 CEA在A549细胞培养液中的表达水平高于MRC-5细胞培养液[(31.38±30.58) ng/Lvs (3.10±3.70) ng/L,P＜0.05]；STAT3 mRNA、CEA mRNA在A549细胞中的表达水平(6.96±3.37,5.68±2.70)均明显高于MRC-5细胞(1.01±0.19,1.02±0.21)(P＜0.01),且在A549细胞中STAT3和CEA的表达均呈正相关(r=0.521,P=0.047).结论 STAT3有望成为一种新的诊断肺腺癌的肿瘤标志物.
目的 探討信號轉導和轉錄激活因子3( STAT3)的臨床應用價值.方法 利用電化學髮光免疫法檢測人肺腺癌細胞A549和人胚胎成纖維細胞MRC-5培養3d的培養液中癌胚抗原(CEA)的錶達水平.採用熒光定量PCR法檢測人肺腺癌細胞A549和人胚胎成纖維細胞MRC-5中STAT3mRNA、CEA mRNA的錶達.結果 CEA在A549細胞培養液中的錶達水平高于MRC-5細胞培養液[(31.38±30.58) ng/Lvs (3.10±3.70) ng/L,P＜0.05]；STAT3 mRNA、CEA mRNA在A549細胞中的錶達水平(6.96±3.37,5.68±2.70)均明顯高于MRC-5細胞(1.01±0.19,1.02±0.21)(P＜0.01),且在A549細胞中STAT3和CEA的錶達均呈正相關(r=0.521,P=0.047).結論 STAT3有望成為一種新的診斷肺腺癌的腫瘤標誌物.
목적 탐토신호전도화전록격활인자3( STAT3)적림상응용개치.방법 이용전화학발광면역법검측인폐선암세포A549화인배태성섬유세포MRC-5배양3d적배양액중암배항원(CEA)적표체수평.채용형광정량PCR법검측인폐선암세포A549화인배태성섬유세포MRC-5중STAT3mRNA、CEA mRNA적표체.결과 CEA재A549세포배양액중적표체수평고우MRC-5세포배양액[(31.38±30.58) ng/Lvs (3.10±3.70) ng/L,P＜0.05]；STAT3 mRNA、CEA mRNA재A549세포중적표체수평(6.96±3.37,5.68±2.70)균명현고우MRC-5세포(1.01±0.19,1.02±0.21)(P＜0.01),차재A549세포중STAT3화CEA적표체균정정상관(r=0.521,P=0.047).결론 STAT3유망성위일충신적진단폐선암적종류표지물.
Objective To investigate the clinical significance of signal transducer and activator of trascription 3 (STAT3).Methods The electrochemiluminescence was used to detect the levels of carcinoembryonic antigen (CEA) in medium which cultured lung adenocarcinoma cell A549,human normal lung cell MRC-5 3 days.Relative expression of mRNA of STAT3,CEA in lung adenocarcinoma cell A549 and human normal lung cell MRC-5 was tested by real time PCR.Results The expression of CEA in lung adenocarcinoma cell A549's medium was higher than that in human normal lung cell MRC-5 [(31.38±30.58) ng/L vs (3.10±3.70) ng/L,P ＜0.05].The mRNA levels of STAT3,CEA in A549 cell (6.96±3.37,5.68±2.70) were apparently higher than those in MRC-5 cell (1.01 ± 0.19,1.02±0.21) (P ＜0.01). The mRNA levels of STAT3 had a positive correlation with CEA in lung adenocarcinoma cell A549 ( r =0.521,P =0.047).Conclusions STAT3 can be served as a new marker of lung adenocarcinoma.
