中国临床实用医学
中國臨床實用醫學
중국림상실용의학
CHINA CLINICAL PRACTICAL MEDICINE
2009年
4期
1-4
,共4页
黄辉%王华敏%陈洁%黄湖辉%徐安平
黃輝%王華敏%陳潔%黃湖輝%徐安平
황휘%왕화민%진길%황호휘%서안평
内髓集合管细胞%渗透压%细胞周期%凋亡
內髓集閤管細胞%滲透壓%細胞週期%凋亡
내수집합관세포%삼투압%세포주기%조망
Inner medullary collecting duct cell%Osmotic pressure%Cell cycle%Apoptosis
目的 研究氯化钠梯度渗透压对内髓集合管(Inner medullary collecting duct,IMCD)细胞增殖的影响.方法 采用胶原酶消化和低渗溶解法分离培养SD大鼠原代IMCD细胞.在正常培养基中培养至90%以上细胞融合后,将细胞分为低渗(200 mosmol/kg),等渗(300 mosmoL/kg)和高渗(600 mosmol/kg)培养基组.在12 h和24 h分别应用MTT法(甲基噻唑基四唑)细胞毒性检测活细胞数目和流式细胞仪检测细胞周期和凋亡率,评价细胞的梯度渗透压耐受性.结果 IMCD细胞有很强的渗透压耐受性,IMCD细胞在改变渗透压环境12 h时,高渗环境下细胞凋亡率显著高于低渗和等渗环境(1.1%±0.10% vs 0.565%±0.096%,P＜0.05;0.104%±0.08%,P＜0.05);而24 h时高渗环境下细胞凋亡率略低于低渗环境(P＞0.05),但仍高于等渗环境(P＞0.05).结论 高渗环境可以抑制IMCD细胞的生长,12 h作用较显著;而24 h低渗环境对IMCD细胞的抑制作用增加.
目的 研究氯化鈉梯度滲透壓對內髓集閤管(Inner medullary collecting duct,IMCD)細胞增殖的影響.方法 採用膠原酶消化和低滲溶解法分離培養SD大鼠原代IMCD細胞.在正常培養基中培養至90%以上細胞融閤後,將細胞分為低滲(200 mosmol/kg),等滲(300 mosmoL/kg)和高滲(600 mosmol/kg)培養基組.在12 h和24 h分彆應用MTT法(甲基噻唑基四唑)細胞毒性檢測活細胞數目和流式細胞儀檢測細胞週期和凋亡率,評價細胞的梯度滲透壓耐受性.結果 IMCD細胞有很彊的滲透壓耐受性,IMCD細胞在改變滲透壓環境12 h時,高滲環境下細胞凋亡率顯著高于低滲和等滲環境(1.1%±0.10% vs 0.565%±0.096%,P＜0.05;0.104%±0.08%,P＜0.05);而24 h時高滲環境下細胞凋亡率略低于低滲環境(P＞0.05),但仍高于等滲環境(P＞0.05).結論 高滲環境可以抑製IMCD細胞的生長,12 h作用較顯著;而24 h低滲環境對IMCD細胞的抑製作用增加.
목적 연구록화납제도삼투압대내수집합관(Inner medullary collecting duct,IMCD)세포증식적영향.방법 채용효원매소화화저삼용해법분리배양SD대서원대IMCD세포.재정상배양기중배양지90%이상세포융합후,장세포분위저삼(200 mosmol/kg),등삼(300 mosmoL/kg)화고삼(600 mosmol/kg)배양기조.재12 h화24 h분별응용MTT법(갑기새서기사서)세포독성검측활세포수목화류식세포의검측세포주기화조망솔,평개세포적제도삼투압내수성.결과 IMCD세포유흔강적삼투압내수성,IMCD세포재개변삼투압배경12 h시,고삼배경하세포조망솔현저고우저삼화등삼배경(1.1%±0.10% vs 0.565%±0.096%,P＜0.05;0.104%±0.08%,P＜0.05);이24 h시고삼배경하세포조망솔략저우저삼배경(P＞0.05),단잉고우등삼배경(P＞0.05).결론 고삼배경가이억제IMCD세포적생장,12 h작용교현저;이24 h저삼배경대IMCD세포적억제작용증가.
Objective To investigate the change of the proliferation of inner medullary collecting duct (IMCD) cell under the sodium chloride gradient osmotic pressure circumstance.Methods Collagenase digostion and hypotonic dissolve were used to isolate and culture primary IMCD cell of Sprague-Dawley rat.Cells were divided into hypotonic medium(200 mosmol/kg),isotonic medium(300 mosmol/kg) and hpertonic medium(600 mosmol/kg) groups,after 90% confluence in the normal culture medium.The cellular cytotoxicity was analyzed by MTT assay(methyl thiazolyl tetrazolium) and flow cytometer was used to detect cell cycle and apoptosis ratio.Results IMCD cells were resistant to osmotic pressure change.Cell apoptosis ratio under hypertonic circumstance was significantly higher than hypotonic and isotonic groups after 12 hours treatment(1.1%±0.10% vs 0.565% ±0.096%,0.104%±0.08%,P＜0.05).Cell apoptosis ratio in hypertonic circumstance was slightly lower than hypotonic group (P＞0.05) but higher than isotonic group(P＞0.05) after 24 hours treatment.Conclusion The proliferation of IMCD cell was inhibited under hypertonic circumstance,especially after 12 hours treatment,and the inhibitory effect of hypotonic circumstance increased after 24 hours treatment.
