CAJ | 학술논문

目的通过8-异前列腺素F2α(8-iso-PGF2α)血清浓度的测量来调查系统性硬化症(SSc)的氧化状态,并探讨8-iso-PGF2α与临床特征的相关性.方法通过酶联免疫吸附法检测51例SSc患者及22名年龄与性别匹配的健康对照血清8-iso-PGF2α浓度,并对其中35例SSc患者进行临床评定及实验室检查,以了解各临床特征与8-iso-PGF2α的相关性.另外用循环酶法测定血清同型半胱氨酸(Hcy)浓度,免疫比浊法测定血浆血管性假血友病因子(vWF)活性及血清免疫球蛋白(Ig)浓度,间接免疫荧光法检测血清抗核抗体、抗内皮细胞抗体,免疫印迹法测血清抗Scl-70抗体等,以了解8-is0-PGF2α与上述指标的相关性.结果 SSc患者血清8-iso-PGF2α水平较健康对照升高,其与一氧化碳弥散功能负相关.与高分辨肺CT肺间质纤维化的发生及肾小球叶间动脉阻力指数正相关,而与皮肤、外周血管、心脏、食管受累,疾病活动性、分型及vWF、Hcy、Ig、自身抗体等无相关性.结论升高的氧化应激标志物8-iso-PGF2α与SSc肺间质纤维化及肾血管损害程度相关,进一步说明氧化应激在SSc发病机制中起着重要作用.
목적 통과8-이전렬선소F2α(8-iso-PGF2α)혈청농도적측량래조사계통성경화증(SSc)적양화상태,병탐토8-iso-PGF2α여림상특정적상관성.방법 통과매련면역흡부법검측51례SSc환자급22명년령여성별필배적건강대조혈청8-iso-PGF2α농도,병대기중35례SSc환자진행림상평정급실험실검사,이료해각림상특정여8-iso-PGF2α적상관성.령외용순배매법측정혈청동형반광안산(Hcy)농도,면역비탁법측정혈장혈관성가혈우병인자(vWF)활성급혈청면역구단백(Ig)농도,간접면역형광법검측혈청항핵항체、항내피세포항체,면역인적법측혈청항Scl-70항체등,이료해8-is0-PGF2α여상술지표적상관성.결과 SSc환자혈청8-iso-PGF2α수평교건강대조승고,기여일양화탄미산공능부상관.여고분변폐CT폐간질섬유화적발생급신소구협간동맥조력지수정상관,이여피부、외주혈관、심장、식관수루,질병활동성、분형급vWF、Hcy、Ig、자신항체등무상관성.결론 승고적양화응격표지물8-iso-PGF2α여SSc폐간질섬유화급신혈관손해정도상관,진일보설명양화응격재SSc발병궤제중기착중요작용.
Objective To investigate the oxidative status in systemic sclerosis (SSe) and explore the correlation between oxidative status and clinical features by measuring serum 8-isoprostaglandin-F2α (8-isoPGF2α) concentrations. Methods Serum 8-iso-PGF2α levels were detected by enzyme-linked immunosorbent assay (ELISA) in 51 SSe patients and 22 matched healthy controls. And in some SSc patients, serum homocysteic acid (Hey) concentrations were examined by cyclophorasc assay. Plasma yon Wilebrand factor (vWF) activity and serum immunoglobulin (lg) concentrations were detected by immunoturbidimetry.Antinuclear antibodies (ANA) and anti-endothelial antibodies (AECA) were detected by indirect immunofluorescence. Anti-sc170 antibody was detected by immunoblotting. Thirty-five out of 51 SSc patients were assessed for clinical features and laboratory parameters in order to analyze the correlations between 8-isoPGF2α levels and clinical features. Results Serum 8-iso-PGF2α levels were higher in sclerederma patients than in healthy controls. Values of 8-iso-PGF2α correlated with pulmonary involvement, such as diffusion capacity for carbon monoxide (DLCO) and pulmonary interstitial fibrosis by pulmonary high-resolution computed tomography (PhrCT), and correlated positively with renal vascular damage determined by the resistant index (RI) of renal glomeruli interlobular arteries. There was no correlation between 8-iso-PGF2αconcentrations and skin, peripheral vascular, heart, esophagus involvement and disease activity, diseasepattern, vWF,Hcy, lg, or autoantibedy profiles.Conclusion Increased Jevels of 8-iso-PGF2α, marker of oxidative stress, is correlated with pulmonary fibrosis and the extent of renal vascular damage in SSc and this supports the hypothesis that oxidative stress plays an important role in SSc pathogenesis.