CAJ | 학술논문

目的观察医源性HIV感染者CD8+细胞非细胞毒性HIV抑制反应(CNAR),并比较CNAR与CD4细胞计数的关系.方法免疫磁珠法分离HIV感染者的CD8+细胞,按2:1,1:1,0.5:1和0.25:1的比例,与体外急性感染的CD4+细胞混合培养,测定培养物上清中逆转录酶活性,与阴性对照比较计算病毒抑制率.结果 CNAR活性达到80%病毒抑制率时,CD4<300个/μl组的平均CD8与CD4比例为2.4:1,CD4>300个/μl组的平均CD8与CD4比例为1.3:1,两组间比较差异有统计学意义(P<0.05).结论 HIV感染者的CNAR活性与其CIM细胞计数相关,CD4>300的个体较CD4<300的个体有着更显著的抑制HIV复制的能力.
목적 관찰의원성HIV감염자CD8+세포비세포독성HIV억제반응(CNAR),병비교CNAR여CD4세포계수적관계.방법 면역자주법분리HIV감염자적CD8+세포,안2:1,1:1,0.5:1화0.25:1적비례,여체외급성감염적CD4+세포혼합배양,측정배양물상청중역전록매활성,여음성대조비교계산병독억제솔.결과 CNAR활성체도80%병독억제솔시,CD4<300개/μl조적평균CD8여CD4비례위2.4:1,CD4>300개/μl조적평균CD8여CD4비례위1.3:1,량조간비교차이유통계학의의(P<0.05).결론 HIV감염자적CNAR활성여기CIM세포계수상관,CD4>300적개체교CD4<300적개체유착경현저적억제HIV복제적능력.
Objective To study the CD8+ cell noncytotoxic antiviral response (CNAR) to HIV in nosocomial HIV infected individuals, and reveal the relationship between the CNAR and CD4 cell count. MethodCD8+ cells from HIV-1 sero-positive individuals were separated by immunomagnetic beads and mixed withCD4+ cells at different CD8 CD4 cell input ratios (2:1,1:1,0.5:1 and 0.25:1). Reverse transcriptase (RT) activity of cocultured supernatant was tested and compared with negative control and the suppression rate of HIV-1 replication was measured. Result The average CD8 : CD4 cell input ratios to reach 80% suppression of HIV replication in the group with CD4<300/μl and CD4>300/μl were 2.4:1 and 1.3:1, respectively (P<0.05).Conclusion CNAR activity in HIV infected individuals is associated with CD4<'+> cell count. The ability to suppress HIV replication in subjects with CD4>300 is stronger than those with CD4<300.