CAJ | 학술논문

目的探讨胰岛素样生长因子受体(insulin-like growth factor receptor,1GF-1R和IGF-2R)基因多态性与福建汉族人非小细胞肺癌(non-small-cell lung cancer,NSCLC)易感性的关系.方法采用病例-对照研究,用聚合酶链反应-限制性片段长度多态性和DNA测序法检测福建籍汉族健康人258名和NSCLC患者260例的IGF-IR +1013和IGF-2R+1619两个位点的等位基因分布,探讨不同基因型与NSCLC发病的相关性.结果 (1) IGF-1R +1013(G/A)位点基因型和各等位基因的频率在两组中分布差异有统计学意义(P＜0.05).对于IGF-1R+ 1013(G/A)位点,与GG基因型者相比,GA基因型者NSCLC患病风险增加0.80倍(95％ CI:1.24～2.59,P=0.002),AA基因型者的风险增加2.59倍(95％ CI:1.78～7.26,P=0.000),而等位基因A携带者(GA+AA基因型)患病风险增加0.98倍(95％ CI:1.39～2.83,P=0.000).未发现IGF-2R +1619(G/A)位点各基因型和等位基因的频率分布在两组中差异有统计学意义(P＞0.05).(2)根据病理类型分层分析发现IGF-1R +1013(G/A)变异等位基因A携带者(GA+AA)患肺鳞癌的风险增加2.20倍(95％ CI:1.75～5.84,P=0.000),患肺腺癌的风险增加0.55倍(95％Ch l.00～2.41,P=0.049),患其他类型肺癌的风险增加0.96倍(95％CI:1.10～3.49,P=0.023).未发现两基因多态性与临床分期、淋巴结转移、远处转移有关(P＞0.05).(3)联合分析发现,IGF-1R+1013(G/A)和IGF-2R +1619(G/A)基因联合多态性与NSCLC的患病风险存在相关性(P=0.003).结论 IGF-IR+ 1013(G/A)位点变异等位基因A是肺癌发生的风险因素,IGF-1R+1013(G/A)和IGF-2R+1619(G/A)基因多态性对肺癌的发生有协同作用.
목적 탐토이도소양생장인자수체(insulin-like growth factor receptor,1GF-1R화IGF-2R)기인다태성여복건한족인비소세포폐암(non-small-cell lung cancer,NSCLC)역감성적관계.방법 채용병례-대조연구,용취합매련반응-한제성편단장도다태성화DNA측서법검측복건적한족건강인258명화NSCLC환자260례적IGF-IR +1013화IGF-2R+1619량개위점적등위기인분포,탐토불동기인형여NSCLC발병적상관성.결과 (1) IGF-1R +1013(G/A)위점기인형화각등위기인적빈솔재량조중분포차이유통계학의의(P＜0.05).대우IGF-1R+ 1013(G/A)위점,여GG기인형자상비,GA기인형자NSCLC환병풍험증가0.80배(95％ CI:1.24～2.59,P=0.002),AA기인형자적풍험증가2.59배(95％ CI:1.78～7.26,P=0.000),이등위기인A휴대자(GA+AA기인형)환병풍험증가0.98배(95％ CI:1.39～2.83,P=0.000).미발현IGF-2R +1619(G/A)위점각기인형화등위기인적빈솔분포재량조중차이유통계학의의(P＞0.05).(2)근거병리류형분층분석발현IGF-1R +1013(G/A)변이등위기인A휴대자(GA+AA)환폐린암적풍험증가2.20배(95％ CI:1.75～5.84,P=0.000),환폐선암적풍험증가0.55배(95％Ch l.00～2.41,P=0.049),환기타류형폐암적풍험증가0.96배(95％CI:1.10～3.49,P=0.023).미발현량기인다태성여림상분기、림파결전이、원처전이유관(P＞0.05).(3)연합분석발현,IGF-1R+1013(G/A)화IGF-2R +1619(G/A)기인연합다태성여NSCLC적환병풍험존재상관성(P=0.003).결론 IGF-IR+ 1013(G/A)위점변이등위기인A시폐암발생적풍험인소,IGF-1R+1013(G/A)화IGF-2R+1619(G/A)기인다태성대폐암적발생유협동작용.
[Objective]To assess the association between polymorphisms of insulin-like growth factor receptor (IGF-1R and IGF-2R) and genetic susceptibility and non-small-cell lung cancer (NSCLC).[Methods] A case-control study of 260 patients with NSCLC and 258 cancer-free subjects from Fujian was carried out.Genotypes of polymorphisms of IGF-1R + 1013 and IGF-2R + 1619 were determined by DNA sequencing and polymerase chain reaction-restrictive fragment length polymorphism.[Results] (1) Significant differences in allele frequency and genotypes distribution of IGF-1R + 1013 (G/A) were found between the two groups (P＜0.05).On multivariate analysis after controlling age and gender,compared with GG genotype of the IGF-1R + 1013 (G/A),the risk of lung cancer for individuals with GA genotype was increased by 0.80 times (95 ％CI:1.24-2.59,P=0.002),those with AA genotype was increased by 2.56 times (95％CI:1.78-7.26,P=0.000),and those with the polymorphic A variant (GA or AA) wasincreased by 0.98 times (95 ％ CI:1.39-2.83,P =0.000).No significant differences in genotypic or allelic frequencies of IGF-2R +1619 (G/A) were found between the two groups (P＞ 0.05).(2) After stratification of the clinical status,the IGF-1R + 1013 A allele increased the risk of lung squamous cell carcinoma (OR=3.20,95％CI:1.75-5.84,P=0.000),lung adenocarcinoma (OR=1.55,95％CI:1.00 2.41,P=0.049) and other types of lung cancer (OR=1.96,95％ CI:1.10-3.49,P=0.023),but no association was found between the two SNPs and other clinical features.(3) IGF-1R+1013(G/A) and IGF-2R + 1619 (G/A) polymorphisms showed a synergic effect (P=0.003).[Conclusion] The common 1GF-1R gene polymorphism GI013A may influence the risk of lung cancer.The polymorphisms of IGF-1R +1013 (G/A) and IGF-2R + 1619 (G/A) have synergistic influence on the risk of lung cancer.