CAJ | 학술논문

运片JSSR分子标记的方法,对分布于中国北方7个居群的137个珍珠猪毛菜个体筛选出了15条引物,产生了196条带,其中153条为多态性条带,总的基因多态率为78.06%,多态性在种群水平上较低,平均多态率为45.60%.平均期望杂合度和Shannon信息指数在种间水平上分别为0.1759和0.2811,在居群水平上为0.125l和0.1977,种群内个体间的遗传分化较大,为70.96%,而种群间遗传分化较小,为29.04%,种群间的遗传一致度平均为0.9320.AMOVA分析得到了相似结果.基于Nei's遗传距离的UPGMA聚类分析将7个居群分为3组:德令哈、兰州和其余5个居群(中宁,乌海,民勤,金塔和安西).Mantel检验表明遗传距离与垂直地理分布显著相关(P<O.001).鉴于种群内遗传多样性较低,应当采取有效措施停止人为扰动和砍伐,保护生境,以保存其种质资源.
운편JSSR분자표기적방법,대분포우중국북방7개거군적137개진주저모채개체사선출료15조인물,산생료196조대,기중153조위다태성조대,총적기인다태솔위78.06%,다태성재충군수평상교저,평균다태솔위45.60%.평균기망잡합도화Shannon신식지수재충간수평상분별위0.1759화0.2811,재거군수평상위0.125l화0.1977,충군내개체간적유전분화교대,위70.96%,이충군간유전분화교소,위29.04%,충군간적유전일치도평균위0.9320.AMOVA분석득도료상사결과.기우Nei's유전거리적UPGMA취류분석장7개거군분위3조:덕령합、란주화기여5개거군(중저,오해,민근,금탑화안서).Mantel검험표명유전거리여수직지리분포현저상관(P<O.001).감우충군내유전다양성교저,응당채취유효조시정지인위우동화감벌,보호생경,이보존기충질자원.
Genetic diversity and differentiation among seven populations of Salsola passerina in north-western China were investigated using ISSR markers.15 ISSR primers gave rise to 196 discernible DNA fragments,of which 153(78.06%)were polymorphic.However,the polymorphism at the population level was relatively low,with an average of 45.60%.The mean expected heterozygosity and Shannon,s infor-mation index were 0.1759 and 0.281 1 at the species level,0.125l and 0.1977 at the population level,respectively.Based on Nei's Gst value,a large proportion of genetic variance(70.96%)resided among individuals within populations;however,only 29.04%genetic variance resided among populations.Pair-wise genetic identity values among populations had a mean of 0.932 0.AMOVA analysis also indicated a similar genetic structure.UPGMA cluster analysis based on Nei's genetic distance divided the popula-tions into three main groups:QHDLH,LZXJS and the rest five populations(NXZN,NMWH,MQHLY,JTDX,and AXTS).The mantel test showed that genetic distance was significantly correlated with verti-cal geographical distribution(P<0.001).A means of preserving genetic variation of this endemic species in those regions is to stop deforestation and to protect its suitable habitats in view of the lower genetic diversity within populations.