安徽农业科学
安徽農業科學
안휘농업과학
JOURNAL OF ANHUI AGRICULTURAL SCIENCES
2009年
35期
17350-17352
,共3页
印度橡胶榕%茎尖%离体培养%植株再生
印度橡膠榕%莖尖%離體培養%植株再生
인도상효용%경첨%리체배양%식주재생
Ficus elastica%Shoot tip%In vitro cluture%Plant regeneration
[目的]为印度橡胶榕的工厂化育苗提供理论依据.[方法]以印度橡胶榕"黑金刚"茎尖为材料,以MS为基本培养基添加不同的激素组合进行组织培养,选择最佳的培养基配方.[结果]外植体初代诱导最佳培养基为:MS+6-BA 2.0 mg/L+NAA 0.1 mg/L,诱导率达69.7%;丛生芽增殖最佳培养基为:MS+6-BA 1.0 mg/L+NAA 0.1 mg/L,增殖系数达2.57;最佳生根培养基为:1/2 MS+NAA 0.1 mg/L+IBA 0.1 mg/L,其生根率为100%.[结论]该研究成功建立了印度橡胶榕"黑金刚"的离体再生体系,为其规模化生产提供技术支撑.
[目的]為印度橡膠榕的工廠化育苗提供理論依據.[方法]以印度橡膠榕"黑金剛"莖尖為材料,以MS為基本培養基添加不同的激素組閤進行組織培養,選擇最佳的培養基配方.[結果]外植體初代誘導最佳培養基為:MS+6-BA 2.0 mg/L+NAA 0.1 mg/L,誘導率達69.7%;叢生芽增殖最佳培養基為:MS+6-BA 1.0 mg/L+NAA 0.1 mg/L,增殖繫數達2.57;最佳生根培養基為:1/2 MS+NAA 0.1 mg/L+IBA 0.1 mg/L,其生根率為100%.[結論]該研究成功建立瞭印度橡膠榕"黑金剛"的離體再生體繫,為其規模化生產提供技術支撐.
[목적]위인도상효용적공엄화육묘제공이론의거.[방법]이인도상효용"흑금강"경첨위재료,이MS위기본배양기첨가불동적격소조합진행조직배양,선택최가적배양기배방.[결과]외식체초대유도최가배양기위:MS+6-BA 2.0 mg/L+NAA 0.1 mg/L,유도솔체69.7%;총생아증식최가배양기위:MS+6-BA 1.0 mg/L+NAA 0.1 mg/L,증식계수체2.57;최가생근배양기위:1/2 MS+NAA 0.1 mg/L+IBA 0.1 mg/L,기생근솔위100%.[결론]해연구성공건립료인도상효용"흑금강"적리체재생체계,위기규모화생산제공기술지탱.
[Objective] Provided a theory foundation for industrialized breeding of Ficus elastica. [Method] Using shoot tip of Ficus elastica 'Decora Burgundy' as explants, Using MS as basic medium with different hormone combinations and selected the best medium combination to set up the plant regeneration system of Ficus elastica 'Decora Burgundy'. [Result] The optimum medium for primary induction of axillary buds was MS+6-BA 2.0 mg/L+NAA 0.1 mg/L, the average induction ratio reach 69.7%, the optimum subculture medium for stem multiplication was MS+6-BA 1.0 mg/L+NAA 0.1 mg/L, on which each explants produced 2.57 plantlets on the average. The frequency of rooting reached 100% on 1/2 MS medium supplemented with NAA 0.1 mg/L and 0.1 mg/L IBA. [Conclusion] In vitro culture and plant regeneration system of Ficus elastica 'Decora Burgundy' was established in this study and the establised system provided a technology foundation for large scale production of plantlet.
