CAJ | 학술논문

以玉米骨干自交系‘87-1'、‘综3'、‘137'和‘K12'的幼胚为外植体,研究了影响玉米幼胚再生体系建立的相关因素.结果表明:87-1幼胚在附加500 mg·L~(-1) L-Pro、500 mg·L~(-1) L-Asn和2.5 mg·L~(-1) 2,4-D的N6B5基本培养基上愈伤组织诱导率最高,平均可高达98.1 %;初级愈伤组织在附加500 mg·L~(-1) L-Pro、500 mg·L~(-1) L-Asn和1.25 mg·L~(-1)2,4-D的N6B5继代培养基上胚性愈伤组织诱导率最高,平均可高达65.6 %.在相同诱导、继代培养基和相同培养条件下,4个不同基因型玉米骨干自交系的愈伤组织诱导率无显著性差异,而其胚性愈伤组织诱导率具有显著性差异.继代培养次数对绿苗分化率也产生重要影响,具有显著性差异,其中,1次继代分化率最高;1/2MS培养基附加0.2 mg·L~(-1) KT更有利于分化苗生根.
이옥미골간자교계‘87-1'、‘종3'、‘137'화‘K12'적유배위외식체,연구료영향옥미유배재생체계건립적상관인소.결과표명:87-1유배재부가500 mg·L~(-1) L-Pro、500 mg·L~(-1) L-Asn화2.5 mg·L~(-1) 2,4-D적N6B5기본배양기상유상조직유도솔최고,평균가고체98.1 %;초급유상조직재부가500 mg·L~(-1) L-Pro、500 mg·L~(-1) L-Asn화1.25 mg·L~(-1)2,4-D적N6B5계대배양기상배성유상조직유도솔최고,평균가고체65.6 %.재상동유도、계대배양기화상동배양조건하,4개불동기인형옥미골간자교계적유상조직유도솔무현저성차이,이기배성유상조직유도솔구유현저성차이.계대배양차수대록묘분화솔야산생중요영향,구유현저성차이,기중,1차계대분화솔최고;1/2MS배양기부가0.2 mg·L~(-1) KT경유리우분화묘생근.
Immature embryos of maize skeleton inbred of 87-1, Zong3, 137 and K12 were used as explants to study the regeneration system of maize immature embryos, and the regenerative plants were obtained. The results indicated that the highest frequency of primary callus inducted from immature embryos of maize inbred line 87-1 was obtained on N6B5 medium supplemented with 500 mg·L~(-1) L-pro,500 mg·L~(-1) L-Asn and 2.5 mg·L~(-1) 2,4-D, which was reached to 98.1 %. The highest frequency of embryogenic callus formatted from primary callus were obtained on N6B5 medium supplemented with 500 mg·L~(-1) L-pro, 500 mg·L~(-1) L-Asn and 1.25 mg·L~(-1) 2,4-D, which was reached to 65.6 %. There was no significant differences among the primary callus induction frequency of 4 genotypes under the same culture condition, but the embryogenic callus formation frequencies were significantly different. The effects of subculture times on the frequency of plant regeneration were also significant different, and the frequency of plant regeneration was highest at only once subculture.1/2 MS medium supplemented with 0.2 mg·L~(-1) KT was benefit to the generation of root.