CAJ | 학술논문

目的:建立运动病模型,观察生姜透皮帖对运动病大鼠脑组织几种生化指标的变化,并探讨其对运动病产生治疗作用的机制.方法:雄性Wistar大鼠40只,随机分5组,每组8只,每天按实验方法刺激,实验第4天刺激结束后取大脑和小脑皮质、脑干前庭区.采用组织化学定位方法和图像定量分析方法,检测各组大鼠3部位脑组织中碱性磷酸酶(ALP)、 K~+-Na~+-ATP酶及细胞色素氧化酶(CCO)活性.结果:运动病大鼠3部位脑组织中ALP活性显著升高、Na~+-K~+-ATP酶及CCO活性明显减低.使用生姜透皮帖后运动病大鼠3部位脑组织中ALP活性显著减低、Na~+-K~+-ATP酶及CCO活性明显升高.结论:生姜透皮帖可能使脑血管紧张度降低,改善脑血流的分布,增加脑血流,调整脑细胞内CCO、 ALP、 Na~+-K~+-ATP酶的活性,增强脑细胞的兴奋性,从而保持中枢神经系统的兴奋性,起到抗运动病的作用.
목적:건립운동병모형,관찰생강투피첩대운동병대서뇌조직궤충생화지표적변화,병탐토기대운동병산생치료작용적궤제.방법:웅성Wistar대서40지,수궤분5조,매조8지,매천안실험방법자격,실험제4천자격결속후취대뇌화소뇌피질、뇌간전정구.채용조직화학정위방법화도상정량분석방법,검측각조대서3부위뇌조직중감성린산매(ALP)、 K~+-Na~+-ATP매급세포색소양화매(CCO)활성.결과:운동병대서3부위뇌조직중ALP활성현저승고、Na~+-K~+-ATP매급CCO활성명현감저.사용생강투피첩후운동병대서3부위뇌조직중ALP활성현저감저、Na~+-K~+-ATP매급CCO활성명현승고.결론:생강투피첩가능사뇌혈관긴장도강저,개선뇌혈류적분포,증가뇌혈류,조정뇌세포내CCO、 ALP、 Na~+-K~+-ATP매적활성,증강뇌세포적흥강성,종이보지중추신경계통적흥강성,기도항운동병적작용.
Objective: To observe the biochemical changes in the brain tissue of rat suffering from motion sickness, thereafter to explore its mechanism. Methods: 40 SD rats were randomly and equally divided into five groups, including a normal group and four model groups, of which three groups were treated with different dose of ginger transerm scopolamine. After motion stimulation for four consecutive days, the cerebral cortex, cerebellar cortex and vestibular area of brain stem in rats were removed to detect activity of the alkaline phosphatase, Na~+-K~+ATPase and cytochrome oxidase by means of histochemical techniques and image analysis. Results: Motion sickness induced a significant increase in the alkaline phosphatase activity in the examined rat brain tissues, which was suppressed by ginger transderm scopolamine. The activities of Na~+-K~+ ATPase and cytochrome oxidase were decreased obviously in the rat brain tissues after motion sickness was duplicated, but were improved significantly the by ginger transderm scopolamine. Conclusion: Ginger transderm scopolamine against motion sickness might attribute to its capability for inhibiting vascular tone, improving the distribution of blood flow and increasing cerebral blood flow which could relieve partial blood supply, hippocampal hypoxia and scavenge free radicals.