CAJ | 학술논문

采用油水两相溶液体系,借助于双亲聚合物包覆实现了 CdSe/ZnSe核壳结构量子点自油相到水相的相转移.油水两相中的聚合物包覆与已经报道的均相溶液中聚合物包覆量子点的方法不同,包覆过程在油水两相界面处完成,有效地减少了聚合物缠绕引起的量子点团聚,实现了聚合物埘最子点的无团聚单分散包覆.透射电镜和激光粒度分析仪对聚合物包覆量子点的表征结果表明获得的水溶性量子点具有良好的分散性,均一的水力尺寸.吸收和发射光谱表明聚合物包覆过程对最子点的发射峰佗和峰型没有引起明显的改变,维持了较高的量子产率.通过荧光微区成像技术成功实现了对人IgG蛋白的特异性检测,证实这种方法获得的聚合物包覆量子点具有较好的与生物分子偶联的功能化基团,适合于生物学标记应用.
채용유수량상용액체계,차조우쌍친취합물포복실현료 CdSe/ZnSe핵각결구양자점자유상도수상적상전이.유수량상중적취합물포복여이경보도적균상용액중취합물포복양자점적방법불동,포복과정재유수량상계면처완성,유효지감소료취합물전요인기적양자점단취,실현료취합물시최자점적무단취단분산포복.투사전경화격광립도분석의대취합물포복양자점적표정결과표명획득적수용성양자점구유량호적분산성,균일적수력척촌.흡수화발사광보표명취합물포복과정대최자점적발사봉타화봉형몰유인기명현적개변,유지료교고적양자산솔.통과형광미구성상기술성공실현료대인IgG단백적특이성검측,증실저충방법획득적취합물포복양자점구유교호적여생물분자우련적공능화기단,괄합우생물학표기응용.
In the present work,the CdSe/ZnSe core/shell quantum dots(QDs)were successfully transferred from organic phase to water phase via a two-phase solution system process by surface coating with amphiphilic polymer.Surface coating with amphiphilic polymer is an effective method,which can form stable soluble QDs in water.However,the conventional polymer coating method is performed in homogeneous phase,and it easily induces the aggregation of the QDs attributing to the long chain of enlace of the polymer.It is thus necessary and meaningful to develop surface coating technique for getting monodisperse coating QDs with amphiphilic polymer.In comparison with previously reported coating method,the authors' experiment process is performed in two-phase solution system,and can effectively reduces the possibility of aggregation of the QDs.The resulting hydrophilic CdSe/ZnSe core/shell QDs have long term stability in water,and high quantum yield.The polymer coating process was affirmed by various characterizations.Fourier transform infrared spectra suggest that the octylamine modified polymer was successfully coated on the surface of the CdSe/ZnSe QDs.The transmission electron microscopy suggests that the size and shape of the QDs showed no obvious change before and after the coating process.Dynamic light scattering results indicate that the hydrophilic QDs exhibit narrow hydrodynamic size distribution with the mean hydrodynamic diameters of about 19.7 nm.The luminescence properties of the QDs were investigated with photoluminescence spectra and ultraviolet-visible absorption spectra.This polymer coating process has less effect on luminescence capability.The quantum yield decreased from 43% to 30%.Further,in order to confirm that the polymer capped QDs is biocompatible,the QDs were used for specific detection of the human IgG with fluorescence mapping.The specific molecular recognition capacity of goat anti-human IgG-modified QDs confirms that the polymer coated QDs have compatible functional chemical groups for bioconjugation and are suitable for biological applications.