CAJ | 학술논문

研究了不同因素对板栗壳多酚提取效果的影响,并对不同条件下板栗壳多酚的抗氧化性进行了研究.结果表明,板栗壳多酚的最佳提取条件为:乙醇体积分数59.7%,料液比1:18(g:mL),浸提温度52.6℃,浸提次数3次.在此最优工艺条件下板栗壳多酚提取得率预测值为76.0mg/g,实验值75.9mg/g.板栗壳多酚具有较强的清除自由基能力,在25 ～ 200mg/L范围内,随质量浓度升高其还原能力和清除二苯基苦基肼自由基(DPPH·)、羟基自由基(·OH)、超氧阴离子自由基(O_2~-·)的能力逐渐增强,当200mg/L时其还原能力达0.841,DPPH ·抑制率达89.7%,均高于同质量浓度的2,6-二叔丁基对甲酚(BHT),但低于同质量浓度V_C,对O_2~-·和·OH的清除率分别为93.2%和94.0%,均高于同质量浓度BHT和V_C.
연구료불동인소대판률각다분제취효과적영향,병대불동조건하판률각다분적항양화성진행료연구.결과표명,판률각다분적최가제취조건위:을순체적분수59.7%,료액비1:18(g:mL),침제온도52.6℃,침제차수3차.재차최우공예조건하판률각다분제취득솔예측치위76.0mg/g,실험치75.9mg/g.판률각다분구유교강적청제자유기능력,재25 ～ 200mg/L범위내,수질량농도승고기환원능력화청제이분기고기정자유기(DPPH·)、간기자유기(·OH)、초양음리자자유기(O_2~-·)적능력축점증강,당200mg/L시기환원능력체0.841,DPPH ·억제솔체89.7%,균고우동질량농도적2,6-이숙정기대갑분(BHT),단저우동질량농도V_C,대O_2~-·화·OH적청제솔분별위93.2%화94.0%,균고우동질량농도BHT화V_C.
The effects of different factors on extraction of polyphenol from chestnut shell were studied, and the antioxidation pro-perty of polyphenol of chestnut shell was also studied. The results showed that the optimum extraction conditions were:ethanol volume fraction 59.7%, solid-liquid ratio 1:18(g:mL), extraction temperature 52.6℃ and extract 3 times. The predicted yield of chestnut shell polyphenol is 76.0 mg/g and the test result is 75.9mg/g. Polyphenol in chestnut shell has strong ability to eliminate free radicals. The abilities of reducing and removing DPPH·,·OH, O_2~-·gradually increased with the increase of concentration in the range of 25 to 200mg/L. The ability of reduction reached 0.841, and the rate of inhibition reached 89.7% at 200mg/L, which are higher than those of 2,6-di-tert-butyl-p-cresol (BHT) but lower than those of V_C, at similar mass concentration. The removal rates of O_2~- ·and ·OH are 93.2% and 94.0%, respectively, at concentration of 200mg/L, which are higher than BHT and V_C.