CAJ | 학술논문

目的:筛选鼻咽癌(NPC)转移相关蛋白质,为鼻咽癌防治提供科学依据.方法:采用二维电泳和质谱技术筛选不同转移潜能鼻咽癌细胞系(5-8F和6-10B)的差异表达蛋白质,并应用Western印迹对部分差异蛋白质进行验证；使用siRNA抑制差异蛋白质nm23-H1的表达,分析nm23-H1表达水平对鼻咽癌细胞体外侵袭能力的影响；应用免疫组织化学染色分析nm23-H1表达水平与鼻咽癌临床病理特征和预后的关系.结果:在不同转移潜能鼻咽癌细胞系中鉴定了15个差异表达的蛋白质,并选择性证实3个差异蛋白质；siRNA下调nm23-H1的表达能增强6-10B鼻咽癌细胞的体外侵袭能力；淋巴结转移鼻咽癌组织中nm23-H1的水平显著低于原发性鼻咽癌；nm23-H1的表达水平与鼻咽癌淋巴结和远处转移、临床分期和复发正相关；生存分析显示nm23-H1低表达的鼻咽癌患者预后差；多因素分析显示nm23-H1表达水平是鼻咽癌患者独立的预后因子.结论:nm23-H1是鼻咽癌的转移抑制蛋白和预后因子.
목적:사선비인암(NPC)전이상관단백질,위비인암방치제공과학의거.방법:채용이유전영화질보기술사선불동전이잠능비인암세포계(5-8F화6-10B)적차이표체단백질,병응용Western인적대부분차이단백질진행험증；사용siRNA억제차이단백질nm23-H1적표체,분석nm23-H1표체수평대비인암세포체외침습능력적영향；응용면역조직화학염색분석nm23-H1표체수평여비인암림상병리특정화예후적관계.결과:재불동전이잠능비인암세포계중감정료15개차이표체적단백질,병선택성증실3개차이단백질；siRNA하조nm23-H1적표체능증강6-10B비인암세포적체외침습능력；림파결전이비인암조직중nm23-H1적수평현저저우원발성비인암；nm23-H1적표체수평여비인암림파결화원처전이、림상분기화복발정상관；생존분석현시nm23-H1저표체적비인암환자예후차；다인소분석현시nm23-H1표체수평시비인암환자독립적예후인자.결론:nm23-H1시비인암적전이억제단백화예후인자.
Objective:To identify proteins associated with nasopharyngeal carcinoma (NPC) metastasis,and provide scientific basis for the prevention and cure of NPC.Methods:A two-dimensional gel electrophoresis and mass spectrometry were performed to screen for differential proteins between highly metastatic 5-8F and non-metastatic 6-10B NPC cell lines.Western blot was used to confirm the differential proteins.We used siRNA to inhibit the expression of differential protein nm23-H1 to determine the association of nm23-H1 with NPC in vitro invasive ability.Immunohistochemistry and statistics were used to evaluate the correlation of nm23-H1 expression with clinicopathological features and clinical outcomes in paraffin-embedded archival tissues including 93 cases of primary NPC and 20 cases of cervical lymphonode metastatic NPC (LMNPC).Results:A total of 15 differential proteins in the 2 cell lines were identified by a proteomic approach,and 3 differential proteins were selectively confirmed.Downregulation of nm23-H1 by siRNA significantly increased the in vitro invasive ability of 6-10B.Significant nm23-H1 downregulation was observed in LMNPC compared with primary NPC.nm23-H1 downregulation in primary NPC was positively correlated with lymphonode and distant metastasis,advanced clinical stage and recurrence.Survival curves showed that patients with nm23-H1 downregulation in primary NPC had a poor prognosis.Multivariate analysis confirmed that nm23-H1 expression level in primary NPC was an independent prognostic indicator.Conclusion:nm23-H1 behaves as a metastasis suppressor in NPC,and nm23-H1 downregulation is a biomarker for poor NPC prognosis.