CAJ | 학술논문

目的以人肝细胞癌高、低肺转移潜能细胞株MHCC97-H、MHCC97-L为研究对象,研究趋化因子受体CXCR4在肝细胞癌肺转移过程中的作用和意义.方法 RT-PCR和Western blotting比较MHCC97-H、MHCC97-L细胞株CXCR4 mRNA及蛋白质水平的表达.CXCR4配体SDF-1α及肺提取物趋化实验和抗体抑制实验观察SDF-1α和裸鼠肺组织匀浆液对MHCC97-H的趋化迁移作用.结果趋化因子受体CXCR4在MHCC97-H细胞株表达低于低肺转移潜能细胞株MHCC97-L,蛋白质水平与mRNA水平一致.SDF-1α对MHCC97-H趋化实验表明在1～100 ng/ml浓度范围内均有趋化作用,在浓度为50 ng/L时趋化作用最显著(P＜0.05).肺提取物亦发现对MHCC97-H有趋化作用,作用强于MHCC97-L及DMEM组(P＜0.05).但加入CXCR4抗体后其趋化作用并未明显下调.结论 CXCR4在肝癌组织及细胞表达并可能参与肝癌肺转移,但并非肝癌细胞趋化转移的主要受体分子.
목적 이인간세포암고、저폐전이잠능세포주MHCC97-H、MHCC97-L위연구대상,연구추화인자수체CXCR4재간세포암폐전이과정중적작용화의의.방법 RT-PCR화Western blotting비교MHCC97-H、MHCC97-L세포주CXCR4 mRNA급단백질수평적표체.CXCR4배체SDF-1α급폐제취물추화실험화항체억제실험관찰SDF-1α화라서폐조직균장액대MHCC97-H적추화천이작용.결과 추화인자수체CXCR4재MHCC97-H세포주표체저우저폐전이잠능세포주MHCC97-L,단백질수평여mRNA수평일치.SDF-1α대MHCC97-H추화실험표명재1～100 ng/ml농도범위내균유추화작용,재농도위50 ng/L시추화작용최현저(P＜0.05).폐제취물역발현대MHCC97-H유추화작용,작용강우MHCC97-L급DMEM조(P＜0.05).단가입CXCR4항체후기추화작용병미명현하조.결론 CXCR4재간암조직급세포표체병가능삼여간암폐전이,단병비간암세포추화전이적주요수체분자.
Objective To study the role of chemokine receptor CXCR4 in lung metastasis of hepatocellular carcinoma(HCC)through human HCC cell line MHCC97-H and MHCC97-L with different lung metastasis potentials.Methods The mRNA and protein expression of CXCR4 between MHCC97-H and MHCC97-L by were determined bv RT-PCR and Western blotting respectively.Chemotaxis and antibody inhibition assays were used to observe the chemotaxis effects of SDF-1α,specific ligant of CXCR4, and lung crude extraction from nude mice on MHCC97-H.Results Chemokine receptor CXCR4 in MHCC97-H was expressed lower than that in MHCC97-L,and protein level was in consistent with the mRNA level.MHCC97-H could respond to SDF-1 αbetween 1 ng/ml to100 ng/ml,particularly at 50 ng/L(P＜0.05).Lung extraction could have chemotaxis to MHCC97-H,compared with other groups(P＜0.05).However,the chemotaxis effect decreased little when being added with CXCR4 antibody.Conclusion Chemokine receptor CXCR4 may participate in the lung metastasis of HCC but is not the major chemokine receptor involved.