CAJ | 학술논문

目的探讨胰腺癌肿瘤干细胞对抗肿瘤药物的敏感性.方法 FACS技术分选人胰腺癌PANC-1细胞；RT-PCR技术检测分选PANC-1细胞中CD133、ABCG2、Notch1的表达情况；MTT法检测分选细胞对抗肿瘤药物5-氟尿嘧啶和吉西他滨的耐药性.建立分选细胞的移植瘤模型,随机分为吉西他滨治疗组(n=3)和对照组(n=3),观察肿瘤生长情况,作CD133免疫组化染色.结果PANC-1细胞中含有SP亚群.SP细胞CD133、ABCG2、Notch1的mRNA的表达明显上调,non-SP细胞的表达量显著低于前者.在吉西他滨的干预下,SP细胞和non-SP细胞的OD值差异有统计学意义.而5-氟尿嘧啶的干预(10 μg/ml和100 μg/ml)则没有显著差异.移植肿瘤治疗组中CD133阳性细胞明显多于对照组(P=0.001).结论胰腺癌中存在SP亚群细胞.胰腺癌PANC-1的成瘤能力是由其中的SP亚群细胞决定的,而并非所有胰腺癌PANC-1细胞.胰腺癌肿瘤干细胞对抗肿瘤药吉西他滨具有较高耐药性.
목적 탐토이선암종류간세포대항종류약물적민감성.방법 FACS기술분선인이선암PANC-1세포；RT-PCR기술검측분선PANC-1세포중CD133、ABCG2、Notch1적표체정황；MTT법검측분선세포대항종류약물5-불뇨밀정화길서타빈적내약성.건립분선세포적이식류모형,수궤분위길서타빈치료조(n=3)화대조조(n=3),관찰종류생장정황,작CD133면역조화염색.결과PANC-1세포중함유SP아군.SP세포CD133、ABCG2、Notch1적mRNA적표체명현상조,non-SP세포적표체량현저저우전자.재길서타빈적간예하,SP세포화non-SP세포적OD치차이유통계학의의.이5-불뇨밀정적간예(10 μg/ml화100 μg/ml)칙몰유현저차이.이식종류치료조중CD133양성세포명현다우대조조(P=0.001).결론이선암중존재SP아군세포.이선암PANC-1적성류능력시유기중적SP아군세포결정적,이병비소유이선암PANC-1세포.이선암종류간세포대항종류약길서타빈구유교고내약성.
Objective To explore the sensitivity of cancer stem cells to chemotherapy in human pancreatic carcinoma.Methods PANC-1 ceils were cultured in an incubator filled with 5％ CO2 at a temperature of 37℃,and were labeled with Hoechst 33342.The SP analysis and sorting were performed using a FACSVantage SE.RT-PCR was performed to detect the expression of human CD133 ABCG2 and Notch1.SP and non-SP cells from the PANC-1 cell line were treated with 5-fluorouracil (5-FU; 1,10,or 100 μg/ml) or gemcitabine (10,100,or 1000μg/ml),and the cell viability was determined using the MTT assay.The sensitivity of sorted tumor cells to chemotherapeutics was determined in NOD/SCID mice model.Results SP cells were found to have higher drug-resistance both in vivo and in vitro and higher levels of mRNA expression for CD133,ABCG2 and Notch1,when compared to non-SP ceils.The xenografted tumors derived from injected SP cells and treated with gemcitabine had more CD133± cells than the untreated group.Conclusions The SP of PANC-1 pancreatic carcinoma cells are enriched with highly gemcitabine-resistant CSCs and determine the carcinogenesis of the PANC-1 pancreatic carcinoma cells.