中华预防医学杂志
中華預防醫學雜誌
중화예방의학잡지
CHINESE JOURNAL OF
2009年
1期
41-44
,共4页
王玉林%刘于飞%蒋力云%柳洋%杨智聪%郝爱华%伍业健%李海麟%李铁钢%王鸣
王玉林%劉于飛%蔣力雲%柳洋%楊智聰%郝愛華%伍業健%李海麟%李鐵鋼%王鳴
왕옥림%류우비%장력운%류양%양지총%학애화%오업건%리해린%리철강%왕명
禽流感%接触者追踪%疾病传播%危险性评估
禽流感%接觸者追蹤%疾病傳播%危險性評估
금류감%접촉자추종%질병전파%위험성평고
Avian influenza%Contact tracing%Disease transmission%Risk assessment
目的 评估动物禽流感疫情暴发后人群感染的风险,探讨禽流感传播的可能性.方法 采用现场流行病学调查、分子流行病学、血清学研究及应急监测方法 ,对病、死禽的所有密切接触者进行医学观察;采用红细胞凝集抑制实验、实时荧光逆转录-聚合酶链式反应(RT-PCR)、基因测序方法 ,检测全部密切接触者的血清抗体,采集4个疫点环境标本检测禽流感H5核酸.结果 检测4个疫点环境标本22份,H5核酸阳性1份,序列分析与广州市2006年人禽流感病毒株A/China/GD01/2006(H5N1)的同源性为95.9%;检测疫区及周边2个农贸市场活禽交易场所环境标本62份,H5核酸均阴性;采集密切接触者的血样68份、咽拭子68份,禽流感H9抗体阳性6份,H5抗体、H5核酸均阴性,医学观察7 d,未发现禽流感感染者;应急监测区报告流感样患者337例,经排查未发现可疑禽流感患者.结论 此起水禽H5N1暴发未造成扩散,也未出现人感染病例,表明此次疫情的禽流感病毒H5N1对人的传播能力尚不强,引起人群感染的风险较低.
目的 評估動物禽流感疫情暴髮後人群感染的風險,探討禽流感傳播的可能性.方法 採用現場流行病學調查、分子流行病學、血清學研究及應急鑑測方法 ,對病、死禽的所有密切接觸者進行醫學觀察;採用紅細胞凝集抑製實驗、實時熒光逆轉錄-聚閤酶鏈式反應(RT-PCR)、基因測序方法 ,檢測全部密切接觸者的血清抗體,採集4箇疫點環境標本檢測禽流感H5覈痠.結果 檢測4箇疫點環境標本22份,H5覈痠暘性1份,序列分析與廣州市2006年人禽流感病毒株A/China/GD01/2006(H5N1)的同源性為95.9%;檢測疫區及週邊2箇農貿市場活禽交易場所環境標本62份,H5覈痠均陰性;採集密切接觸者的血樣68份、嚥拭子68份,禽流感H9抗體暘性6份,H5抗體、H5覈痠均陰性,醫學觀察7 d,未髮現禽流感感染者;應急鑑測區報告流感樣患者337例,經排查未髮現可疑禽流感患者.結論 此起水禽H5N1暴髮未造成擴散,也未齣現人感染病例,錶明此次疫情的禽流感病毒H5N1對人的傳播能力尚不彊,引起人群感染的風險較低.
목적 평고동물금류감역정폭발후인군감염적풍험,탐토금류감전파적가능성.방법 채용현장류행병학조사、분자류행병학、혈청학연구급응급감측방법 ,대병、사금적소유밀절접촉자진행의학관찰;채용홍세포응집억제실험、실시형광역전록-취합매련식반응(RT-PCR)、기인측서방법 ,검측전부밀절접촉자적혈청항체,채집4개역점배경표본검측금류감H5핵산.결과 검측4개역점배경표본22빈,H5핵산양성1빈,서렬분석여엄주시2006년인금류감병독주A/China/GD01/2006(H5N1)적동원성위95.9%;검측역구급주변2개농무시장활금교역장소배경표본62빈,H5핵산균음성;채집밀절접촉자적혈양68빈、인식자68빈,금류감H9항체양성6빈,H5항체、H5핵산균음성,의학관찰7 d,미발현금류감감염자;응급감측구보고류감양환자337례,경배사미발현가의금류감환자.결론 차기수금H5N1폭발미조성확산,야미출현인감염병례,표명차차역정적금류감병독H5N1대인적전파능력상불강,인기인군감염적풍험교저.
Objective To evaluate the risk of human infection after the outbreak of avian influenza H5N1 in animals.and probe the possibility for virus transmission.Methods By means of field epidemiological study,molecular epidemiology,serology and emergency surveillance,persons who had ever closely contacted with sick or dead poultry were observed.While,the RT-PCR and gene sequencing method were used to detect H5 nucleic acid from environmental swabs from 4 epidemic spots,and hemagglutination inhibition assay was also used to detect H5 antibody.Results of 22 environmental swabs detected from 4 epidemic spots,one was positive for H5 nucleic acid,and the homogeneity was 95.9% as compared with H5N1 virus A/China,/GD01/2006 (H5N1) found in Guangzhou in 2006 by gene sequence analysis.62 environmental swabs from live poultry stalls of food markets near epidemic spot were detected negative.Six of 68 blood samples of contacts were positive for H9 antibody,and all were negative for H5 antibodv.68 throat swabs of contacts were detected negative for H5 nucleic acid.No close contact was found abnormal after 7 days medical observation.337 influenza-like cases were reported in emergency surveillance,and no suspicious case was found.Conclusion The current outbreak of H5N1 avian influenza in water fowls has not yet caused further transmission,and human avian influenza case has not been observed.It indicates that the ability of H5N1 virus to transmit to human is not strong yet,and the risk of human infection for H5N1 is still low.
