CAJ | 학술논문

目的通过研究芳维A酸氨丁三醇(AT)诱导永生化人类角质形成细胞(HaCat)细胞后的基因表达差异,初步探讨第三代维A酸治疗银屑病的机制.方法应用基因芯片技术,检测浓度为10-6 mol/L的AT诱导HaCat细胞24 h后,相对于空白组细胞基因表达谱的改变,并用实时PCR对芯片结果进行验证.结果在4000条人类全长cDNA中,有580条的表达量差异达到2倍以上,其中注释完整的基因有253条(143条表达量增加,110条表达量减少).异常表达的基因主要参与细胞周期及细胞免疫两个过程.结论利用基因芯片技术可同时高通量地研究第3代维A酸对HaCat细胞基因表达的影响,并且发现该类药物能够通过调控细胞周期等多种途径发挥其药理学作用.
목적 통과연구방유A산안정삼순(AT)유도영생화인류각질형성세포(HaCat)세포후적기인표체차이,초보탐토제삼대유A산치료은설병적궤제.방법 응용기인심편기술,검측농도위10-6 mol/L적AT유도HaCat세포24 h후,상대우공백조세포기인표체보적개변,병용실시PCR대심편결과진행험증.결과 재4000조인류전장cDNA중,유580조적표체량차이체도2배이상,기중주석완정적기인유253조(143조표체량증가,110조표체량감소).이상표체적기인주요삼여세포주기급세포면역량개과정.결론 이용기인심편기술가동시고통량지연구제3대유A산대HaCat세포기인표체적영향,병차발현해류약물능구통과조공세포주기등다충도경발휘기약이학작용.
Objective To investigate the gene expression pattern of human keratinocytes (KCs) after arotinoid trometamol (AT) treatment.Methods Human keratinocytes (KCs) of the line HaCat were cultured.AT(10-6 mol/L) was added in the culture fluid for 24 h.A gene chip containing 4000 cDNA clones was used to identify the differentially expressed genes in the HaCat cells,and then the results were verified by real-time PCR.Results Gene chip hybridization showed that 580 of the 4000 full-length human cDNA exhibited differential expression (≥2-fold);253 genes with complete annotation were identified,including 143 genes up-regulated and 110 genes down-regulated.The differentially expressed genes mainly participated into the processes of cell cycle and immunology.Conclusion Microarry technology helps study the third generation of retinoid acid induced gene expression pattern in human KCs in a high through-put way.AT can be used to treat psoriasis by regulating some processes,such as the cell cycle,inflammation,etc.