中华消化杂志
中華消化雜誌
중화소화잡지
Chinese Journal of Digestion
2012年
7期
441-444
,共4页
戴迟兵%刘娜%钱伟%侯晓华
戴遲兵%劉娜%錢偉%侯曉華
대지병%류나%전위%후효화
四磨汤%糖尿病%幽门窦%胃促生长素%降钙素基因相关肽%大鼠,Sprague-Dawley
四磨湯%糖尿病%幽門竇%胃促生長素%降鈣素基因相關肽%大鼠,Sprague-Dawley
사마탕%당뇨병%유문두%위촉생장소%강개소기인상관태%대서,Sprague-Dawley
Simo decoction%Diabetes mellitus%Pyloric antrum%Ghrelin%Calcitonin generelated peptide%Rats,Sprague-Dawley
目的 研究四磨汤对糖尿病大鼠降钙素基因相关肽( CGRP)及饥饿素的影响.方法 48只SD大鼠分为健康对照组、糖尿病组、糖尿病四磨汤低剂量治疗组、糖尿病四磨汤中剂量治疗组、糖尿病四磨汤高剂量治疗组及糖尿病中剂量预防组,每组8只.予糖尿病组及各干预组大鼠1％链脲佐菌素腹腔注射造模,健康对照组仅予柠檬酸母液注射.造模成功后8周,各干预组大鼠予低(0.15 ml·kg-1·d-1)、中(1.5ml·kg-1·d-1)、高(3.0 ml·kg-1·d-1)剂量四磨汤灌胃2周,对照组及糖尿病组予等体积0.9％NaCl溶液灌胃.预防组于造模成功后即开始予1.5 ml·kg-1·d-1四磨汤灌胃10周.药物干预结束后处死大鼠,取胃窦组织行实时PCR检测饥饿素、CGRP的表达.数据处理采用单因素方差分析和t检验.结果 糖尿病大鼠胃窦饥饿素基因水平较对照组无明显变化,但低、中剂量四磨汤干预后饥饿素表达水平高于糖尿病组(1.45±0.34、1.87±0.68比0.87±0.28,Dunnett's T3检验,P＜0.05),高剂量四磨汤干预对糖尿病大鼠饥饿素的表达无影响,四磨汤中剂量预防或治疗对糖尿病大鼠饥饿素的影响差异无统计学意义；糖尿病大鼠胃窦CGRP基因水平明显高于对照组(4.61±1.67比1.06±0.40,t=5.843,P＜0.01),低剂量四磨汤干预对糖尿病大鼠CGRP表达无影响,四磨汤中、高剂量干预可下调糖尿病大鼠CGRP的表达,四磨汤中剂量预防或治疗对糖尿病大鼠CGRP表达的影响差异无统计学意义.结论 四磨汤可促进糖尿病大鼠胃窦饥饿素的基因表达.糖尿病大鼠胃窦CGRP基因表达显著增加,但四磨汤可下凋糖尿病大鼠胃窦CGRP的基因表达水平.
目的 研究四磨湯對糖尿病大鼠降鈣素基因相關肽( CGRP)及饑餓素的影響.方法 48隻SD大鼠分為健康對照組、糖尿病組、糖尿病四磨湯低劑量治療組、糖尿病四磨湯中劑量治療組、糖尿病四磨湯高劑量治療組及糖尿病中劑量預防組,每組8隻.予糖尿病組及各榦預組大鼠1％鏈脲佐菌素腹腔註射造模,健康對照組僅予檸檬痠母液註射.造模成功後8週,各榦預組大鼠予低(0.15 ml·kg-1·d-1)、中(1.5ml·kg-1·d-1)、高(3.0 ml·kg-1·d-1)劑量四磨湯灌胃2週,對照組及糖尿病組予等體積0.9％NaCl溶液灌胃.預防組于造模成功後即開始予1.5 ml·kg-1·d-1四磨湯灌胃10週.藥物榦預結束後處死大鼠,取胃竇組織行實時PCR檢測饑餓素、CGRP的錶達.數據處理採用單因素方差分析和t檢驗.結果 糖尿病大鼠胃竇饑餓素基因水平較對照組無明顯變化,但低、中劑量四磨湯榦預後饑餓素錶達水平高于糖尿病組(1.45±0.34、1.87±0.68比0.87±0.28,Dunnett's T3檢驗,P＜0.05),高劑量四磨湯榦預對糖尿病大鼠饑餓素的錶達無影響,四磨湯中劑量預防或治療對糖尿病大鼠饑餓素的影響差異無統計學意義；糖尿病大鼠胃竇CGRP基因水平明顯高于對照組(4.61±1.67比1.06±0.40,t=5.843,P＜0.01),低劑量四磨湯榦預對糖尿病大鼠CGRP錶達無影響,四磨湯中、高劑量榦預可下調糖尿病大鼠CGRP的錶達,四磨湯中劑量預防或治療對糖尿病大鼠CGRP錶達的影響差異無統計學意義.結論 四磨湯可促進糖尿病大鼠胃竇饑餓素的基因錶達.糖尿病大鼠胃竇CGRP基因錶達顯著增加,但四磨湯可下凋糖尿病大鼠胃竇CGRP的基因錶達水平.
목적 연구사마탕대당뇨병대서강개소기인상관태( CGRP)급기아소적영향.방법 48지SD대서분위건강대조조、당뇨병조、당뇨병사마탕저제량치료조、당뇨병사마탕중제량치료조、당뇨병사마탕고제량치료조급당뇨병중제량예방조,매조8지.여당뇨병조급각간예조대서1％련뇨좌균소복강주사조모,건강대조조부여저몽산모액주사.조모성공후8주,각간예조대서여저(0.15 ml·kg-1·d-1)、중(1.5ml·kg-1·d-1)、고(3.0 ml·kg-1·d-1)제량사마탕관위2주,대조조급당뇨병조여등체적0.9％NaCl용액관위.예방조우조모성공후즉개시여1.5 ml·kg-1·d-1사마탕관위10주.약물간예결속후처사대서,취위두조직행실시PCR검측기아소、CGRP적표체.수거처리채용단인소방차분석화t검험.결과 당뇨병대서위두기아소기인수평교대조조무명현변화,단저、중제량사마탕간예후기아소표체수평고우당뇨병조(1.45±0.34、1.87±0.68비0.87±0.28,Dunnett's T3검험,P＜0.05),고제량사마탕간예대당뇨병대서기아소적표체무영향,사마탕중제량예방혹치료대당뇨병대서기아소적영향차이무통계학의의；당뇨병대서위두CGRP기인수평명현고우대조조(4.61±1.67비1.06±0.40,t=5.843,P＜0.01),저제량사마탕간예대당뇨병대서CGRP표체무영향,사마탕중、고제량간예가하조당뇨병대서CGRP적표체,사마탕중제량예방혹치료대당뇨병대서CGRP표체적영향차이무통계학의의.결론 사마탕가촉진당뇨병대서위두기아소적기인표체.당뇨병대서위두CGRP기인표체현저증가,단사마탕가하조당뇨병대서위두CGRP적기인표체수평.
Objective To investigate the effects of Simo decoction on ghrelin and calcitonin gene-related peptide (CGRP) in diabetic rats.Methods A total of 48 SD rats were divided into healthy control group,diabetes group (DM),DM treated with low dose Simo decoction group (DMTL),DM treated with medium dose Simo decoction group (DM-TM),DM treated with high dose Simo decoction group (DM-TH) and DM prevented with medium dose Simo decoction group (DMPM) ; there were eight rats in each group.The rats of DM group and other intervention groups were intraperitoneally injected with 1％ streptozotoein (STZ) to establish the model.The rats of healthy control group were injected with solvent.At eight weeks after the model successfully established,the rats of each intervention group were gavaged with low dose of Simo decoction (0.15 ml · kg-1 · d-1 ),medium dose of Simo decoction (1.5 ml · kg-1 · d-1 ) and high dose of Simo decoction (3.0 ml · kg-1 ·d-1 ) for two weeks; the rats of control group and DM groups were gavaged with equal volume of 0.9％NaCl.The rats of DM-PM group were gavaged with Simo decoction at 1.5 ml · kg-1 · d-1 for 10 weeks after the model successfully established.Rats were sacrificed at the end of drug intervention and gastric antrum tissues were dissociated for detecting the expression of ghrelin and CGRP by real time polymerase chain reaction (PCR).The data were analyzed by one way ANOVA and t test.Results There was no significant difference in ghrelin expression at mRNA level in gastric antrum tissues between diabetic rats and control group.However after intervened by low dose and medium dose of Simo decoction,the ghrelin expression increased (1.45 ± 0.34,1.87 ± 0.68 compared with 0.87 ±0.28,Dunnett's T3 test,P＜0.05).There was no effects of high dose Simo decoction on the expression of ghrelin in diabetic rats.There was no significant difference in ghrelin between DM-TM and DM-PM.The expression of CGRP at mRNA level in gastric antrum tissues of diabetic rats was significantly higher than that of control group (4.61 ± 1.67 compared with 1.06 ± 0.40,t =5.843,P＜0.01).There was no effects of low dose Simo decoction on CGRP expression of diabetic rats.The expression of CGRP of diabetic rats was down-regulated by medium and high dose of Simo decoction intervention.There was no significant difference in CGRP expression between DM-TM and DM-PM.Conclusions Simo decoction can promote ghrelin expression in gastric antrum of diabetic rats.The expression of CGRP in gastric antrum of diabetic rats significantly increased,but Simo decoction may down-regulate the expression of CGRP in gastric antrum of diabetic rats.
