CAJ | 학술논문

目的:研究腹腔注射多巴胺对鸡剥夺性近视眼的抑制作用.方法:在鸡出生后的7d,右眼遮盖黑色眼罩以构建剥夺性近视眼模型,左眼开放作为对照.实验动物分为对照组(n=2)和治疗组(n=3),对照组和治疗组的腹腔分别注射0.25mL生理盐水或0.25mL 多巴胺溶液(20μg多巴胺),1次/d,共12d.取出动物眼球测量眼球的质量,赤道部直径和眼轴长度.选择具有角膜和视神经的眼球组织学切片,计算巩膜前部、赤道部和后极部分裂软骨细胞占总软骨细胞的百分率.结果:多巴胺对鸡剥夺性近视眼的的发展有抑制作用;分裂巩膜软骨细胞的百分率在剥夺性近视眼增高,尤其在后极部;分裂巩膜软骨细胞的百分率在对照组的遮盖眼和治疗组的遮盖眼之间无差别.结论:本实验的结果与目前认为多巴胺对近视眼发展有抑制作用的观点相一致,多巴胺可能是抑制近视眼发展的重要因子.
목적:연구복강주사다파알대계박탈성근시안적억제작용.방법:재계출생후적7d,우안차개흑색안조이구건박탈성근시안모형,좌안개방작위대조.실험동물분위대조조(n=2)화치료조(n=3),대조조화치료조적복강분별주사0.25mL생리염수혹0.25mL 다파알용액(20μg다파알),1차/d,공12d.취출동물안구측량안구적질량,적도부직경화안축장도.선택구유각막화시신경적안구조직학절편,계산공막전부、적도부화후겁부분렬연골세포점총연골세포적백분솔.결과:다파알대계박탈성근시안적적발전유억제작용;분렬공막연골세포적백분솔재박탈성근시안증고,우기재후겁부;분렬공막연골세포적백분솔재대조조적차개안화치료조적차개안지간무차별.결론:본실험적결과여목전인위다파알대근시안발전유억제작용적관점상일치,다파알가능시억제근시안발전적중요인자.
AIM: To evaluate the effect of intraperitoneal injection of dopamine on the development of deprivation myopia in a chick model.METHODS: On the 7th postnatal day, the chicks′right eyes were covered with black goggles and left eyes acted as controls. Chicks were divided into two groups, two controls and three treated. Saline control (0.25mL) and dopamine solution (0.25mL, 20μg dopamine) was administrated into the control and the treated group respectively, once a day for 12 days. Eye weight was measured with a digital balance. Equatorial diameter and axial length were measured with calipers. Six high- power fields in those sections with cornea and optic nerve were chosen,including two of anterior sclera, two of equatorial sclera and two of posterior pole sclera. In each field, the percentage of the mitotic chondrocyte in total chondrocyte was calculated.RESULTS: Dopamine reduced the severity of deprivation myopia. The percentage of mitotic chondrocyte in goggled eye increased, particularly on the posterior pole of the sclera. As for the percentage of mitotic chondrocyte, there was no significant difference between control goggled eyes and treated goggled eyes.CONCLUSION: The results of the present study is in good agreement with the current understanding that dopamine has a beneficial effect on myopia. Dopamine may be a crucial factor in suppressing the development of myopia.