航天医学与医学工程
航天醫學與醫學工程
항천의학여의학공정
SPACE MEDICINE & MEDICAL ENGINEERING
2004年
5期
313-317
,共5页
石宏志%高南南%李勇枝%余竞光%范全春%白桂娥
石宏誌%高南南%李勇枝%餘競光%範全春%白桂娥
석굉지%고남남%리용지%여경광%범전춘%백계아
泽兰%有效部位%动静脉旁路%血小板%血栓形成%血小板凝集%体外
澤蘭%有效部位%動靜脈徬路%血小闆%血栓形成%血小闆凝集%體外
택란%유효부위%동정맥방로%혈소판%혈전형성%혈소판응집%체외
lycopus lucidus%active fraction%artery-vein by-pass%blood platelets%thrombosis%platelet aggregation%in vitro
目的研究泽兰有效部位L.F04对血小板聚集和血栓形成的影响,以探讨其活血化瘀作用机理.方法 用高分子右旋糖酐静脉推注造成大鼠血瘀证动物模型,观察泽兰L.F04对ADP诱导的大鼠体内血小板聚集以及体内动静脉旁路血栓、体外旋转环内血栓形成的影响.结果 L.F04 0.408 g/kg、0.204 g/kg对模型组大鼠ADP诱导的体内血小板最大聚集率明显增加皆有显著的抑制,且呈剂量依赖关系;与对照组相比,血瘀模型大鼠体外血栓重量明显增加,长度仅有增加趋势,L.F04 0.408 g/kg 、0.204 g/kg皆有抗血栓形成作用,L.F04 0.408 g/kg对血栓干重、湿重的减轻尤为明显;L.F04 0.408 g/kg 、0.204 g/kg对实验性动静脉旁路血栓形成均有明显的抑制作用,抑制率分别为27.41 %、27.14 %.结论 泽兰L.F04可显著抑制血小板聚集及体内、外血栓形成.
目的研究澤蘭有效部位L.F04對血小闆聚集和血栓形成的影響,以探討其活血化瘀作用機理.方法 用高分子右鏇糖酐靜脈推註造成大鼠血瘀證動物模型,觀察澤蘭L.F04對ADP誘導的大鼠體內血小闆聚集以及體內動靜脈徬路血栓、體外鏇轉環內血栓形成的影響.結果 L.F04 0.408 g/kg、0.204 g/kg對模型組大鼠ADP誘導的體內血小闆最大聚集率明顯增加皆有顯著的抑製,且呈劑量依賴關繫;與對照組相比,血瘀模型大鼠體外血栓重量明顯增加,長度僅有增加趨勢,L.F04 0.408 g/kg 、0.204 g/kg皆有抗血栓形成作用,L.F04 0.408 g/kg對血栓榦重、濕重的減輕尤為明顯;L.F04 0.408 g/kg 、0.204 g/kg對實驗性動靜脈徬路血栓形成均有明顯的抑製作用,抑製率分彆為27.41 %、27.14 %.結論 澤蘭L.F04可顯著抑製血小闆聚集及體內、外血栓形成.
목적연구택란유효부위L.F04대혈소판취집화혈전형성적영향,이탐토기활혈화어작용궤리.방법 용고분자우선당항정맥추주조성대서혈어증동물모형,관찰택란L.F04대ADP유도적대서체내혈소판취집이급체내동정맥방로혈전、체외선전배내혈전형성적영향.결과 L.F04 0.408 g/kg、0.204 g/kg대모형조대서ADP유도적체내혈소판최대취집솔명현증가개유현저적억제,차정제량의뢰관계;여대조조상비,혈어모형대서체외혈전중량명현증가,장도부유증가추세,L.F04 0.408 g/kg 、0.204 g/kg개유항혈전형성작용,L.F04 0.408 g/kg대혈전간중、습중적감경우위명현;L.F04 0.408 g/kg 、0.204 g/kg대실험성동정맥방로혈전형성균유명현적억제작용,억제솔분별위27.41 %、27.14 %.결론 택란L.F04가현저억제혈소판취집급체내、외혈전형성.
Objective To study the effect of active fractions from Lycopus lucidus (L.F04) on platelet aggregation and thrombus formation so as to investigate its mechanism of promoting blood circulation and removing blood stasis. Method The effects of Lycopus lucidus L.F04 on platelet aggregation induced by ADP in vivo, and thrombosis of artery-vein by-pass and thrombus formed in circuitous loop in vitro were examined using the blood stasis animal model of rats made by injecting high molecular weight dextran (HMWD). Result The increase of maximum platelet aggregation rate induced by ADP in vivo in HMWD model was evidently inhibited with 0.408 g/kg or 0.204 g/kg of L.F04, and the effect showed a trend of concentration-dependence. As compared with the control, the thrombus weight in rat model of blood stasis increased clearly and its length showed only a trend of increase. Both 0.408 g/kg and 0.204 g/kg of L.F04 had the effect of resisting the thrombus formation, while 0.408 g/kg showed better effects of reducing the thrombus dry weight and wet weight. Both 0.408 g/kg and 0.204 g/kg of L.F04 could inhibit the thrombosis in artery-vein by-pass; the inhibition rates were 27.41 % and 27.14 % respectively. Conclusion Lycopus lucidus L.F04 could significantly inhibit platelet aggregation and thrombus formation.
