CAJ | 학술논문

为了探讨强制运动对成年大鼠海马齿状回(dentate gyrus,DG)神经发生的影响,强制大鼠在马达驱动的转轮中跑步,用5-溴-2-脱氧尿苷(5-bromo-2-deoxyuridine,BrdU)标记增殖细胞,巢蛋白(neuroepthelial stem cell protein,nestin)标记神经干细胞/前体细胞,然后用免疫细胞化学技术检测大鼠DG中BrdU及nestin阳性细胞.为了解强制运动后DG增殖细胞的功能意义,采用Y-迷宫检测大鼠的学习能力.结果表明,强制运动组DG中BrdU及nestin阳性细胞数均明显多于对照组(P＜0.05);强制运动对DG神经发生的效应有强度依赖性.Y-迷宫检测结果显示,强制运动能明显改善大鼠的学习能力.结果提示,在转轮中进行强制跑步能促进成年大鼠DG的神经发生,并改善学习能力.
위료탐토강제운동대성년대서해마치상회(dentate gyrus,DG)신경발생적영향,강제대서재마체구동적전륜중포보,용5-추-2-탈양뇨감(5-bromo-2-deoxyuridine,BrdU)표기증식세포,소단백(neuroepthelial stem cell protein,nestin)표기신경간세포/전체세포,연후용면역세포화학기술검측대서DG중BrdU급nestin양성세포.위료해강제운동후DG증식세포적공능의의,채용Y-미궁검측대서적학습능력.결과표명,강제운동조DG중BrdU급nestin양성세포수균명현다우대조조(P＜0.05);강제운동대DG신경발생적효응유강도의뢰성.Y-미궁검측결과현시,강제운동능명현개선대서적학습능력.결과제시,재전륜중진행강제포보능촉진성년대서DG적신경발생,병개선학습능력.
To investigate the effect of forced running in motor-driven wheel on neurogenesis in the hippocampal dentate gyrus (DG)of adult rats, 5-bromo-2-deoxyuridine (BrdU), a thymidine analog was applied to mark cell proliferation. Neuroepthelial stem cell protein (nestin) expression was used to identify neural stem/precursor cells. The BrdU- and nestin-positive cells were examined by immunohistochemical technique. The ability of learning was evaluated by Y-maze test to explore the functional role of the newborn cells in the DG after forced running. It was found that the number of BrdU- and nestin-positive cells in the DG in running groups was significantly increased compared to that in the control group (P＜0.05). The effect of forced running on neurogenesis was intensitydependent. In addition, an improvement of learning ability in Y-maze test was observed after forced running. These findings suggest that forced running in motor-driven wheel could enhance neurogenesis in the hippocampal DG of adult rats and improve learning ability.