电针夹脊穴的抗炎镇痛和免疫调节作用
전침협척혈적항염진통화면역조절작용
Effect of electropumcture at Jiaji acupoint on anti-inflammation, analgesia and immunoloregulation
  • 万方数据
    中国临床康复 중국림상강복
    CHINESE JOURNAL OF CLINICAL REHABILITATION
    2006年 19期 171-173 ,共3页

    吕玉玲%赵鲁明%陈洪沛%房繄恭%吴富东

    려옥령%조로명%진홍패%방예공%오부동

    关节炎%佐剂性/针灸疗法%电针%疾病模型%动物%镇痛%消炎%T淋巴细胞亚群 關節炎%佐劑性/針灸療法%電針%疾病模型%動物%鎮痛%消炎%T淋巴細胞亞群
    관절염%좌제성/침구요법%전침%질병모형%동물%진통%소염%T림파세포아군
    背景:临床观察证实,针灸具有确切的抗炎和免疫调节的作用,这是针灸防治各类免疫失调和急慢性炎症的基础.目的:观察电针夹脊穴对佐剂性关节炎大鼠的抗炎镇痛作用及对其T细胞亚群的影响.设计:完全随机分组设计,对照实验.单位:山东中医药大学针灸基础实验室.材料:选用纯种Wistar大鼠30只.适应性饲养5 d后,随机将大鼠分为3组:正常对照组,模型组,电针夹脊穴组,每组10只.方法:①实验于2005-01/2005-05在山东中医药大学针灸基础实验室完成.模型组和电针夹脊穴组用弗氏完全佐剂造成佐剂性关节炎模型.于造模当天,电针夹脊穴组针刺双侧第3,5腰椎夹脊穴,用28号0.5寸(约1.65 cm)不锈钢毫针在大鼠第3,5腰椎棘突下旁开约0.3 cm直刺进针,接G6805-2A型多功能电针治疗仪给予疏密波(频率约4 Hz,密波频率约60 Hz),强度约为1 mA,30 min/次,1次/d,连续治疗7 d.正常对照组及模型组以相同的方式捆绑固定(用粗布绳将大鼠捆绑固定于大鼠固定器)7 d.②于造模前,造模后1,7 d进行痛阈测定:采用热痛刺激仪的强光照射大鼠足垫部,将大鼠的缩爪潜伏期作为痛阈值.③造模前,造模后1,7 d采用鼠足容积测定器测定大鼠右后爪足容积(容积排水法),计算肿胀率(%)=(造模后鼠足容积-造模前鼠足容积)/造模前鼠足容积×100%.④于造模后8 d,采用FACSCalibur流式细胞仪检测血清CD4+,CD8+细胞表达率并计算CD4+/CD8+.③多个样本均数比较采用单因素方差分析和t检验.主要观察指标:电针夹脊穴对佐剂性关节炎大鼠痛阈、足肿胀率及血清T细胞亚群的影响.结果:大鼠30只均进入结果分析.①造模后1 d,模型组、电针夹脊穴组大鼠右后足足容积明显高于正常对照组和造模前(P＜0.01);肿胀率明显高于正常对照组(P＜0.01).造模后7 d,模型组右后足容积及足爪肿胀率明显高于正常对照组(P＜0.01),足容积明显高于造模前(P＜0.01);电针夹脊穴组右后足足容积和肿胀率明显低于模型组(P＜0.05).②正常大鼠造模前双后足痛阈无明显差异,造模后1 d模型组和电针夹脊穴组大鼠致炎侧足痛阈较致炎前显著降低(P＜0.01),明显低于正常对照组(P＜0.01);造模后7 d模型组大鼠致炎侧足痛阈仍明显底于致炎前(P＜0.05);电针夹脊穴组痛阈明显高于模型组(P＜0.05).③模型组大鼠CD4+T淋巴细胞及CD8+T淋巴细胞百分率明显低于正常对照组显著降低(P＜0.01),CD4+/CD8+的比值明显高于正常对照组(P＜0.05).电针夹脊穴组CD4+细胞百分率高于模型组,但差异不明显,CD8+细胞百分率明显高于模型组(P＜0.01),CD4+与CD8+比值明显低于模型组(P＜0.05).结论:电针夹脊穴有明显的抗炎镇痛作用,并能调整佐剂性关节炎大鼠的细胞免疫.
    배경:림상관찰증실,침구구유학절적항염화면역조절적작용,저시침구방치각류면역실조화급만성염증적기출.목적:관찰전침협척혈대좌제성관절염대서적항염진통작용급대기T세포아군적영향.설계:완전수궤분조설계,대조실험.단위:산동중의약대학침구기출실험실.재료:선용순충Wistar대서30지.괄응성사양5 d후,수궤장대서분위3조:정상대조조,모형조,전침협척혈조,매조10지.방법:①실험우2005-01/2005-05재산동중의약대학침구기출실험실완성.모형조화전침협척혈조용불씨완전좌제조성좌제성관절염모형.우조모당천,전침협척혈조침자쌍측제3,5요추협척혈,용28호0.5촌(약1.65 cm)불수강호침재대서제3,5요추극돌하방개약0.3 cm직자진침,접G6805-2A형다공능전침치료의급여소밀파(빈솔약4 Hz,밀파빈솔약60 Hz),강도약위1 mA,30 min/차,1차/d,련속치료7 d.정상대조조급모형조이상동적방식곤방고정(용조포승장대서곤방고정우대서고정기)7 d.②우조모전,조모후1,7 d진행통역측정:채용열통자격의적강광조사대서족점부,장대서적축조잠복기작위통역치.③조모전,조모후1,7 d채용서족용적측정기측정대서우후조족용적(용적배수법),계산종창솔(%)=(조모후서족용적-조모전서족용적)/조모전서족용적×100%.④우조모후8 d,채용FACSCalibur류식세포의검측혈청CD4+,CD8+세포표체솔병계산CD4+/CD8+.③다개양본균수비교채용단인소방차분석화t검험.주요관찰지표:전침협척혈대좌제성관절염대서통역、족종창솔급혈청T세포아군적영향.결과:대서30지균진입결과분석.①조모후1 d,모형조、전침협척혈조대서우후족족용적명현고우정상대조조화조모전(P＜0.01);종창솔명현고우정상대조조(P＜0.01).조모후7 d,모형조우후족용적급족조종창솔명현고우정상대조조(P＜0.01),족용적명현고우조모전(P＜0.01);전침협척혈조우후족족용적화종창솔명현저우모형조(P＜0.05).②정상대서조모전쌍후족통역무명현차이,조모후1 d모형조화전침협척혈조대서치염측족통역교치염전현저강저(P＜0.01),명현저우정상대조조(P＜0.01);조모후7 d모형조대서치염측족통역잉명현저우치염전(P＜0.05);전침협척혈조통역명현고우모형조(P＜0.05).③모형조대서CD4+T림파세포급CD8+T림파세포백분솔명현저우정상대조조현저강저(P＜0.01),CD4+/CD8+적비치명현고우정상대조조(P＜0.05).전침협척혈조CD4+세포백분솔고우모형조,단차이불명현,CD8+세포백분솔명현고우모형조(P＜0.01),CD4+여CD8+비치명현저우모형조(P＜0.05).결론:전침협척혈유명현적항염진통작용,병능조정좌제성관절염대서적세포면역.
    BACKGROUND: Clinical observation has been proved that acupuncture has effect on antiinflammation and immunoloregulation, which is the basis for preventing and curing immune disturbance and active chronic inflammation. OBJECTIVE: To observe the effect of electropumcture on antiinflammation, analgesia and subgroup of T cells of adjuvant-arthrosis rats at Jiaji acupoint.DESIGN: Completely randomized grouping and controlled study.SETTING: Basic Laboratory of Acupuncture and Moxibustion, Shandong University of Traditional Chinese Medicine. MATERIALS: A total of 30 Wistar rats were selected in this study. Five days after feeding, all rats were randomly divided into 3 groups: normal control group, model group and electropumcture group with 10 in each group. METHODS: ① The experiment was completed at Basic Laboratory of Acupuncture and Moxibustion of Shandong University of Traditional Chinese Medicine from January to May 2005. Freund's complete adjuvant (FCA) was used to establish adjuvant-arthrosis models in model group and electropumcture group. On the modeling day, the 3rd and the 5th Jiaji acupoints of bilateral lumbar vertebrae of rats in electropumcture group were acupunctured with 28-sized stainless-steel milli-needle of 0.5 inch.The needle was stimulated 0.3 cm from the 3rd and the 5th spinous process of lumbar vertebra which was counected to G6805-2A multiple functional electropumcture meter for sparse-tight waves (frequency of sparse wave: 4 Hz, frequency of tight wave: 60 Hz, intensity: t mA) 30 minutes each time,once a day for 7 days. Rats in normal group and model group were fixed with the same way for 7 days. (Rats were fixed with cloth-rope at fixing apparatus.) ② Pain threshold was measured before modelling, 1 and 7 days after modelling. Foot pad was exposured with strong light by hot-pain stimulation meter, and paw withdrawal latency was regarded as pain threshold. ③ Right hindfoot bulk of rats was assayed with foot bulk determinator (bulk draining method) before modelling, 1 and 7 days after modelling to calculate swelling rate (％) [(foot bulk after modelling-foot bulk before modelling)/ foot bulk before modelling × 100％ ]. ④ Expressional rates of serum CD4+ and CD8+ were assayed with FACSCalibur flow cytometer and the ratio between CD4+ and CD8+ was calculated 8 days after modelling. ⑤ Average value of multiple samples were compared with single-factor analysis of variance and t test.MAIN OUTCOME MEASURES: Effect of electropumcture at Jiaji acupoint on pain threshold, swelling rate and subgroup of serum T cells of adjuvant-arthrosis rats.RESULTS: A total of 30 rats were involved in the final analysis. ① One day after modeling, bulk of right hindfoot in model group and electropumcture group was bigger than that in normal control group and that before modeling (P ＜ 0.01), and swelling rate was higher than that in normal control group (P ＜ 0.01); 7 days after modeling, bulk and swelling of right hindfoot in model group were higher than those in normal control group (P ＜ 0.01), and bulk was bigger than that before modeling (P ＜ 0.01);bulk and swelling of right hindfoot in electropumcture group were lower than those in model group (P ＜ 0.05). ② Pain threshold of both rear feet of normal rats were not changed after modelling. One day after modelling,pain threshold at inflammatory side was decreased in model group and electropumcture group (P ＜ 0.01) and it was lower than that in normal control group (P ＜ 0.01); 7 days after modeling, pain threshold at inflammatory side was still lower than that before inflammation (P ＜ 0.05),and it in electropumcture group was higher than that in model group (P＜ 0.05). ③ Percentages of CD4+ T lymphocyte and CD8+ T lymphocyte in model group were lower than those in normal control group (P ＜ 0.01), and ratio of CD4+/CD8+ was also higher than that in normal control group (P＜ 0.05). Percentage of CD4+ in electropumcture group was higher than that in model group, but there was no significant difference. Percentage of CD8+ in electropumcture group was higher than that in model group (P ＜ 0.01),but ratio of CD4+/CD8+ was lower than that in model group (P ＜ 0.05).CONCLUSION: Electropumcture at Jiaji acupoint has obvious effect on anti-inflammation and analgesia, and can also regulate cellular immunity of adjuvant-arthrosis rats.
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