中国人兽共患病学报
中國人獸共患病學報
중국인수공환병학보
CHINESE JOURNAL OF ZOONOSES
2007年
7期
696-698
,共3页
曹得萍%赤尾信明%太田伸生
曹得萍%赤尾信明%太田伸生
조득평%적미신명%태전신생
犬蛔虫%plate-ELISA%IgG%IgE%Western-blot
犬蛔蟲%plate-ELISA%IgG%IgE%Western-blot
견회충%plate-ELISA%IgG%IgE%Western-blot
Toxocara canis%plate-ELISA%IgG%IgE%western-blot
目的 研究犬蛔虫眼病病人血清中特异性IgG和IgE的抗体的水平.方法 应用ELISA和Western-blotting.从东京医科齿科大学国际环境寄生虫学分野实验室储存的脉络膜炎的眼病病人血清中随意抽取105份血清,检测犬蛔虫抗体.结果 通过ELISA法,105份血清中有82份IgG和IgE均为阴性(78.1%),12例血清仅IgG阳性(11.4%),3例血清仅IgE阳性(2.9%),8例血清IgG和IgE均为阳性.所有的IgG和IgE阳性的血清,进一步进行Western-blot.IgG的反应带分布在幼虫分泌排泄抗原分子重量(97.2-14.3kDa)的整个范围内,IgE的反应带分布在幼虫分泌排泄抗原分子重量(97.2-14.3kDa)的相对狭窄的范围内(29-45kDa).结论 在这个研究中,我们清楚的说明了一些脉络膜炎病例血清中IgE抗体比IgG抗体具有特异性,因此IgE在眼犬蛔虫病的诊断中具有特异性,IgE在眼犬蛔虫病诊断中的价值还要进行进一步的研究.
目的 研究犬蛔蟲眼病病人血清中特異性IgG和IgE的抗體的水平.方法 應用ELISA和Western-blotting.從東京醫科齒科大學國際環境寄生蟲學分野實驗室儲存的脈絡膜炎的眼病病人血清中隨意抽取105份血清,檢測犬蛔蟲抗體.結果 通過ELISA法,105份血清中有82份IgG和IgE均為陰性(78.1%),12例血清僅IgG暘性(11.4%),3例血清僅IgE暘性(2.9%),8例血清IgG和IgE均為暘性.所有的IgG和IgE暘性的血清,進一步進行Western-blot.IgG的反應帶分佈在幼蟲分泌排洩抗原分子重量(97.2-14.3kDa)的整箇範圍內,IgE的反應帶分佈在幼蟲分泌排洩抗原分子重量(97.2-14.3kDa)的相對狹窄的範圍內(29-45kDa).結論 在這箇研究中,我們清楚的說明瞭一些脈絡膜炎病例血清中IgE抗體比IgG抗體具有特異性,因此IgE在眼犬蛔蟲病的診斷中具有特異性,IgE在眼犬蛔蟲病診斷中的價值還要進行進一步的研究.
목적 연구견회충안병병인혈청중특이성IgG화IgE적항체적수평.방법 응용ELISA화Western-blotting.종동경의과치과대학국제배경기생충학분야실험실저존적맥락막염적안병병인혈청중수의추취105빈혈청,검측견회충항체.결과 통과ELISA법,105빈혈청중유82빈IgG화IgE균위음성(78.1%),12례혈청부IgG양성(11.4%),3례혈청부IgE양성(2.9%),8례혈청IgG화IgE균위양성.소유적IgG화IgE양성적혈청,진일보진행Western-blot.IgG적반응대분포재유충분비배설항원분자중량(97.2-14.3kDa)적정개범위내,IgE적반응대분포재유충분비배설항원분자중량(97.2-14.3kDa)적상대협착적범위내(29-45kDa).결론 재저개연구중,아문청초적설명료일사맥락막염병례혈청중IgE항체비IgG항체구유특이성,인차IgE재안견회충병적진단중구유특이성,IgE재안견회충병진단중적개치환요진행진일보적연구.
To investigate the prevalence of specific anti-Toxocara IgG and IgE antibodies in ocular toxocariasis by means of ELISA and Western blotting, serum samples of 105 cases with uveitis of unidentified etiology were randomly selected from our stocked sera, which were referred to the Section of of Environmental Parasitology of Tokyo Medical and Dental University for detection of the anti-Toxocara antibody. By using ELISA, 82 of them (78.1%) were negative for both IgG and IgE antibodies, 12 (11.4%) were positive only for IgG, three (2.9%) were positive only for IgE, and eight (7.6%) were positive for IgG and IgE. Among the positive samples, as demonstrated by western bloting the IgG reacting bands were found to distribute in the whole range of molecular weights (97.2-14.3kDa)of excretory-secretory products of T. canis larvae. On the other hand,IgE antigenic molecules were concentrated on a relatively narrow range from 45kDa to 29kDa. In this study, we clearly demonstrated that some of the patients with uveitis showed specific anti-Toxocara IgE antibody but not IgG antibody in serum sample, suggesting that the demonstration of specific IgE antibody should be considered for the precise diagnosis of ocular toxocariasis. Further studies are needed to clarify the diagnosis significance of specific IgE antibody in ocular toxocariasis.
