国际检验医学杂志
國際檢驗醫學雜誌
국제검험의학잡지
INTERNATIONAL JOURNAL OF LABORATORY MEDICINE
2009年
7期
651-652,654
,共3页
红斑狼疮%系统性%抗体,抗中性白细胞胞质%临床实验室技术
紅斑狼瘡%繫統性%抗體,抗中性白細胞胞質%臨床實驗室技術
홍반랑창%계통성%항체,항중성백세포포질%림상실험실기술
Lupus Erythematosus%Systemic%Antineutrophil Cytoplasmic Antibody%Clinical Laboratory Techniques
目的 探讨抗中性粒细胞胞浆抗体(ANCA)在系统性红斑狼疮(SLE)患者中检测的临床意义.方法 通过间接免疫荧光法和免疫印迹法分别检测了25例健康对照组和106例SLE患者血清中的ANCA、抗核抗体(ANA)、抗双链DNA抗体、抗-SmDI抗体.结果 对照组测定均为阴性.SLE患者中ANCA阳性率为47.2%(50/106),且均为环细胞核着色的核周型(pANCA);在ANCA阳性组及ANCA阴性组中:ANA及抗-SmDI抗体阳性率分别为100%(50/50)、91.1%(51/56):82%(41/50)、67.9%(38/56),P均＞0.05;抗ds-DNA抗体阳性率分别为94%(47/50)、50%(28/56),P＜0.01.在活动性SLE(52例)和非活动性SLE(54例)中ANCA阳性率分别为84.6%(44/52);11.1%(6/54),P＜0.01.结论 ANCA在SLE患者中检测与ANA及抗-SmDI抗体没有相关性,与抗dsDNA抗体正相关,活动期SLE患者中ANCA阳性率明显高于非活动期的患者.
目的 探討抗中性粒細胞胞漿抗體(ANCA)在繫統性紅斑狼瘡(SLE)患者中檢測的臨床意義.方法 通過間接免疫熒光法和免疫印跡法分彆檢測瞭25例健康對照組和106例SLE患者血清中的ANCA、抗覈抗體(ANA)、抗雙鏈DNA抗體、抗-SmDI抗體.結果 對照組測定均為陰性.SLE患者中ANCA暘性率為47.2%(50/106),且均為環細胞覈著色的覈週型(pANCA);在ANCA暘性組及ANCA陰性組中:ANA及抗-SmDI抗體暘性率分彆為100%(50/50)、91.1%(51/56):82%(41/50)、67.9%(38/56),P均＞0.05;抗ds-DNA抗體暘性率分彆為94%(47/50)、50%(28/56),P＜0.01.在活動性SLE(52例)和非活動性SLE(54例)中ANCA暘性率分彆為84.6%(44/52);11.1%(6/54),P＜0.01.結論 ANCA在SLE患者中檢測與ANA及抗-SmDI抗體沒有相關性,與抗dsDNA抗體正相關,活動期SLE患者中ANCA暘性率明顯高于非活動期的患者.
목적 탐토항중성립세포포장항체(ANCA)재계통성홍반랑창(SLE)환자중검측적림상의의.방법 통과간접면역형광법화면역인적법분별검측료25례건강대조조화106례SLE환자혈청중적ANCA、항핵항체(ANA)、항쌍련DNA항체、항-SmDI항체.결과 대조조측정균위음성.SLE환자중ANCA양성솔위47.2%(50/106),차균위배세포핵착색적핵주형(pANCA);재ANCA양성조급ANCA음성조중:ANA급항-SmDI항체양성솔분별위100%(50/50)、91.1%(51/56):82%(41/50)、67.9%(38/56),P균＞0.05;항ds-DNA항체양성솔분별위94%(47/50)、50%(28/56),P＜0.01.재활동성SLE(52례)화비활동성SLE(54례)중ANCA양성솔분별위84.6%(44/52);11.1%(6/54),P＜0.01.결론 ANCA재SLE환자중검측여ANA급항-SmDI항체몰유상관성,여항dsDNA항체정상관,활동기SLE환자중ANCA양성솔명현고우비활동기적환자.
Objective To study the diagnostic value of anti-neutrophil cytoplasmic antibody (ANCA) detction in patients with systemic lupus erythematosus (SLE). Methods Indirect immunofluorescence (IIF) and Western blotting were applied to detecting the expression of sera ANCA, antinuclear antibody (ANA), anti-double-stranded DNA antibody, anti-SmithD1 antibody in 25 samples of control group and 106 samples of patients with SLE. Results ALL samples of control group showed negative; 47.2% (50/106) sera showed ANCA positive in patients with SLE,and all of them were peripheral nuclear pattern (pANCA). In ANCA positive group, the positive rate of ANA, anti-SmithD1 antibody and anti-ds-DNA antibody was respectively 100% (50/50), 82% (41/50) and 94% (47/50); in ANCA negative group, the positive rate was respectively 91.1% (51/56), 67.9% (38/56)and 50% (28/56). There were no statistical differences in positive rates of ANA and anti-SmithD1 antibody between ANCA positive and negative groups (both P＞0. 05), but there was in positive rates of anti-ds-DNA antibody (P＜0.01). ANCA positive rate was respectively 84.6% (44/52) and 11.1%(6/54) in active SLE group (n=52) and inactive SLE group (n=54) (P＜0.01). Conclusion The determined ANCA in patients with SLE is not correlative with ANA and anti-SmithD1 antibody, but positively relates to anti-double-stranded DNA antibody. The positive rate of ANCA in patients with active lupus is significantly higher than that in non-active lupus.
