CAJ | 학술논문

目的研究水蛭多肽对大鼠局灶性脑缺血再灌注损伤的保护作用,并探讨其作用机制.方法线栓法制作大鼠大脑中动脉缺血再灌注模型.缺血再灌注损伤后24 h,进行神经功能评分,测定脑含水量、缺血面积、脑、组织匀浆超氧化物歧化酶活性和丙二醛含量.结果水蛭多肽能显著降低组脑组织含水量,缩小脑梗死面积,提高超氧化物歧化酶活性,降低丙二醛含量.结论水蛭多肽对于大鼠脑缺血再灌注损伤具有保护作用,其作用机制可能与抑制脂质过氧化、提高抗氧化酶活性有关.
목적 연구수질다태대대서국조성뇌결혈재관주손상적보호작용,병탐토기작용궤제.방법 선전법제작대서대뇌중동맥결혈재관주모형.결혈재관주손상후24 h,진행신경공능평분,측정뇌함수량、결혈면적、뇌、조직균장초양화물기화매활성화병이철함량.결과 수질다태능현저강저조뇌조직함수량,축소뇌경사면적,제고초양화물기화매활성,강저병이철함량.결론 수질다태대우대서뇌결혈재관주손상구유보호작용,기작용궤제가능여억제지질과양화、제고항양화매활성유관.
Purpose To observe the protective effects of low molecular hirudo peptides on focal cerebral ischemia reperfusion injury in rats and to explore its possible mechanism. Methods Middle cerebral artery occlusion (MCAO) was used to prepare focal cerebral ischemia and the neurological scores and infarction area of brain slices, and water content of brains were assessed. Superoxide dismustase (SOD) activity and malondi-aldehyde ( MDA) content in homogenate of ischemic brain tissue were determined by spectrophotometric assay. Results Hirudo peptides could reduce the percentage of infarction area and the water content in the cerebral hemisphere, increase SOD activity and decrease MDA content in ischemic brain tissue. Conclusion Low molecular hirudo peptides have protective effects on focal cerebral ischemia injury, and its mechanism may be related to the antioxidant action.