CAJ | 학술논문

以团花树(Anthocephalus chinensis)枝条形成层的cDNA为模板,参照α-Expansm基因(EXPA)序列设计兼并引物,进行RT-PCR.以得到的扩增产物为基础,采用RACE技术得到扩展蛋白基因全长cDNA,命名为AcEXPl.AcEXPl全长979 bp(GenBank注册号为FJ417847),开放阅读框为777 bp,编码258个氨基酸,并具有Expansin特有的保守序列和结构域.经过比对分析,该基因编码的氡基酸序列与毛白杨、欧洲山杨×美洲山杨、樱桃和矮牵牛的α-Expansin基因编码的氨基酸同源性分别为85%、85%、84%和83%.这为研究EXP基因与团花树木质部生长速度和材质的关系提供了基础.
이단화수(Anthocephalus chinensis)지조형성층적cDNA위모판,삼조α-Expansm기인(EXPA)서렬설계겸병인물,진행RT-PCR.이득도적확증산물위기출,채용RACE기술득도확전단백기인전장cDNA,명명위AcEXPl.AcEXPl전장979 bp(GenBank주책호위FJ417847),개방열독광위777 bp,편마258개안기산,병구유Expansin특유적보수서렬화결구역.경과비대분석,해기인편마적동기산서렬여모백양、구주산양×미주산양、앵도화왜견우적α-Expansin기인편마적안기산동원성분별위85%、85%、84%화83%.저위연구EXP기인여단화수목질부생장속도화재질적관계제공료기출.
Using the cDNA from the branch cambium of Anthocephalus chinensis as the template, the degenerate primers by reference to the α-Expansin gene (EXPA) sequences in other plants were constructed for RT-PCR. Taking the production of the amplification as the starting information, The full-length cDNA of expansin gene was obtained by RACE, named as AcEXPl. It was 979 bp in length (GenBank accession No. FJ417847) with 777 bp ORF, encoding 258 amino acid, which had the typical conserved sequence and domain of Expansin. There were 85%, 85%, 84%, 83% homobgy with Popuha tomentosa, Populus tremula P. tremubides, Prunus cerasus, Petunia hybrida, respectively. It provided the foundation for studying the relationships between EXP gene and growth rate, wood quality of the xylem of Anthocephalus chinensis.