CAJ | 학술논문

整合子是介导细胞多重耐药的重要机制之一,鉴定了2008年某医院重症监护病房分离的23株多重耐药铜绿假单胞菌1型整合子,并应用脉冲场电泳分析其同源性.1型整合子的阳性率达成60.9％.3种1型整合子基因盒被鉴定,其中整合子blaOXA-10-acc6-Ⅱ-cmlA8为首次发现报道.基因盒主要编码氨基糖苷类耐药基因,包括aacA4,aadA2,aadB,aac6-Ⅱ.脉冲电泳结果表明,23株多重耐药铜绿假单胞菌分为5个基因型,A型(n=5)、B型(n=6)、C型(n=4)、D型(n=2)和E型(n=2).研究表明编码1型整合子是多重耐药铜绿单胞菌较为普遍的特征,且1型整合子与多重耐药表型存相关.研究结果同时也表明防止多重耐药铜绿单胞菌交叉感染仍然是ICU的一项挑战性工作.
정합자시개도세포다중내약적중요궤제지일,감정료2008년모의원중증감호병방분리적23주다중내약동록가단포균1형정합자,병응용맥충장전영분석기동원성.1형정합자적양성솔체성60.9％.3충1형정합자기인합피감정,기중정합자blaOXA-10-acc6-Ⅱ-cmlA8위수차발현보도.기인합주요편마안기당감류내약기인,포괄aacA4,aadA2,aadB,aac6-Ⅱ.맥충전영결과표명,23주다중내약동록가단포균분위5개기인형,A형(n=5)、B형(n=6)、C형(n=4)、D형(n=2)화E형(n=2).연구표명편마1형정합자시다중내약동록단포균교위보편적특정,차1형정합자여다중내약표형존상관.연구결과동시야표명방지다중내약동록단포균교차감염잉연시ICU적일항도전성공작.
To characterize class 1 integrons in multi-drug resistant (MDR) P.aeruginosa in an intensive care unit of a teaching hospital.23 strain of MDR P.aeruginosa recovered from patients in intensive care unit (ICU) were collected during 2008.The gene cassettes and antibiotic susceptibility of the isolates were characterized.All isolates were also subjected to pulsed-field gel electrophoresis (PFGE).Class 1 integrons were detected in 14 isolates (60.9％ ).Three types of gene cassettes were identified among these strains,including one that has not been reported previously.The vast majority of the cassettes encoded aminoglycoside resistance gene,including aacA4,aadA2,aadB,aac6-Ⅱ.Most of isolates were classified into five PFGE patterns,type A ( n =6 ) ; type B ( n =6 ) ; type C ( n =4 ) ; type D ( n =2 )and type E (n =2).The present study illustrates that integrons appear to be a common feature among MDR P.aeruginosa in Guangzhou of China,and associated with a high prevalence of antibiotic resistance.Our data also suggested that cross-infection of MDR P.aeruginosa was a challenging work in ICU.