CAJ | 학술논문

建立高脂诱导载脂蛋白E基因敲除(ApoE-/-)小鼠胰岛素抵抗模型,探讨其对胆固醇代谢相关基因表达的影响.结果显示高脂喂养组ApoE-/-小鼠空腹血糖、游离脂防酸、总胆固醇、甘油三酯、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)和空腹血浆胰岛素水平明显高于对照组(均P＜0.01);肝组织中INSIG2和SCAP mRNA明显升高(P＜0.05或P＜0.01),肝INSIG2蛋白水平也明显增高(P＜0.05);而SREBP1 mRNA表达明显降低(P＜0.05).这些改变可能在该小鼠模型胆固醇代谢紊乱和胰岛素抵抗的发生中具有一定的作用.
건립고지유도재지단백E기인고제(ApoE-/-)소서이도소저항모형,탐토기대담고순대사상관기인표체적영향.결과 현시고지위양조ApoE-/-소서공복혈당、유리지방산、총담고순、감유삼지、저밀도지단백담고순(LDL-C)、고밀도지단백담고순(HDL-C)화공복혈장이도소수평명현고우대조조(균P＜0.01);간조직중INSIG2화SCAP mRNA명현승고(P＜0.05혹P＜0.01),간INSIG2단백수평야명현증고(P＜0.05);이SREBP1 mRNA표체명현강저(P＜0.05).저사개변가능재해소서모형담고순대사문란화이도소저항적발생중구유일정적작용.
To investigate the effect on gene expression related cholesterol metabolism in ApoE-/- mice with high-fat-induced insulin resistance(IR). In high-fat fed mice, FBG, FFA, TC, TG, LDL-C, HDL-C, and fasting plasma insulin were significantly higher than those of controls(P＜0.01). The INSIG2 and SCAP mRNA expressions in liver were significantly increased in high-fat fed mice compared with controls(P＜0. 05 or P＜0.01), and INSIG2 protein levels also increased(P＜0. 05). But SREBP1 mRNA expression in liver of high-fat fed mice was significantly reduced(P＜0. 05). These changes might contribute to IR and disorder of cholesterol metabolism in high-fat fed ApoE-/- mice.