CAJ | 학술논문

目的探讨姜黄素对自发性高血压(SH)大鼠脑缺血再灌注时海马神经元凋亡及c-Jun 氨基末端激酶3(JNK3)和突触后密度蛋白95(PSD95)表达的影响.方法与雄性WKY同源的SH大鼠135只和雄性WKY大鼠90只,清洁级,体重275～325 g,采用随机数字表法,将WKY大鼠随机分为2组(n=45):假手术组(W-S组)及脑缺血再灌注组(W-I/R组),将SH大鼠随机分为3组(n=45):假手术组(S-S组)和脑缺血再灌注组(S-I/R组)及姜黄素组(S-C组).采用四血管阻断法制备全脑缺血再灌注模型.W-S组和S-S组仅分离双侧颈总动脉,W-I/R组和S-I/R组于再灌注30 min时腹腔注射玉米油10 ml/kg,S-C组于再灌注30 min时腹腔注射姜黄素100 mg/kg.于再灌注2 h,6 h、1 d、3 d和7d时进行海马凋亡神经元计数,并测定海马JNK3和PSD95蛋白的表达水平.结果与W-S组比较,S-S组海马凋亡神经元计数增加(P<0.05),JNK3蛋白表达差异无统计学意义(P>0.05);与S-S组比较,S-I/R组海马凋亡神经元计数增加,JNK3蛋白表达上调(P<0.05);与S-I/R组比较,S-C组海马凋亡神经元计数减少,JNK3蛋白表达下调(P<0.05).各组海马PSD95蛋白表达比较差异无统计学意义(P>0.05).结论姜黄素可抑制SH大鼠脑缺血再灌注时神经元凋亡,其机制与下调海马JNK3蛋白表达有关,姜黄素下调海马JNK3蛋白表达可能与PSD95途径无关.
목적 탐토강황소대자발성고혈압(SH)대서뇌결혈재관주시해마신경원조망급c-Jun 안기말단격매3(JNK3)화돌촉후밀도단백95(PSD95)표체적영향.방법 여웅성WKY동원적SH대서135지화웅성WKY대서90지,청길급,체중275～325 g,채용수궤수자표법,장WKY대서수궤분위2조(n=45):가수술조(W-S조)급뇌결혈재관주조(W-I/R조),장SH대서수궤분위3조(n=45):가수술조(S-S조)화뇌결혈재관주조(S-I/R조)급강황소조(S-C조).채용사혈관조단법제비전뇌결혈재관주모형.W-S조화S-S조부분리쌍측경총동맥,W-I/R조화S-I/R조우재관주30 min시복강주사옥미유10 ml/kg,S-C조우재관주30 min시복강주사강황소100 mg/kg.우재관주2 h,6 h、1 d、3 d화7d시진행해마조망신경원계수,병측정해마JNK3화PSD95단백적표체수평.결과 여W-S조비교,S-S조해마조망신경원계수증가(P<0.05),JNK3단백표체차이무통계학의의(P>0.05);여S-S조비교,S-I/R조해마조망신경원계수증가,JNK3단백표체상조(P<0.05);여S-I/R조비교,S-C조해마조망신경원계수감소,JNK3단백표체하조(P<0.05).각조해마PSD95단백표체비교차이무통계학의의(P>0.05).결론 강황소가억제SH대서뇌결혈재관주시신경원조망,기궤제여하조해마JNK3단백표체유관,강황소하조해마JNK3단백표체가능여PSD95도경무관.
Objective To investigate the effect of curcumin on apoptosis in hippocampal neurons and the expression of c-Jun N-terminal kinase 3 (JNK3) and postsynaptic density protein 95 (PSD95) in hippocampus during cerebral ischemia-reperfusion (I/R) in rats with spontaneous hypertension (SH) .Methods One hundred and thirty-five male rats (homologous with WKY) with SH and 90 male normotensive WKY rats, weighing 275-325 g,were used in this study. The WKY rats were randomized into 2 groups ( n = 45 each) : sham operation group (WS group) and cerebral I/R group (W-I/R group) . The rats with SH were randomly divided into 3 groups ( n = 45each) : sham operation group (S-S group), cerebral I/R group (S-I/R group) and curcumin group (S-C group) .Global cerebral ischemia was produced by 4 vessel-occlusion method. The bilateral common carotid arteries were only exposed but not ligated in W-S and S-S groups. Intraperitoneal corn oil 10 ml/kg was injected at 30 min of reperfusion in W-I/R and S-I/R groups. Intraperitoneal curcumin 100 mg/kg was injected at 30 min of reperfusion in S-C group. Three animals in each group were sacrificed at 2 h, 6 h, 1 d, 3 d and 7 d of reperfusion and their brains were harvested for determination of apoptosis in hippocampal neurons and the expression of JNK3 and PSD95in hippocampus. Results The number of apoptotic neurons was significantly increased in S-S group compared with W-S group ( P < 0.05) . The number of apoptotic neurons was significantly increased and the expression of JNK3was up-regulated in S-I/R group compared with S-S group ( P < 0.05) . The number of apoptotic neurons was significantly decreased and the expression of JNK3 was down-regulated in S-C group compared with S-I/R group (P <0.05) . There was no significant difference in the expression of PSD95 among all the groups ( P > 0.05) . Conclusion Curcumin can inhibit apoptosis in hippocampal neurons and the mechanism is related to down-regulation of the expression of JNK3 in hippocampus. The mechanism by which curcumin down-regulates the expression of JNK3in hippocampus may not be related to PSD95 pathway.