国际生物制品学杂志
國際生物製品學雜誌
국제생물제품학잡지
INTERNATIONAL JOURNAL OF BIOLOGICALS
2011年
1期
9-12
,共4页
杨宇宸%崔泽林%郭晓奎%刘畅
楊宇宸%崔澤林%郭曉奎%劉暢
양우신%최택림%곽효규%류창
耐药性%抗生素类%菌群%鱼疾病
耐藥性%抗生素類%菌群%魚疾病
내약성%항생소류%균군%어질병
Drug resistance%Antibiotics%Intestinal flora%Fish diseases
目的 调查分析目前国内常见养殖淡水鱼类肠道菌群对不同抗生素的耐药性现状.方法 选取两种常见养殖淡水鱼类的粪便标本,分别在添加了7种抗生素[青霉素G、头孢呋辛钠、庆大霉素、环丙沙星、四环素、红霉素和复方新诺明(SMZ)]的琼脂平板上进行培养,通过PCR法分析所获得的耐药菌株的16S rRNA基因序列并对其种属进行鉴定.采用x2检验作两种鱼之间的比较.结果 筛选后共得到2956株耐药菌,对青霉素G、头孢呋辛钠、四环素、SMZ、庆大霉素、红霉素和环丙沙星的平均耐药率分别为8.89%、1.57%、0.28%、0.06%、0.08%、0.07%和0.11%.用单纯随机法挑选71株耐药菌,培养鉴定后其主要组成为:气单胞菌31株(43.66%)、不动杆菌8株(11.27%)、假单胞菌6株(8.45%)、金黄杆菌6株(8.45%).两种鱼肠道菌群对青霉素G、头孢呋辛钠、SMZ、红霉素和四环素的耐药水平差异有统计学意义(x2值为129.06、212.54、7.76、8.62和35.40,P值均＜0.05).结论 通过获得两种常见养殖淡水鱼类肠道菌群对7种抗生素(青霉素G、头孢呋辛钠、庆大霉素、环丙沙星、四环素、红霉素和SMZ)的耐药率,为今后进一步研究和追踪常见淡水鱼肠道菌群耐药率的变化提供了基础.
目的 調查分析目前國內常見養殖淡水魚類腸道菌群對不同抗生素的耐藥性現狀.方法 選取兩種常見養殖淡水魚類的糞便標本,分彆在添加瞭7種抗生素[青黴素G、頭孢呋辛鈉、慶大黴素、環丙沙星、四環素、紅黴素和複方新諾明(SMZ)]的瓊脂平闆上進行培養,通過PCR法分析所穫得的耐藥菌株的16S rRNA基因序列併對其種屬進行鑒定.採用x2檢驗作兩種魚之間的比較.結果 篩選後共得到2956株耐藥菌,對青黴素G、頭孢呋辛鈉、四環素、SMZ、慶大黴素、紅黴素和環丙沙星的平均耐藥率分彆為8.89%、1.57%、0.28%、0.06%、0.08%、0.07%和0.11%.用單純隨機法挑選71株耐藥菌,培養鑒定後其主要組成為:氣單胞菌31株(43.66%)、不動桿菌8株(11.27%)、假單胞菌6株(8.45%)、金黃桿菌6株(8.45%).兩種魚腸道菌群對青黴素G、頭孢呋辛鈉、SMZ、紅黴素和四環素的耐藥水平差異有統計學意義(x2值為129.06、212.54、7.76、8.62和35.40,P值均＜0.05).結論 通過穫得兩種常見養殖淡水魚類腸道菌群對7種抗生素(青黴素G、頭孢呋辛鈉、慶大黴素、環丙沙星、四環素、紅黴素和SMZ)的耐藥率,為今後進一步研究和追蹤常見淡水魚腸道菌群耐藥率的變化提供瞭基礎.
목적 조사분석목전국내상견양식담수어류장도균군대불동항생소적내약성현상.방법 선취량충상견양식담수어류적분편표본,분별재첨가료7충항생소[청매소G、두포부신납、경대매소、배병사성、사배소、홍매소화복방신낙명(SMZ)]적경지평판상진행배양,통과PCR법분석소획득적내약균주적16S rRNA기인서렬병대기충속진행감정.채용x2검험작량충어지간적비교.결과 사선후공득도2956주내약균,대청매소G、두포부신납、사배소、SMZ、경대매소、홍매소화배병사성적평균내약솔분별위8.89%、1.57%、0.28%、0.06%、0.08%、0.07%화0.11%.용단순수궤법도선71주내약균,배양감정후기주요조성위:기단포균31주(43.66%)、불동간균8주(11.27%)、가단포균6주(8.45%)、금황간균6주(8.45%).량충어장도균군대청매소G、두포부신납、SMZ、홍매소화사배소적내약수평차이유통계학의의(x2치위129.06、212.54、7.76、8.62화35.40,P치균＜0.05).결론 통과획득량충상견양식담수어류장도균군대7충항생소(청매소G、두포부신납、경대매소、배병사성、사배소、홍매소화SMZ)적내약솔,위금후진일보연구화추종상견담수어장도균군내약솔적변화제공료기출.
Objective To study the different antibiotic resistance of the intestinal flora in domestic species of freshwater fish. Methods The fecal specimens were collected from two species of freshwater fish,and were cultured on agar-plate containing 7 kinds of antibiotics ( penicilin G, cefuroxime sodium, gentamicin,ciprofloxacin, tetracycline, erythromycin and SMZ) for the antibiotic susceptibility test. PCR was applied on the results to analyse the 16S rRNA gene sequences of resistant strains and identify their species. x2test was adopt in the comparison between the two fishes. Results 2956 drug-resistant isolates were screened from all of the samples. The rates of resistance to penicilin G, cefuroxime sodium, gentamicin, ciprofloxacin,tetracycline, erythromycin and SMZ were 8.89%, 1.57%, 0.08%, 0. 11%, 0. 28%, 0.07% and 0.06%,respectively. The results of identification showed that the main components of the 71 strains selected by simple random method were 31 strains of Aeromonas (43.66%), 8 strains of Acinetobacter (11.27%), 6 strains of Pseudomonas (8.45%) and 6 strains of Chryseobacterium (8.45%). There were statistically significant differences in the rates of resistance to penicilin G, cefuroxime sodium, SMZ, erythromycin and tetracycline between the two kinds of fish (x2 values were 129.06,212. 54,7. 76,8. 62 and 35.40, P＜0. 05).Conclusions The rates of resistance to penicilin G, cefuroxime sodium, gentamicin, ciprofoxacin,tetracycline, erythromycin and SMZ of the intestinal flora in domestic species of freshwater fish we obtained can provide foundation for us to do some researches and pursue the changes of the antibiotic resistance rates of the intestinal flora in domestic species of freshwater fish in future.
