目的 研究低硒、低碘及其联合对亲代和子代大鼠骨、软骨生长的影响.方法 48只断乳SD健康大鼠,按体质量随机分对照组、低硒组、低碘组和低硒低碘组,每组12只.4组大鼠分别采用人工配制的低硒、低碘、低硒低碘及含硒、碘量正常的饲料喂饲.大鼠饲养8周后(约3月龄)进行组内繁殖;亲代大鼠喂饲至6月龄、子代大鼠喂饲至3月龄时,取亲代和子代大鼠血样,测定血清硒及T3、T4;取大鼠右侧胫骨及左侧膝关节.用游标卡尺测定胫骨长度、中段额状横径、胫骨上端关节软骨横径、纵径;膝关节包埋切片后,光镜下测定胫骨近端生长板厚度、生长板软骨肥大层、增殖层细胞层数.结果 低硒对亲代和子代大鼠血清硒有明显影响(F值分别239.56、232.68,P均<0.05),对于代血清T4水平也有明显影响(F=6.95,P<0.05);低碘对亲代和子代大鼠血清T3、T4均有明显影响(F值分别为14.11、14.05,30.29、34.53,P<0.01),低硒、低碘联合对子代大鼠血清T4水平的影响存在交互作用(F=5.99,P<0.01).亲代和子代大鼠血清硒,低硒组[(30.28±6.34)、(43.95±9.75)μg/L]、低硒低碘组[(30.33±5.18)、(35.40±3.06)μg/L]均明显低于低碘组[(345.83±29.55)、(245.24±9.95)μg/L]、对照组[(358.64±30.50)、(236.50±9.75),P均<0.05].低硒低碘组[(0.55±0.05)、(0.88±0.14)mmol/L]亲代和子代大鼠血清T3均明显低于对照组[(0.75±0.08)、(1.26±0.26)mmol/L,P均<0.05],低碘组[(24.11±2.29)、(42.10±8.92)mmol/L]、低硒低碘组[(20.66±1.93)、(26.55±5.98)mmol/L]亲代和子代大鼠血清T4均明显低于对照组[(36.15±2.74)、(52.79±8.84)mmol/L]和低硒组[(28.12±3.33)、(52.02±11.99)mmol/L,P均<0.05];低硒低碘组子代大鼠血清T4明显低于低碘组(P<0.05).子代大鼠中,低硒,低碘对胫骨长度、生长板厚度、增殖细胞层数、肥大细胞层数影响明显(F值分别为24.31、6.98,40.76、56.15,25.24、82.82,10.07、5.57,P<0.01或<0.05).低硒和低碘对胫骨长度、生长板厚度、增殖细胞层数、肥大细胞层数的影响存在交互作用(F值分别为5.68、24.86、41.82、9.12,P<0.05或<0.01);低硒组[(33.17±0.34)mm]、低硒低碘组胫骨长度[(31.30±0.87)mm]明显低于对照组[(34.12±0.32)turn,P均<0.05];低硒低碘组生长板厚度[(1.60±0.18)mm、增殖细胞层数(8.54±0.81)、肥大细胞层数(4.95±0.37)明显低于低硒组[(3.03±0.10)mm、14.68±0.84、6.60±0.31]、低碘组[(2.90±0.09)mm,13.75±0.33、6.61±0.84]及对照组[(3.19±0.09)mm、14.94±0.36、6.64±0.26,P均<0.05)];低碘组生长板厚度、增殖细胞层数小于对照组(P<0.05).结论 低硒影响子代大鼠胫骨生长,低碘影响子代大鼠软骨细胞增殖,降低生长板厚度,低硒低碘联合显著影响子代大鼠骨、软骨的生长.
目的 研究低硒、低碘及其聯閤對親代和子代大鼠骨、軟骨生長的影響.方法 48隻斷乳SD健康大鼠,按體質量隨機分對照組、低硒組、低碘組和低硒低碘組,每組12隻.4組大鼠分彆採用人工配製的低硒、低碘、低硒低碘及含硒、碘量正常的飼料餵飼.大鼠飼養8週後(約3月齡)進行組內繁殖;親代大鼠餵飼至6月齡、子代大鼠餵飼至3月齡時,取親代和子代大鼠血樣,測定血清硒及T3、T4;取大鼠右側脛骨及左側膝關節.用遊標卡呎測定脛骨長度、中段額狀橫徑、脛骨上耑關節軟骨橫徑、縱徑;膝關節包埋切片後,光鏡下測定脛骨近耑生長闆厚度、生長闆軟骨肥大層、增殖層細胞層數.結果 低硒對親代和子代大鼠血清硒有明顯影響(F值分彆239.56、232.68,P均<0.05),對于代血清T4水平也有明顯影響(F=6.95,P<0.05);低碘對親代和子代大鼠血清T3、T4均有明顯影響(F值分彆為14.11、14.05,30.29、34.53,P<0.01),低硒、低碘聯閤對子代大鼠血清T4水平的影響存在交互作用(F=5.99,P<0.01).親代和子代大鼠血清硒,低硒組[(30.28±6.34)、(43.95±9.75)μg/L]、低硒低碘組[(30.33±5.18)、(35.40±3.06)μg/L]均明顯低于低碘組[(345.83±29.55)、(245.24±9.95)μg/L]、對照組[(358.64±30.50)、(236.50±9.75),P均<0.05].低硒低碘組[(0.55±0.05)、(0.88±0.14)mmol/L]親代和子代大鼠血清T3均明顯低于對照組[(0.75±0.08)、(1.26±0.26)mmol/L,P均<0.05],低碘組[(24.11±2.29)、(42.10±8.92)mmol/L]、低硒低碘組[(20.66±1.93)、(26.55±5.98)mmol/L]親代和子代大鼠血清T4均明顯低于對照組[(36.15±2.74)、(52.79±8.84)mmol/L]和低硒組[(28.12±3.33)、(52.02±11.99)mmol/L,P均<0.05];低硒低碘組子代大鼠血清T4明顯低于低碘組(P<0.05).子代大鼠中,低硒,低碘對脛骨長度、生長闆厚度、增殖細胞層數、肥大細胞層數影響明顯(F值分彆為24.31、6.98,40.76、56.15,25.24、82.82,10.07、5.57,P<0.01或<0.05).低硒和低碘對脛骨長度、生長闆厚度、增殖細胞層數、肥大細胞層數的影響存在交互作用(F值分彆為5.68、24.86、41.82、9.12,P<0.05或<0.01);低硒組[(33.17±0.34)mm]、低硒低碘組脛骨長度[(31.30±0.87)mm]明顯低于對照組[(34.12±0.32)turn,P均<0.05];低硒低碘組生長闆厚度[(1.60±0.18)mm、增殖細胞層數(8.54±0.81)、肥大細胞層數(4.95±0.37)明顯低于低硒組[(3.03±0.10)mm、14.68±0.84、6.60±0.31]、低碘組[(2.90±0.09)mm,13.75±0.33、6.61±0.84]及對照組[(3.19±0.09)mm、14.94±0.36、6.64±0.26,P均<0.05)];低碘組生長闆厚度、增殖細胞層數小于對照組(P<0.05).結論 低硒影響子代大鼠脛骨生長,低碘影響子代大鼠軟骨細胞增殖,降低生長闆厚度,低硒低碘聯閤顯著影響子代大鼠骨、軟骨的生長.
목적 연구저서、저전급기연합대친대화자대대서골、연골생장적영향.방법 48지단유SD건강대서,안체질량수궤분대조조、저서조、저전조화저서저전조,매조12지.4조대서분별채용인공배제적저서、저전、저서저전급함서、전량정상적사료위사.대서사양8주후(약3월령)진행조내번식;친대대서위사지6월령、자대대서위사지3월령시,취친대화자대대서혈양,측정혈청서급T3、T4;취대서우측경골급좌측슬관절.용유표잡척측정경골장도、중단액상횡경、경골상단관절연골횡경、종경;슬관절포매절편후,광경하측정경골근단생장판후도、생장판연골비대층、증식층세포층수.결과 저서대친대화자대대서혈청서유명현영향(F치분별239.56、232.68,P균<0.05),대우대혈청T4수평야유명현영향(F=6.95,P<0.05);저전대친대화자대대서혈청T3、T4균유명현영향(F치분별위14.11、14.05,30.29、34.53,P<0.01),저서、저전연합대자대대서혈청T4수평적영향존재교호작용(F=5.99,P<0.01).친대화자대대서혈청서,저서조[(30.28±6.34)、(43.95±9.75)μg/L]、저서저전조[(30.33±5.18)、(35.40±3.06)μg/L]균명현저우저전조[(345.83±29.55)、(245.24±9.95)μg/L]、대조조[(358.64±30.50)、(236.50±9.75),P균<0.05].저서저전조[(0.55±0.05)、(0.88±0.14)mmol/L]친대화자대대서혈청T3균명현저우대조조[(0.75±0.08)、(1.26±0.26)mmol/L,P균<0.05],저전조[(24.11±2.29)、(42.10±8.92)mmol/L]、저서저전조[(20.66±1.93)、(26.55±5.98)mmol/L]친대화자대대서혈청T4균명현저우대조조[(36.15±2.74)、(52.79±8.84)mmol/L]화저서조[(28.12±3.33)、(52.02±11.99)mmol/L,P균<0.05];저서저전조자대대서혈청T4명현저우저전조(P<0.05).자대대서중,저서,저전대경골장도、생장판후도、증식세포층수、비대세포층수영향명현(F치분별위24.31、6.98,40.76、56.15,25.24、82.82,10.07、5.57,P<0.01혹<0.05).저서화저전대경골장도、생장판후도、증식세포층수、비대세포층수적영향존재교호작용(F치분별위5.68、24.86、41.82、9.12,P<0.05혹<0.01);저서조[(33.17±0.34)mm]、저서저전조경골장도[(31.30±0.87)mm]명현저우대조조[(34.12±0.32)turn,P균<0.05];저서저전조생장판후도[(1.60±0.18)mm、증식세포층수(8.54±0.81)、비대세포층수(4.95±0.37)명현저우저서조[(3.03±0.10)mm、14.68±0.84、6.60±0.31]、저전조[(2.90±0.09)mm,13.75±0.33、6.61±0.84]급대조조[(3.19±0.09)mm、14.94±0.36、6.64±0.26,P균<0.05)];저전조생장판후도、증식세포층수소우대조조(P<0.05).결론 저서영향자대대서경골생장,저전영향자대대서연골세포증식,강저생장판후도,저서저전연합현저영향자대대서골、연골적생장.
Objective To study the effects of selenium deficiency,iodine deficiency and combined selenium and iodine deficiency on bone and cartilage growth in the parental and the first filial generation rats. Methods Forty-eight weanling healthy SD rats were randomly divided into selenium deficieney, iodine deficiency, combined selenium and iodine deficiency and control groups according to their body mass. These rats were fed with selenium deficiency, iodine deficiency, combined selenium and iodine deficiency, and normal fodder, respectively. The parental rats (about 3 months old) were mated in each group 8 weeks after the beginning of the experiment. Right tibias and left knee joints were collected when the parental generation rats were about 6 months and the first filial generation rats were about 3 months old. Tibial length, mid-shaft tibial diameter, and articular cartilage diameters of the right tibias were measured by vernier caliper. Left knee joints were embedded and cut into sections and the thickness of the growth plate cartilage, layers of proliferative and hypertrophic chondrocytes in growth plate cartilage were observed under the light microscope. Results The selenium deficiency had significant effect on serum selenium level of the parental and the first filial generation rats(F value were 239.56,232.68, P< 0.01), and also on serum T4 level of the first filial generation rats(F value were 6.95, P < 0.05). The iodine deficiency had significant effect on serum T3 and T4 level in the two generations rats(F value were 14.11,14.05,30.29,34.53, P < 0.01 ). There were interactions between selenium deficiency and iodine deficiency on serum T4 level in the first filial generation rats (F= 5.99, P< 0.05). The serum selenium of selenium deficiency group[ (30.28 ± 6.34), (43.95 ± 9.75)μg/L],combined selenium and iodine deficiency group[ (30.33 ± 5.18), (35.40 ± 3.16)μg/L] were significantly lower than iodine deficiency group[(345.83 ± 29.55), (245.24 ± 9.95)μg/L] and the controls[ (358.64 ± 30.50), (236.50 ±9.75) μg/L] in the two generations. The serum T3 of combined selenium and iodine deficiency group [(0.55 ± 0.05 ),(0.88 ± 0.14)nmol/L] were significantly lower than the controls[(0.75 ± 0.08), (1.26 ± 0.26)nmol/L] in the two generations. The serum T4 of iodine deficiency [ (24.11 ± 2.29), (42.10 ± 8.92) nmol/L ] and combined selenium and iodine deficiency group[ (20.66 ± 1.93), (26.55 ± 5.98)nmol/L] were significantly lower than the controls[ (36.15 ±2.74), (52.79 ± 8.84)nmol/L] and selenium deficiency group[ (28.12 ± 3.33), (52.02 ± ll.99)nmol/L] in the two generations. The selenium deficiency and iodine deficiency had significant effect on tibial length, thickness of the growth plate cartilage, layers of proliferative and hypertrophic chondrocytes in first filial generation rats(F values were 24.31,6.98,40.76,56.15,25.24,82.82, 10.07,5.57, P <0.05 or <0.01). There were interactions between selenium deficiency and iodine deficiency on tibial length, thickness of the growth plate cartilage, layers of proliferative and hypertrophic chondrocytes (F values were 5.68,24.86,41.82,9.12, P <0.05 or <0.01 ). The tibial length of the selenium deficiency group[ (33.17 ± 0.34)mm] and combined selenium and iodine deficiency group[ (31.30 ± 0.87)mm] were significantly lower than the controls[ (34.12 ± 0.32)mm, P< 0.05]. Thickness of the growth plate cartilage [ (1.60 ± 0.18)mm ], layers of proliferative chondrocyte (8.54 ± 0.81), and hypertrophic chondrocyte (4.95 ± 0.37)of the combined selenium and iodine deficiency group were significantly decreased when compared to the selenium deficiency group[ (3.03 ± 0.10)mm, 14.68 ± 0.84,6.60 ± 0.31], iodine deficiency group[ (2.90 ± 0.09)mm, 13.75 ±0.33,6.61 ± 0.84 ] and the controls [ (3.19 ± 0.09) mm, 14.94 ± 0.36, 6.64 ± 0.26, P <0.05]. Thickness of the growth plate cartilage, layers of proliferative chondrocyte of the iodine deficiency group were lower than the controls(P<0.05). Conclusions Selenium deficiency impair tibial growth in first filial generation rats, iodine deficiency retarded the chondroncyte proliferation and decreases the thickness of growth plate cartilage in first filial generation rats, and combined selenium and iodine deficiency significantly impair the growth of bone and cartilage in first filial generation rats.
