CAJ | 학술논문

目的探讨在自然衰老过程中血浆自身抗Aβ抗体的发生规律和对AD发病机制的启示.方法采用一种特殊的组织淀粉样蛋白免疫活性测定方法(TAPIR)检测205例年龄为1～91岁的健康人群血浆免疫染色Tg2576转基因小鼠大脑组织切片中的老年斑核心,随后血浆与人工合成的Aβ多肽(sAβ)、蚁酸可溶性淀粉样Aβ颗粒(FAβ)进行免疫沉降和免疫印迹,以探查血浆自身抗Aβ抗体的免疫特性.结果自然发生的自身抗Aβ抗体存在于各个年龄段,甚至于儿童和青少年,总发生率为35.6%,其变化规律呈现一种特殊的"U"型年龄相关性曲线.结论血浆自身抗Aβ抗体的出现先于脑内淀粉样斑块的形成,且与脑内Aβ负荷无关.
목적 탐토재자연쇠로과정중혈장자신항Aβ항체적발생규률화대AD발병궤제적계시.방법 채용일충특수적조직정분양단백면역활성측정방법(TAPIR)검측205례년령위1～91세적건강인군혈장면역염색Tg2576전기인소서대뇌조직절편중적노년반핵심,수후혈장여인공합성적Aβ다태(sAβ)、의산가용성정분양Aβ과립(FAβ)진행면역침강화면역인적,이탐사혈장자신항Aβ항체적면역특성.결과 자연발생적자신항Aβ항체존재우각개년령단,심지우인동화청소년,총발생솔위35.6%,기변화규률정현일충특수적"U"형년령상관성곡선.결론 혈장자신항Aβ항체적출현선우뇌내정분양반괴적형성,차여뇌내Aβ부하무관.
Objective To investigate the occurrence rates and changing role of plasma antibodies to Aβs in normal aging and their enlightenment to the pathogenesis of Alzheimer's disease.Methods Paraffin serial sections from Tg2576 mouse brains were immunostained with 205 plasma samples from healthy cases aged from 1 to 91 years old to make a specific tissue amyloid plaque immunoreactivity analysis, and subsequently, immunoprecipitation of plasma with synthetic Aβ peptides and formic acid soluble amyloid Aβ fractions (FAβ) was employed to clarify the immunospecialties of autoantibodies to Aβs. Results Plasma autoantibodies to Aβs were found to be naturally present in all age periods, even in childhood and juvenile, the total occurrence rate was 35.6%, and they showed a special age-related U-typed trend during aging. Conclusions Plasma autoantibodies to Aβ can occur before amyloid plaques in brains, and with no relationship with brain Aβ burden.