CAJ | 학술논문

目的研究人巨细胞病毒(HCMV)对人涎腺导管上皮细胞(HSG)细胞周期的影响及其相关机制.方法体外培养HSG;用RT-PCR及nest-RT-PCR法检测感染HCMV的HSG中立即早期基因(ie1/ie2)的转录;用流式细胞仪检测HCMV对HSG细胞周期的影响;用蛋白印迹技术检测HCMV感染细胞中周期素D1的表达.结果感染HCMV的HSG中可检测到HCMV ie1/ie2的转录;HCMV通过影响细胞周期的G1/S关卡,使HSG阻滞于G1期;感染HCMV的HSG周期素D1表达下调.结论在体外HCMV通过作用于细胞周期的G1/S关卡及下调周期素D1抑制涎腺导管上皮细胞的增殖.
목적 연구인거세포병독(HCMV)대인연선도관상피세포(HSG)세포주기적영향급기상관궤제.방법 체외배양HSG;용RT-PCR급nest-RT-PCR법검측감염HCMV적HSG중립즉조기기인(ie1/ie2)적전록;용류식세포의검측HCMV대HSG세포주기적영향;용단백인적기술검측HCMV감염세포중주기소D1적표체.결과 감염HCMV적HSG중가검측도HCMV ie1/ie2적전록;HCMV통과영향세포주기적G1/S관잡,사HSG조체우G1기;감염HCMV적HSG주기소D1표체하조.결론 재체외HCMV통과작용우세포주기적G1/S관잡급하조주기소D1억제연선도관상피세포적증식.
Objective To investigate the effects of human cytomegalovirus (HCMV) on the cell cycle of duct epithelial cell cultures of human salivary gland ( HSG) in vitro and relative mechanism. Methods HSG was cultured in vitro. Reverse transcriptase polymerase chain reaction (RT-PCR) and nestRT-PCR were used respectively to investigate ie1/ie2 transcription in HSG infected by human cytomegalovirus( HCMV). The effects of HCMV on the cell cycle of HSG were studied by flow cytometry in vitro. The expression of cyclin D1 in HSG infected by HCMV was detected by Western blotting. Results HCMV ie1/ie2 transcription could be detected in HSG infected by HCMV. HCMV arrested productively infected cells in G1 stage. And cyclin D1 was down-regulated in HCMV infected HSG. Conclusion HCMV inhibits proliferation of HSG by affecting G1/S check point and down-regulating cyclin D1 in vitro.