CAJ | 학술논문

采用电子显微镜技术,研究了苹果黑星病菌在苹果叶片上发育过程.扫描电子显微镜观察结果表明,接种后12 h分生孢子即可萌发并形成附着胞,统计结果显示其孢子萌发率在6 h和12 h分别为83%和95%,附着胞形成率在12 h和24 h分别为93%和95%.透射电子显微镜观察结果表明,黑星病菌侵入以后在寄主角质层下和表皮细胞之间扩展、定殖并可形成子座.接种后12d,病菌开始从子座上产生分生孢子梗和分生孢子,分生孢子梗顶端每产生一个单生的分生孢子就形成一个环痕并延伸其长度.分生孢子梗和分生孢子主要沿叶脉形成,在叶片上呈网状扩展,此时叶片表现明显的病害症状.
채용전자현미경기술,연구료평과흑성병균재평과협편상발육과정.소묘전자현미경관찰결과표명,접충후12 h분생포자즉가맹발병형성부착포,통계결과현시기포자맹발솔재6 h화12 h분별위83%화95%,부착포형성솔재12 h화24 h분별위93%화95%.투사전자현미경관찰결과표명,흑성병균침입이후재기주각질층하화표피세포지간확전、정식병가형성자좌.접충후12d,병균개시종자좌상산생분생포자경화분생포자,분생포자경정단매산생일개단생적분생포자취형성일개배흔병연신기장도.분생포자경화분생포자주요연협맥형성,재협편상정망상확전,차시협편표현명현적병해증상.
Development of apple scab pathogen, Venturia inaequalis, on the leaves were examined bymeans of scanning and transmission electron microscopy. The results showed that the conidia of V.inaequalis germinated and developed appressoria within 12 h after inoculation. The germination percentagesof conidia examined at 6 h and 12 h after inoculation were 83% and 95% respectively. The frequency ofappressorium formation of the germinated conidia were 93% and 95% 12 h and 24 h after inoculationrespectively. The TEM observations revealed that after penetration of the cuticle growth of the pathogen wasentirely subcuticular during the stages of parasitism. The sporulation of the fungus started with theformation of conidia 12 days after inoculation in the apple leaves. With the initiation of the conidiogenesisthe enlarged stroma forced the cuticle outward until rupture. Each new proliferation lengthens the annellideand normally produces a single terminal conidium. Production of annelids and conidia occurs in narrowthread-like lines which radiate irregularly from one or more centers of origin (site of initial infections) andbranch repeatedly, forming a network over the leaf surface, often paralleling a leaf vein. The typical scabsymptom was observed at this sampling time.