国际检验医学杂志
國際檢驗醫學雜誌
국제검험의학잡지
INTERNATIONAL JOURNAL OF LABORATORY MEDICINE
2009年
4期
328-330,333
,共4页
邹义春%汪宏良%罗卓跃%骆明波%柯俊
鄒義春%汪宏良%囉卓躍%駱明波%柯俊
추의춘%왕굉량%라탁약%락명파%가준
假单胞菌,铜绿%抗药性,多药%感染
假單胞菌,銅綠%抗藥性,多藥%感染
가단포균,동록%항약성,다약%감염
Pseudomonas,aeruginosa%Drug resistance,multiple%Infection
目的 了解多药耐药铜绿假单胞菌(multi-drug-resistant pseudomonas aeruginosa.MDRPA)分离株亲缘性.方法 采用PCR检测20株MDRPA菌34种耐药基因,并作聚类分析.结果 20株MDRPA菌中,TEM、OXA-2群、OXA-10群、CARB、rmtB、aac(3)-Ⅱ、aac(6')-Ⅰ b、aac(6')-Ⅱ、ant(3")-Ⅰ、ant(2")-Ⅰ、cmlA、merA、qacE△1-sull基因阳性率分别为30%、5%、5%、25%、35%,10%、25%、30%、35%、15%、30%、35%、50%,oprD2缺失率为100%.聚类分析显示存在克隆传播现象.结论 TEM、CARB、rmtB、aac(3)-Ⅱ、aac(6])-Ⅰ b、aac(6')-Ⅱ、ant(3")-Ⅰ、ant(2")-Ⅰ、cmlA,merA、qacE△1-sull基因检出率高,MDRPA菌可导致克隆传播医院内感染.
目的 瞭解多藥耐藥銅綠假單胞菌(multi-drug-resistant pseudomonas aeruginosa.MDRPA)分離株親緣性.方法 採用PCR檢測20株MDRPA菌34種耐藥基因,併作聚類分析.結果 20株MDRPA菌中,TEM、OXA-2群、OXA-10群、CARB、rmtB、aac(3)-Ⅱ、aac(6')-Ⅰ b、aac(6')-Ⅱ、ant(3")-Ⅰ、ant(2")-Ⅰ、cmlA、merA、qacE△1-sull基因暘性率分彆為30%、5%、5%、25%、35%,10%、25%、30%、35%、15%、30%、35%、50%,oprD2缺失率為100%.聚類分析顯示存在剋隆傳播現象.結論 TEM、CARB、rmtB、aac(3)-Ⅱ、aac(6])-Ⅰ b、aac(6')-Ⅱ、ant(3")-Ⅰ、ant(2")-Ⅰ、cmlA,merA、qacE△1-sull基因檢齣率高,MDRPA菌可導緻剋隆傳播醫院內感染.
목적 료해다약내약동록가단포균(multi-drug-resistant pseudomonas aeruginosa.MDRPA)분리주친연성.방법 채용PCR검측20주MDRPA균34충내약기인,병작취류분석.결과 20주MDRPA균중,TEM、OXA-2군、OXA-10군、CARB、rmtB、aac(3)-Ⅱ、aac(6')-Ⅰ b、aac(6')-Ⅱ、ant(3")-Ⅰ、ant(2")-Ⅰ、cmlA、merA、qacE△1-sull기인양성솔분별위30%、5%、5%、25%、35%,10%、25%、30%、35%、15%、30%、35%、50%,oprD2결실솔위100%.취류분석현시존재극륭전파현상.결론 TEM、CARB、rmtB、aac(3)-Ⅱ、aac(6])-Ⅰ b、aac(6')-Ⅱ、ant(3")-Ⅰ、ant(2")-Ⅰ、cmlA,merA、qacE△1-sull기인검출솔고,MDRPA균가도치극륭전파의원내감염.
Objective To investigate the affinity of muhidrug resistant isolates of Pseudomonas aeruginosa. Methods The 34 kinds of drug resistance genes of 20 strains of multidrug resistant Pseud-omonas aeruginosa (MDRPA) were detected by applying polymerase chain reaction (PCR) and cluster analysis. Results The positive rates of TEM, OXA-2group, OXA-10group, CARB, rmtB, aac(3)- Ⅱ , aac(6')- Ⅰ b,aac(6')- Ⅱ ,ant(3"-Ⅰ ,ant(2")- Ⅰ ,cmlA,merA,qacE△l-sull were 30% ,5% ,5% ,25%, 35%, 10% ,25% ,30% ,35%, 15%,30% ,35% and 50%, respectively. The deletion rate of oprD2 gene was 100%. Cluster analysis showed that the clone transmission occurred. Conclusion There are very high detection rates of TEM,CARB,rmtB,aac(3)- Ⅱ ,aac(6')- Ⅰ b,aac(6')- Ⅱ ,ant(3")- Ⅰ ,ant(2")- Ⅰ, cmlA,merA,qacE△l-sull genes in MDRPA. The MDRPA can induce clone transmitted hospital infec-tion.
