CAJ | 학술논문

目的对比观察针刀松解法、电针对膝骨关节炎兔中枢神经5-羟色胺类和儿茶酚胺类神经递质的影响.方法采用高效液相色谱仪分别测定端脑、下丘脑、脑干和脊髓中5-羟色胺(5-HT)、5-羟吲哚乙酸(5-HIAA)、去甲肾上腺素(NA)、高香草酸(HVA)和3,4-二羟基苯乙酸(DOPAC)含量.结果模型组端脑、下丘脑、脑干和脊髓等的5-HT含量与正常组相比均无显著性差异,但有降低的趋势;模型组下丘脑和脑干中5-HIAA含量与正常组相比无显著性差异,但端脑和腰膨大5-HIAA含量较正常组显著降低,针刀治疗后端脑或脊髓5-HIAA含量与模型组相比显著升高;电针治疗后端脑5-HIAA含量与模型组相比显著升高,而脊髓中5-HIAA含量与模型组相比显著降低.模型组端脑NA含量较正常组显著降低;针刀或电针治疗后NA含量与模型组相比较呈显著性升高;模型组下丘脑和脑干NA含量较正常组显著性增加,针刀或电针治疗后显著降低;模型组脊髓NA含量与正常组相比较无显著差别.模型组端脑HVA含量较正常组显著降低,针刀或电针治疗后显著性升高,针刀组与电针组相比较无显著性差别;模型组下丘脑、脑干和脊髓HVA含量与正常组比较无显著差别.模型组端脑和脊髓DOPAC含量与正常组相比无显著变化;模型组下丘脑和脑干DOPAC含量较正常组明显升高,针刀或电针治疗后DOPAC含量较模型组均显著降低.结论针刀松解法或电针疗法对膝骨关节炎兔脑、脊髓中5-羟色胺类和儿茶酚胺类神经递质的含量有一定的调节作用,提示2种疗法在减轻关节软骨损伤的同时,可通过调节中枢神经5-羟色胺和儿茶酚胺类神经递质的合成和代谢以缓解关节疼痛的发生和发展.
목적 대비관찰침도송해법、전침대슬골관절염토중추신경5-간색알류화인다분알류신경체질적영향.방법 채용고효액상색보의분별측정단뇌、하구뇌、뇌간화척수중5-간색알(5-HT)、5-간신타을산(5-HIAA)、거갑신상선소(NA)、고향초산(HVA)화3,4-이간기분을산(DOPAC)함량.결과 모형조단뇌、하구뇌、뇌간화척수등적5-HT함량여정상조상비균무현저성차이,단유강저적추세;모형조하구뇌화뇌간중5-HIAA함량여정상조상비무현저성차이,단단뇌화요팽대5-HIAA함량교정상조현저강저,침도치료후단뇌혹척수5-HIAA함량여모형조상비현저승고;전침치료후단뇌5-HIAA함량여모형조상비현저승고,이척수중5-HIAA함량여모형조상비현저강저.모형조단뇌NA함량교정상조현저강저;침도혹전침치료후NA함량여모형조상비교정현저성승고;모형조하구뇌화뇌간NA함량교정상조현저성증가,침도혹전침치료후현저강저;모형조척수NA함량여정상조상비교무현저차별.모형조단뇌HVA함량교정상조현저강저,침도혹전침치료후현저성승고,침도조여전침조상비교무현저성차별;모형조하구뇌、뇌간화척수HVA함량여정상조비교무현저차별.모형조단뇌화척수DOPAC함량여정상조상비무현저변화;모형조하구뇌화뇌간DOPAC함량교정상조명현승고,침도혹전침치료후DOPAC함량교모형조균현저강저.결론 침도송해법혹전침요법대슬골관절염토뇌、척수중5-간색알류화인다분알류신경체질적함량유일정적조절작용,제시2충요법재감경관절연골손상적동시,가통과조절중추신경5-간색알화인다분알류신경체질적합성화대사이완해관절동통적발생화발전.
Objective To compare and observe the effects of acupotome dissolution therapy and electro- acupuncture on central neurotransmitters of 5-hyproxytryptamines (5-HT) and catecholamine (CA) in rabbits with knee osteoarthritis. Method The content of 5-HT, 5-hydroxyindole acetic acid (5-HIAA), noradrenaline (NA), homovanillic acid (HVA) and 3, 4-dihydroxyphenylacetic acid (DOPAC) in the endbrain, hypothalamus, brain stem and spinal cord were determined by applying HPLC. Result There was no significant difference but a downward trend in the content of 5-HT in the endbrain, hypothalamus, brain stem and spinal cord between the model group and normal group. There was no significant difference in the content of 5-HIAA in the hypothalamus and brain stem between the model group and normal group. The content of 5-HIAA in the endbrain and spinal cord decreased significantly in the model group compared with that in the normal group, increased significantly in the acupotome group compared with that in the model group, increased significantly in the endbrain in the electro-acupuncture group compared with that in the model group, and decreased significantly in the spinal cord in the electro- acupuncture group compared with that in the model group. The content of NA in the endbrain decreased significantly compared with that in the normal group, increased significantly in the acupotome group or electro-acupuncture group compared with that in the model group, increased significantly in the hypothalamus and brain stem in the model group compared with that in the normal group, and decreased significantly in the acupotome group or electro-acupuncture group. There was no significant difference in NA content in the spinal cord between the model group and normal group. The content of HVA in the endbrain decreased significantly in the model group compared with that in the normal group, and increased significantly in knife group or electro-acupuncture group. There was no significant difference in HVA content in the endbrain between the acupotome group and electro- acupuncture group. There was no significant difference in HVA content in the hypothalamus, brain stem or spinal cord between the model group and normal group. The content of DOPAC in the endbrain or spinal cord had no significant changes in the model group compared with that in the normal group, increased significantly in the hypothalamus or brain stem in the model group compared with that in the normal group, and decreased significantly in the acupotome group or electro-acupuncture group compared with that in the model group. Conclusion The acupotome dissolution therapy and electro-acupuncture can regulate the content of neurotransmitters of 5-HT and CA in the brain and spinal cord in rabbits with knee osteoarthritis, which indicates that the two therapies can relieve the injury to the articular cartilage, as well as abates joint pain and stops osteoarthritis development based on the regulation of the synthesis and metabolism of neurotransmitters in the central nervous system.