浙江农业学报
浙江農業學報
절강농업학보
ACTA AGRICULTURAE ZHEJIANGENSIS
2010年
1期
1-5
,共5页
水稻%矮化多分蘖突变基因%遗传分析%图位克隆
水稻%矮化多分蘗突變基因%遺傳分析%圖位剋隆
수도%왜화다분얼돌변기인%유전분석%도위극륭
rice(Orym sativa L.)%high-tillering and dwarf mutant%genetic analysis%map-based cloning
从粳稻品种'中花11'转基因后代中发现了一个矮秆多分蘖突变体,突变体表现出植株矮化、分蘖力强、穗长变短、叶片变窄及结实率降低等一系列突变表型.遗传分析表明该矮秆多分蘖突变体表型受一对隐性核基因控制,因其与突变体htd1具有相类似的表型,故将该基因命名为htd2.利用籼粳杂交F_2群体将突变基因定位在第3染色体短臂上SSR标记RM6038和RM5444之间,与两个标记的遗传距离分别为1.4和2.1 cM,两个标记之间的物理距离大约为400 kb.
從粳稻品種'中花11'轉基因後代中髮現瞭一箇矮稈多分蘗突變體,突變體錶現齣植株矮化、分蘗力彊、穗長變短、葉片變窄及結實率降低等一繫列突變錶型.遺傳分析錶明該矮稈多分蘗突變體錶型受一對隱性覈基因控製,因其與突變體htd1具有相類似的錶型,故將該基因命名為htd2.利用秈粳雜交F_2群體將突變基因定位在第3染色體短臂上SSR標記RM6038和RM5444之間,與兩箇標記的遺傳距離分彆為1.4和2.1 cM,兩箇標記之間的物理距離大約為400 kb.
종갱도품충'중화11'전기인후대중발현료일개왜간다분얼돌변체,돌변체표현출식주왜화、분얼력강、수장변단、협편변착급결실솔강저등일계렬돌변표형.유전분석표명해왜간다분얼돌변체표형수일대은성핵기인공제,인기여돌변체htd1구유상유사적표형,고장해기인명명위htd2.이용선갱잡교F_2군체장돌변기인정위재제3염색체단비상SSR표기RM6038화RM5444지간,여량개표기적유전거리분별위1.4화2.1 cM,량개표기지간적물리거리대약위400 kb.
A rice mutant,showing the phenotype of high-tillering and dwarfing,was discovered from a population derived from a transgenic japonica cuhivar'Zhonghua1 1'.The genetic analysis indicated that the phenotype of the mutant was controlled by a single recessive gene.which was designated tentatively as htd2.The F_2 population Wag developed from a cross between the mutant and an indica cultivar'Longtefu B'and used to investigate the genetic model and map the gene.The hid2 gene wag firstly mapped on the short arm of chromosome3 and further mapped on the genetic region between SSR marker RM6038 and RM5444.which wag 400 kb in length.