中南大学学报（医学版）
中南大學學報（醫學版）
중남대학학보（의학판）
JOURNAL OF CENTRAL SOUTH UNIVERSITY (MEDICAL SCIENCES)
2010年
11期
1123-1128
,共6页
罗树伟%林戈%孙真%谢平原%刘天成%卢光琇
囉樹偉%林戈%孫真%謝平原%劉天成%盧光琇
라수위%림과%손진%사평원%류천성%로광수
人类胚胎干细胞%饲养细胞%自分泌
人類胚胎榦細胞%飼養細胞%自分泌
인류배태간세포%사양세포%자분비
human embryonic stem cells%feeder cells%autocrine
目的:建立人类胚胎干细胞(human embryonic stem cells,hESCs)无饲养细胞无外源性细胞因子体外培养体系.方法:比较hESCs克隆种植密度对其无饲养细胞无外源性细胞因子体外培养的影响,以及克隆种植密度对hESCs培养体系骨形蛋白(bone morphology protein,BMP)样诱导活性的影响.结果:在没有外源性细胞因子条件下,高克隆密度(34克隆/cm2)可以维持hESCs处于未分化状态,而低克隆密度不能维持hESCs处于未分化状态.同时,NB体系BMP样诱导活性可以被hESCs克隆种植密度所改变,高hESCs克隆密度时,培养基具有高的BMP样诱导活性.结论:高hESCs克隆种植密度可以改变自身生长的微环境,可以维持自身未分化状态,这为阐明hESCs自身未分化状态机制和简化hESCs无饲养培养体系提供了新的思路.
目的:建立人類胚胎榦細胞(human embryonic stem cells,hESCs)無飼養細胞無外源性細胞因子體外培養體繫.方法:比較hESCs剋隆種植密度對其無飼養細胞無外源性細胞因子體外培養的影響,以及剋隆種植密度對hESCs培養體繫骨形蛋白(bone morphology protein,BMP)樣誘導活性的影響.結果:在沒有外源性細胞因子條件下,高剋隆密度(34剋隆/cm2)可以維持hESCs處于未分化狀態,而低剋隆密度不能維持hESCs處于未分化狀態.同時,NB體繫BMP樣誘導活性可以被hESCs剋隆種植密度所改變,高hESCs剋隆密度時,培養基具有高的BMP樣誘導活性.結論:高hESCs剋隆種植密度可以改變自身生長的微環境,可以維持自身未分化狀態,這為闡明hESCs自身未分化狀態機製和簡化hESCs無飼養培養體繫提供瞭新的思路.
목적:건립인류배태간세포(human embryonic stem cells,hESCs)무사양세포무외원성세포인자체외배양체계.방법:비교hESCs극륭충식밀도대기무사양세포무외원성세포인자체외배양적영향,이급극륭충식밀도대hESCs배양체계골형단백(bone morphology protein,BMP)양유도활성적영향.결과:재몰유외원성세포인자조건하,고극륭밀도(34극륭/cm2)가이유지hESCs처우미분화상태,이저극륭밀도불능유지hESCs처우미분화상태.동시,NB체계BMP양유도활성가이피hESCs극륭충식밀도소개변,고hESCs극륭밀도시,배양기구유고적BMP양유도활성.결론:고hESCs극륭충식밀도가이개변자신생장적미배경,가이유지자신미분화상태,저위천명hESCs자신미분화상태궤제화간화hESCs무사양배양체계제공료신적사로.
Objective To establish human embryonic stem cells (hESCs) feeder-independent and cell factor-free culture system. Methods Effect of high and low clone densities of hESCs culture was compared and impact of the clone densities to hESCs culture media was analyzed. Results HESCs could maintain their undifferentiated states at high clone density (34 clones/cm2) without cell factors. At the same time,the bone morphology protein (BMP)-like induction of N2 and B27 supplements (NB) medium could be modulated by the clone density,and high level of BMP-like induction was accompanied by high clone density. Conclusion High clone density of hESCs can change the environments by themselves to maintain the undifferentiated states,which provides a new clue to explore the mechanism of undifferentiated states of hESCs and simplify the culture medium.
