CAJ | 학술논문

目的:胃祺饮是对慢性萎缩性胃炎具有良好临床疗效的中药复方.本实验研究胃祺饮挥发油的主要成分及其对AGS细胞增殖的抑制作用.方法:采用气相色谱-质谱联用法测定胃祺饮全方挥发油的化学成分,采用四甲基偶氮唑蓝法测定细胞活性,流式细胞分析检测细胞周期分布,使用Hoechst 33342和碘化丙啶双染法测定细胞凋亡和坏死.结果:化学分析显示胃祺饮挥发油的主要成分是冰片烯和3-正丁基苯酞.当归的有效成分藁本内酯在胃祺饮挥发油中没有检测到.胃祺饮挥发油能呈剂量和时间依赖性地抑制胃癌细胞的增殖,阻滞细胞周期于G2/M期,并能够诱导细胞的凋亡和坏死.结论:胃祺饮挥发油含有多种对于慢性萎缩性胃炎和功能性消化不良具有良好疗效的化学成分,具有显著的抗AGS细胞增殖的作用,其作用通过细胞周期阻滞和诱导细胞凋亡实现.因此在胃祺饮的煎煮过程中,应尽量避免挥发油的损失.
목적:위기음시대만성위축성위염구유량호림상료효적중약복방.본실험연구위기음휘발유적주요성분급기대AGS세포증식적억제작용.방법:채용기상색보-질보련용법측정위기음전방휘발유적화학성분,채용사갑기우담서람법측정세포활성,류식세포분석검측세포주기분포,사용Hoechst 33342화전화병정쌍염법측정세포조망화배사.결과:화학분석현시위기음휘발유적주요성분시빙편희화3-정정기분태.당귀적유효성분고본내지재위기음휘발유중몰유검측도.위기음휘발유능정제량화시간의뢰성지억제위암세포적증식,조체세포주기우G2/M기,병능구유도세포적조망화배사.결론:위기음휘발유함유다충대우만성위축성위염화공능성소화불량구유량호료효적화학성분,구유현저적항AGS세포증식적작용,기작용통과세포주기조체화유도세포조망실현.인차재위기음적전자과정중,응진량피면휘발유적손실.
Objective: The main ingredients and the inhibitory effects of essential oil of a compound Chinese herbal medicine Weiqi Decoction (WQD) on AGS cell proliferation were to be investigated. Methods: Chemical compounds of WQD essential oil were detected by gas chromatography and mass spectrometry analysis. Cell viability was measured by methyl thiazolyl tetrazolium method. Cell cycle distribution was detected by flow cytometry. Apoptosis and necrosis of AGS cells were determined by Hoechst 33342/propidium iodine staining. Results: Chemical analysis showed that the main ingredients of WQD essential oil were bornylene and 3-n-butylphthalide. Ligustilide, which is the effective compound of Danggui (Radix Angelicae Sinensis), was not detected in WQD essential oil. The essential oil inhibited cell proliferation in a dose- and time-dependent manner, and blocked cell cycle progression at G2/M stage. At the concentrations that resulted in significant inhibition of cell proliferation and cell cycle arrest, essential oil induced both apoptosis and necrosis. Conclusion: The results suggest that WQD essential oil contains some effective ingredients for treating chronic atrophic gastritis and functional dyspepsia, and also has an antiproliferative effect on AGS cells through cell cycle arrest and apoptosis promotion in vitro. Therefore, essential oil should be retained as much as possible during stewing this decoction.