CAJ | 학술논문

目的观察赤芍煎剂对急性胰腺炎(acute pancreatitis,AP)模型大鼠的治疗效果及对核因子kappaB (NF-κB)、肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)表达的影响.方法 SD大鼠40只采用完全随机分组方法分为5组:正常对照组(A组)、AP模型24 h组(B组)、AP模型72 h组(C组)、24 h赤芍煎剂组(D组)、72 h赤芍煎剂组(E组),每组8只.采用L-精氨酸腹腔注射诱发急性胰腺炎,造模后分别给予生理盐水(B、C组)、赤芍煎剂(D、E组)灌胃,于造模后24、72 h点处死各组大鼠.采血检测血清淀粉酶、TNF-α、IL-6；取胰腺组织分为2份:HE染色行光镜检查、Western Blot法检测NF-κB p65蛋白表达.结果与A组比较,B、D组大鼠血清淀粉酶均升高[分别为(3569±516) U/L、(2502±385) U/L,P＜0.05].但D组的血清淀粉酶水平明显低于B组(P＜0.05).C、E组血清淀粉酶水平均降低,接近A组[分别为(1279±142) U/L、(1421±203) U/L,P＞o.05].D组与B组比较、E组与C组比较,病理改变和病理学评分均减轻.血清TNF-α、IL-6的水平:D组为(12.54±2.12) pg/ml、(552.88±81.27)pg/ml,明显低于B组(20.53±4.16) pg/ml、(1255.75±l18.82)pg/ml;E组为(11.01±2.02) pg/ml、(582.87±88.86) pg/ml,明显低于C组(23.85±5.59) pg/ml、(1366.33±163.16) pg/ml.Western Blot结果显示,D组的NF-κB p65/β-actin比值(0.07±0.055)低于B组(0.71±0.029),E组(0.15±0.038)低于C组(0.65±0.026).结论赤芍煎剂对AP模型大鼠的作用机制可能与其抑制NF-κB活化、在整体水平上抑制细胞因子基因表达有关.
목적 관찰적작전제대급성이선염(acute pancreatitis,AP)모형대서적치료효과급대핵인자kappaB (NF-κB)、종류배사인자-α(TNF-α)、백세포개소-6(IL-6)표체적영향.방법 SD대서40지채용완전수궤분조방법분위5조:정상대조조(A조)、AP모형24 h조(B조)、AP모형72 h조(C조)、24 h적작전제조(D조)、72 h적작전제조(E조),매조8지.채용L-정안산복강주사유발급성이선염,조모후분별급여생리염수(B、C조)、적작전제(D、E조)관위,우조모후24、72 h점처사각조대서.채혈검측혈청정분매、TNF-α、IL-6；취이선조직분위2빈:HE염색행광경검사、Western Blot법검측NF-κB p65단백표체.결과 여A조비교,B、D조대서혈청정분매균승고[분별위(3569±516) U/L、(2502±385) U/L,P＜0.05].단D조적혈청정분매수평명현저우B조(P＜0.05).C、E조혈청정분매수평균강저,접근A조[분별위(1279±142) U/L、(1421±203) U/L,P＞o.05].D조여B조비교、E조여C조비교,병리개변화병이학평분균감경.혈청TNF-α、IL-6적수평:D조위(12.54±2.12) pg/ml、(552.88±81.27)pg/ml,명현저우B조(20.53±4.16) pg/ml、(1255.75±l18.82)pg/ml;E조위(11.01±2.02) pg/ml、(582.87±88.86) pg/ml,명현저우C조(23.85±5.59) pg/ml、(1366.33±163.16) pg/ml.Western Blot결과현시,D조적NF-κB p65/β-actin비치(0.07±0.055)저우B조(0.71±0.029),E조(0.15±0.038)저우C조(0.65±0.026).결론 적작전제대AP모형대서적작용궤제가능여기억제NF-κB활화、재정체수평상억제세포인자기인표체유관.
Objective To observe the therapeutic effect of red peony root decoction (RPRD) on model rats with acute pancreatitis (AP),and the effect on the expression of NF-κB,TNF-α and IL-6.Methods Forty SD rats were randomly divided into five groups:normal control group (group A),AP model groups (group B,24 h; group C,72 h) and RPRD treatment groups (group D,24 h; group E,72 h).There were 8 rats in each group.The AP models were induced by intraperitoneally injecting with L-arginine.After the model was established,rats of group B and group C were treated with intra-gastric instillation of NS,and rats in group D and group E with RPRD.At 24 and 72 hours after induction of AP,rats in each group were sacrificed and blood samples were taken for the measurement of serum amylase,TNF-α and IL-6.The expression of NF-κB p65 protein in the pancreas were detected by Western Blot,and pathological status of the pancreas were analyzed.Results The serum amylase level in group B and group D was significantly higher than that in group A (P＜0.05),and the level in group D was obviously lower than that in group B (P＜0.05).The serum amylase level in group C and group E was decreased,and there was no difference between group A (P＞0.05).The pathological severity and pathological scores of the pancreas in group D and group E were more lighter than those in group B and group C (P＜0.05).The level of serum TNF-α and IL-6 was significantly decreased in RPRD treatment groups (P＜0.05),group D compared with group B,[ (12.54±2.12)pg/ml,(552.88±81.27)pg/ml] vs.[ (20.53±4.16)pg/ml,(1255.75± 118.82)pg/ml]; group E compared with group C,[ (11.01 ±2.02)pg/ml,(582.87± 88.86) pg/ml] vs [ (23.85 ± 5.59) pg/ml,(1366.33 ± 163.16) pg/ml].The expression levels of NF-κBp65 protein in the pancreas of group D and group E were significantly lighter than those in group C at both time points (group D compared with group B,(0.07±0.055) vs (0.71 ±0.029),P＜0.05; group E compared with group C,(0.15±0.038) vs (0.65±0.026).Conclusion RPRD may alleviate the pathologic changes in AP