CAJ | 학술논문

目的寻找制作高稳定性大鼠大脑中动脉闭塞(middle cerebral artery occlusion,MCAO)模型和无创性评价模型效果的方法.方法采用6种不同直径的栓线制作大鼠MCAO模型并比较其成功率、蛛网膜下腔出血发生率以及梗死体积,在术后和再灌注24 h进行模型评分以评价模型的效果.结果在使用直径为0.28 mm和头端直径为0.32～0.34 mm的栓线时,模型成功率较高,蛛网膜下腔出血发生率较低,再灌注后24 h进行模型评分的敏感性和特异性较高.结论使用直径为0.28 mm和头端直径为0.32～0.34 mm的栓线能提高模型的稳定性,在再灌注后24 h进行模型评分能无创性评价模型效果.
목적 심조제작고은정성대서대뇌중동맥폐새(middle cerebral artery occlusion,MCAO)모형화무창성평개모형효과적방법.방법 채용6충불동직경적전선제작대서MCAO모형병비교기성공솔、주망막하강출혈발생솔이급경사체적,재술후화재관주24 h진행모형평분이평개모형적효과.결과 재사용직경위0.28 mm화두단직경위0.32～0.34 mm적전선시,모형성공솔교고,주망막하강출혈발생솔교저,재관주후24 h진행모형평분적민감성화특이성교고.결론 사용직경위0.28 mm화두단직경위0.32～0.34 mm적전선능제고모형적은정성,재재관주후24 h진행모형평분능무창성평개모형효과.
Objective To search for the methods of inducing the high stability rat middle cerebral artery occlusion (MCAO) model and to noninvasively assess the method of model effect. Methods Six kinds of filaments with different diameters were used to induce rat MCAO models and their success rate, incidence of subarachnoid hemorrhage, and infarct volume were compared. The model scores were performed to assess the model effect after the operation and at 24 hours after reperfusion. Results Higher model success rate and lower incidence of subarachnoid hemorrhage were achieved when the 0. 28 mm in diameter and 0.32-0.34 mm in tip diameter filaments were used. 1he sensibility and specificity were higher when the model scores were performed at 24 hours after reperfusion. Conclusions Using the filaments of 0.28 mm in diameter and 0. 32-0. 34 mm in tip diameter for intraluminal thread could improved the stability of the models. The model scores at 24 hours after reperfusion could noninvasively assess the model effect.