中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2012年
6期
759-762
,共4页
娄邵菲%张川%曹景朝%侯桂荚%叶明%赵亚昆
婁邵菲%張川%曹景朝%侯桂莢%葉明%趙亞昆
루소비%장천%조경조%후계협%협명%조아곤
西罗莫司/药理学%前列腺肿瘤/代谢/病理学%细胞凋亡%肿瘤转移
西囉莫司/藥理學%前列腺腫瘤/代謝/病理學%細胞凋亡%腫瘤轉移
서라막사/약이학%전렬선종류/대사/병이학%세포조망%종류전이
Sirolimus/pharmacology%Prostatic neoplasms/metabolism/pathology%Apoptosis%Neoplasm metastasis
目的 观察雷帕霉素(RPM)对人前列腺癌细胞PC-3生长、周期、凋亡及转移的影响,探讨RPM抑制前列腺癌细胞生长、转移的可能机制及临床应用前景.方法 体外培养前列腺癌PC-3细胞,用不同浓度的RPM( 10、25 ng/ml)干预Pc-3细胞.采用MTI法检测RPM对于PC-3细胞增殖的影响;流式细胞仪检测PC-3细胞周期及凋亡的变化;动物体内荷瘤实验检测对PC-3侵袭转移的影响.结果 RPM能显著抑制PC-3细胞生长增殖,且呈时间和浓度依赖性,在25 ng/ml、36h时抑制率达到(42.23±0.78)％,差异有统计学意义(P＜0.05);流式细胞分析显示RPM能显著抑制细胞周期,使Go/G1期细胞增多(P＜0.05),在25 ng/ml、36 h时Go/G1期细胞达到(92.17±0.69)％,并促进肿瘤细胞的早期凋亡(P＜0.05),在25 ng/ml、36 h时凋亡率达到(28.75±1.31)％;动物体内荷瘤实验较对照组差别明显,对照组肿瘤重量与转移比率明显高于RPM组[(3.41±0.28)g vs (1.19+0.23)g,100％ (7/7) vs 14.29％ (1/7),P＜0.05],肿瘤增长和转移被显著抑制.结论 RPM明显抑制人前列腺癌细胞PC-3的生长及迁移,以RPM为基础的前列腺癌治疗方案可能在临床中具有良好的应用前景.
目的 觀察雷帕黴素(RPM)對人前列腺癌細胞PC-3生長、週期、凋亡及轉移的影響,探討RPM抑製前列腺癌細胞生長、轉移的可能機製及臨床應用前景.方法 體外培養前列腺癌PC-3細胞,用不同濃度的RPM( 10、25 ng/ml)榦預Pc-3細胞.採用MTI法檢測RPM對于PC-3細胞增殖的影響;流式細胞儀檢測PC-3細胞週期及凋亡的變化;動物體內荷瘤實驗檢測對PC-3侵襲轉移的影響.結果 RPM能顯著抑製PC-3細胞生長增殖,且呈時間和濃度依賴性,在25 ng/ml、36h時抑製率達到(42.23±0.78)％,差異有統計學意義(P＜0.05);流式細胞分析顯示RPM能顯著抑製細胞週期,使Go/G1期細胞增多(P＜0.05),在25 ng/ml、36 h時Go/G1期細胞達到(92.17±0.69)％,併促進腫瘤細胞的早期凋亡(P＜0.05),在25 ng/ml、36 h時凋亡率達到(28.75±1.31)％;動物體內荷瘤實驗較對照組差彆明顯,對照組腫瘤重量與轉移比率明顯高于RPM組[(3.41±0.28)g vs (1.19+0.23)g,100％ (7/7) vs 14.29％ (1/7),P＜0.05],腫瘤增長和轉移被顯著抑製.結論 RPM明顯抑製人前列腺癌細胞PC-3的生長及遷移,以RPM為基礎的前列腺癌治療方案可能在臨床中具有良好的應用前景.
목적 관찰뢰파매소(RPM)대인전렬선암세포PC-3생장、주기、조망급전이적영향,탐토RPM억제전렬선암세포생장、전이적가능궤제급림상응용전경.방법 체외배양전렬선암PC-3세포,용불동농도적RPM( 10、25 ng/ml)간예Pc-3세포.채용MTI법검측RPM대우PC-3세포증식적영향;류식세포의검측PC-3세포주기급조망적변화;동물체내하류실험검측대PC-3침습전이적영향.결과 RPM능현저억제PC-3세포생장증식,차정시간화농도의뢰성,재25 ng/ml、36h시억제솔체도(42.23±0.78)％,차이유통계학의의(P＜0.05);류식세포분석현시RPM능현저억제세포주기,사Go/G1기세포증다(P＜0.05),재25 ng/ml、36 h시Go/G1기세포체도(92.17±0.69)％,병촉진종류세포적조기조망(P＜0.05),재25 ng/ml、36 h시조망솔체도(28.75±1.31)％;동물체내하류실험교대조조차별명현,대조조종류중량여전이비솔명현고우RPM조[(3.41±0.28)g vs (1.19+0.23)g,100％ (7/7) vs 14.29％ (1/7),P＜0.05],종류증장화전이피현저억제.결론 RPM명현억제인전렬선암세포PC-3적생장급천이,이RPM위기출적전렬선암치료방안가능재림상중구유량호적응용전경.
Objective To observe the effect of rapamycin on the proliferation and cell cycle of prostate cancer cell lines PC3,and to investigate the mechanism that it inhibits the growth and metastasis of prostate cancer cells.Methods PC3 cells were cultured in vitro,and treated with different concentrations (10,25ng/ml) of cycle raparnycine.MTT was used to measure the change of proliferation of PC3 cells.Flow cytometry was used to measure the changes of cell cycle and apoptosis of PC3 cells.Nude mice were used to detect the effect of rapamycin on metastasis.Results The proliferation of PC3 cells was significantly inhibited by rapamycin,and a time- and concentration-dependent relationship was shown,the inhibited rate was(42.23 ± 0.78 ) ％ after 36 h in the group of 25 ng/ml ( P ＜ 0.05 ).Flow cytometry analysis showed rapamycin significantly inhibited the cell cycle,prompted the apoptosis,and increased the number of cells in G0/G1 phase at 36 h with a rate of cell staying at Go/G1 [ (92.17 ± 0.69 ) ％ ] ( P ＜ 0.05 ).The weight of tumors in nude mice in the control group was significantly greater than that in RPM group[ (3.41± 0.28)g vs ( 1.19 ± 0.23 )g] ( P＜ 0.05 ),and metastatic sites of the lung and liver in the control group were significantly mote than the RPM group [ 100％ (7/7) vs 14.29％ ( 1/7 ) ] ( P ＜ 0.05 ).Conclusions Rapamycin significantly inhibits the proliferation and metastasis of osteosarcoma.The rapamycin-based regimen is valuable for clinical application.
